{"title":"Design of Reduction-Responsive Copolymer-Based Nanoparticles for Enhanced Anticancer Drug Delivery","authors":"Haotian Hu, Hongjing Liu, Qingqing Li, Yongpeng Shen, Jialin Lu, Xiaowen Liu, Yu Tong, Yiheng Feng, Qingbo Xu, Yuxiang Tang, Jing Chu, Hang Hu, Tianyu Zhu, Defeng Xu","doi":"10.1007/s10924-024-03403-5","DOIUrl":null,"url":null,"abstract":"<p>Polymer-based nanoparticles with tumor-targeting ability, controlled-release properties and good biocompatibility are of great interest for anticancer drug delivery. Herein, two amphiphilic reduction-responsive copolymers self-assembled nanoparticles (mPEG-Cys-PCL and mPEG-Ami-PCL) along with their inert counterpart (mPEG-Hex-PCL) were prepared and evaluated. These three copolymers were synthesized by conjugating mPEG and PCL with different linkers and characterized by proton nuclear magnetic resonance spectrometry, flourier transform infrared spectrometry and gel permeation chromatography. Nile red (NR) was loaded into the prepared nanoparticles as a model drug to study the in vitro drug release, cellular uptake amount and biodistribution. Dimethylcurcumin (DMC) was loaded into the prepared nanoparticles to study the in vitro antitumor effect. The results show that NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles exhibit glutathione (GSH)-triggered drug release and NR@mPEG-Ami-PCL nanoparticles display enhanced GSH-responsiveness as compared to NR@mPEG-Cys-PCL. Moreover, NR@mPEG-Ami-PCL nanoparticles possess enhanced cellular uptake amount as compared to NR@mPEG-Hex-PCL and NR@mPEG-Cys-PCL nanoparticles. DMC@mPEG-Ami-PCL nanoparticles possess the highest in vitro antitumor effect. In biodistribution study, both NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles show reduced organ distribution and similar tumor accumulation as compared to NR@mPEG-Hex-PCL nanoparticles. The mPEG-Ami-PCL nanoparticles developed in this work show great potential for anticancer drug delivery.</p>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"189 1","pages":""},"PeriodicalIF":4.7000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10924-024-03403-5","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Polymer-based nanoparticles with tumor-targeting ability, controlled-release properties and good biocompatibility are of great interest for anticancer drug delivery. Herein, two amphiphilic reduction-responsive copolymers self-assembled nanoparticles (mPEG-Cys-PCL and mPEG-Ami-PCL) along with their inert counterpart (mPEG-Hex-PCL) were prepared and evaluated. These three copolymers were synthesized by conjugating mPEG and PCL with different linkers and characterized by proton nuclear magnetic resonance spectrometry, flourier transform infrared spectrometry and gel permeation chromatography. Nile red (NR) was loaded into the prepared nanoparticles as a model drug to study the in vitro drug release, cellular uptake amount and biodistribution. Dimethylcurcumin (DMC) was loaded into the prepared nanoparticles to study the in vitro antitumor effect. The results show that NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles exhibit glutathione (GSH)-triggered drug release and NR@mPEG-Ami-PCL nanoparticles display enhanced GSH-responsiveness as compared to NR@mPEG-Cys-PCL. Moreover, NR@mPEG-Ami-PCL nanoparticles possess enhanced cellular uptake amount as compared to NR@mPEG-Hex-PCL and NR@mPEG-Cys-PCL nanoparticles. DMC@mPEG-Ami-PCL nanoparticles possess the highest in vitro antitumor effect. In biodistribution study, both NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles show reduced organ distribution and similar tumor accumulation as compared to NR@mPEG-Hex-PCL nanoparticles. The mPEG-Ami-PCL nanoparticles developed in this work show great potential for anticancer drug delivery.
期刊介绍:
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.