Yingdong Fan, Wuhua Zhang, Jinzhu Zhang, Tao Yang, Naiyu Zhang, Shuang Liang, Jie Dong, Daidi Che
{"title":"RhLHY-RhTPPF-Tre6P Inhibits Flowering in Rosa hybrida Under Insufficient Light by Regulating Sugar Distribution","authors":"Yingdong Fan, Wuhua Zhang, Jinzhu Zhang, Tao Yang, Naiyu Zhang, Shuang Liang, Jie Dong, Daidi Che","doi":"10.1111/pce.15157","DOIUrl":null,"url":null,"abstract":"Light is crucial for flower bud development in plants, serving as both signal and energy source. However, the mechanisms by which daylength and light intensity regulate flowering in modern roses remain unclear. In <i>Rosa hybrida</i> ‘Carola’, insufficient light delays flowering and reduces the sugar content in terminal buds. RNA sequencing identified the <i>Trehalose-6-phosphate phosphatase F</i> (<i>RhTPPF</i>) gene as a key responder to insufficient light, modulating Tre6P metabolism. Overexpression of <i>RhTPPF</i> in rose calli enhanced sugar accumulation and suppressed the synthesis of <i>RhCO/FT</i>. In tobacco, overexpression of <i>RhTPPF</i> delayed the transition from vegetative growth to flowering, while silencing <i>RhTPPF</i> in roses accelerated flowering. Silencing <i>RhTPPF</i> in roses elevated trehalose-6-phosphate (Tre6P) levels and decreased trehalose. Transcriptome data showed that the expression level of <i>RhTPPF</i> was highly correlated with the circadian rhythm gene <i>LATE ELONGATED HYPOCOTYL</i> (<i>RhLHY</i>). Yeast one-hybrid assays, dual luciferase assays and EMSA revealed that <i>RhLHY</i> directly binds to the <i>RhTPPF</i> promoters. Overexpression of <i>RhLHY</i> suppressed flowering, while silencing <i>RhLHY</i> promoted flowering. Furthermore, altering the expression of <i>RhLHY</i> influenced Tre6P synthesis and the expression of sucrose-related transport genes. These findings suggest a RhLHY-RhTPPF-Tre6P regulatory module that maintains sugar balance and inhibits flower formation under reduced light conditions by modulating sugar distribution.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.15157","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Light is crucial for flower bud development in plants, serving as both signal and energy source. However, the mechanisms by which daylength and light intensity regulate flowering in modern roses remain unclear. In Rosa hybrida ‘Carola’, insufficient light delays flowering and reduces the sugar content in terminal buds. RNA sequencing identified the Trehalose-6-phosphate phosphatase F (RhTPPF) gene as a key responder to insufficient light, modulating Tre6P metabolism. Overexpression of RhTPPF in rose calli enhanced sugar accumulation and suppressed the synthesis of RhCO/FT. In tobacco, overexpression of RhTPPF delayed the transition from vegetative growth to flowering, while silencing RhTPPF in roses accelerated flowering. Silencing RhTPPF in roses elevated trehalose-6-phosphate (Tre6P) levels and decreased trehalose. Transcriptome data showed that the expression level of RhTPPF was highly correlated with the circadian rhythm gene LATE ELONGATED HYPOCOTYL (RhLHY). Yeast one-hybrid assays, dual luciferase assays and EMSA revealed that RhLHY directly binds to the RhTPPF promoters. Overexpression of RhLHY suppressed flowering, while silencing RhLHY promoted flowering. Furthermore, altering the expression of RhLHY influenced Tre6P synthesis and the expression of sucrose-related transport genes. These findings suggest a RhLHY-RhTPPF-Tre6P regulatory module that maintains sugar balance and inhibits flower formation under reduced light conditions by modulating sugar distribution.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.