Clinical significance and biological role of claudin-1 in oral squamous cell carcinoma cells

IF 1.9 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-01-01 DOI:10.1016/j.oooo.2024.08.020

Tadayoshi Nobumoto DDS, PhD , Sachiko Yamasaki DDS, PhD , Atsuko Hamada DDS, PhD , Mirai Higaki DDS, PhD , Nanako Ito DDS, PhD , Fumitaka Obayashi DDS, PhD , Yasutaka Ishida DDS, PhD , Tomoaki Hamana DDS, PhD , Tomoaki Shintani DDS, PhD , Ryouji Tani DDS, PhD , Koichi Koizumi DDS, PhD , Souichi Yanamoto DDS, PhD , Yasutaka Hayashido DDS, PhD

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Abstract

Objectives

Claudin (CLD), a major component of tight junctions, is a four-transmembrane protein, and 24 subtypes have been reported in humans. CLD expression is highly tissue-specific; CLD1 has been reported to be expressed in the skin and mucosa. There have been few reports on CLD1 expression and its function in oral cancer.

Materials and methods

This retrospective study immunohistochemically evaluated CLD1 expression as prognostic predictors in 84 participants with oral squamous cell carcinoma (OSCC). Participants were classified as positive or negative based on staining intensity; the clinicopathologic characteristics and survival rates of the two groups were compared. To clarify the biological role of CLD1 in OSCC cells, we examined the effects of CLD1 overexpression on the invasion and proliferation of the OSCC cell line, SCCKN.

Results

We observed the immunohistochemical CLD1 expression in the cell membranes of OSCC cells. The disease-free survival rate was significantly lower in patients with CLD1-positive OSCC than in patients with CLD1-negative OSCC (P < .05). In vitro studies showed that cell proliferative capacity, motility, proteolytic activity, and invasive growth were promoted in CLD1-overexpressing SCCKN cells compared to those in control SCCKN cells.

Conclusion

CLD1 may be a useful and potential prognostic factor for OSCC treatment.

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克劳丁-1 在口腔鳞状细胞癌细胞中的临床意义和生物学作用

Claudin（CLD）是紧密连接的主要成分，是一种四跨膜蛋白，据报道在人类中有 24 种亚型。CLD 的表达具有高度的组织特异性；据报道，CLD1 在皮肤和粘膜中均有表达。有关 CLD1 在口腔癌中的表达及其功能的报道很少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.