Utilization of duckweed-derived biomass for polyhydroxybutyrate production in Escherichia coli via dual enzymatic saccharification using amylase and cellulase complex.

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-12 DOI:10.1093/bbb/zbae127
Toshihiko Ooi,Rikako Sato,Ken'ichiro Matsumoto
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Abstract

Duckweed is a rapid-growing plant with a high starch and low lignin content. The duckweed was saccharified via dual enzymatic treatment using amylase and cellulase complex. The duckweed-derived glucose was utilized for polyhydroxybutyrate (PHB) production in engineered Escherichia coli. In addition, the low concentration supplementation of the duckweed extract promoted cell growth compared to the analytical grade glucose.
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通过使用淀粉酶和纤维素酶复合物进行双酶糖化,在大肠杆菌中利用浮萍生物质生产多羟基丁酸。
浮萍是一种生长迅速的植物,淀粉含量高,木质素含量低。利用淀粉酶和纤维素酶复合物对浮萍进行双重酶处理,使其糖化。利用浮萍提取的葡萄糖在工程大肠杆菌中生产聚羟基丁酸(PHB)。此外,与分析级葡萄糖相比,低浓度补充浮萍提取物可促进细胞生长。
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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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