Spatiotemporal Mapping of the Oxytocin Receptor at Single-Cell Resolution in the Postnatally Developing Mouse Brain

IF 5.9 2区 医学 Q1 NEUROSCIENCES Neuroscience bulletin Pub Date : 2024-09-15 DOI:10.1007/s12264-024-01296-x
Hao Li, Ying Li, Ting Wang, Shen Li, Heli Liu, Shuyi Ning, Wei Shen, Zhe Zhao, Haitao Wu
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Abstract

The oxytocin receptor (OXTR) has garnered increasing attention for its role in regulating both mature behaviors and brain development. It has been established that OXTR mediates a range of effects that are region-specific or period-specific. However, the current studies of OXTR expression patterns in mice only provide limited help due to limitations in resolution. Therefore, our objective was to generate a comprehensive, high-resolution spatiotemporal expression map of Oxtr mRNA across the entire developing mouse brain. We applied RNAscope in situ hybridization to investigate the spatiotemporal expression pattern of Oxtr in the brains of male mice at six distinct postnatal developmental stages (P7, P14, P21, P28, P42, P56). We provide detailed descriptions of Oxtr expression patterns in key brain regions, including the cortex, basal forebrain, hippocampus, and amygdaloid complex, with a focus on the precise localization of Oxtr+ cells and the variance of expression between different neurons. Furthermore, we identified some neuronal populations with high Oxtr expression levels that have been little studied, including glutamatergic neurons in the ventral dentate gyrus, Vgat+Oxtr+ cells in the basal forebrain, and GABAergic neurons in layers 4/5 of the cortex. Our study provides a novel perspective for understanding the distribution of Oxtr and encourages further investigations into its functions.

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以单细胞分辨率绘制产后发育小鼠大脑中催产素受体的时空分布图
催产素受体(OXTR)在调节成熟行为和大脑发育方面的作用日益受到关注。目前已经确定,催产素受体介导了一系列具有区域特异性或时期特异性的效应。然而,由于分辨率的限制,目前对小鼠 OXTR 表达模式的研究只能提供有限的帮助。因此,我们的目标是绘制整个发育中小鼠大脑中 OXTR mRNA 的全面、高分辨率时空表达图谱。我们应用 RNAscope 原位杂交技术研究了雄性小鼠出生后六个不同发育阶段(P7、P14、P21、P28、P42 和 P56)大脑中 Oxtr 的时空表达模式。我们详细描述了Oxtr在大脑皮层、基底前脑、海马和杏仁复合体等主要脑区的表达模式,重点关注Oxtr+细胞的精确定位以及不同神经元之间的表达差异。此外,我们还发现了一些Oxtr高表达水平的神经元群,这些神经元群的研究很少,包括腹侧齿状回的谷氨酸能神经元、基底前脑的Vgat+Oxtr+细胞和大脑皮层4/5层的GABA能神经元。我们的研究为了解 Oxtr 的分布提供了一个新的视角,有助于进一步研究其功能。
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来源期刊
Neuroscience bulletin
Neuroscience bulletin NEUROSCIENCES-
CiteScore
7.20
自引率
16.10%
发文量
163
审稿时长
6-12 weeks
期刊介绍: Neuroscience Bulletin (NB), the official journal of the Chinese Neuroscience Society, is published monthly by Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) and Springer. NB aims to publish research advances in the field of neuroscience and promote exchange of scientific ideas within the community. The journal publishes original papers on various topics in neuroscience and focuses on potential disease implications on the nervous system. NB welcomes research contributions on molecular, cellular, or developmental neuroscience using multidisciplinary approaches and functional strategies. We feature full-length original articles, reviews, methods, letters to the editor, insights, and research highlights. As the official journal of the Chinese Neuroscience Society, which currently has more than 12,000 members in China, NB is devoted to facilitating communications between Chinese neuroscientists and their international colleagues. The journal is recognized as the most influential publication in neuroscience research in China.
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