Gibberellin 2-oxidase 1(CsGA2ox1) involved gibberellin biosynthesis regulates sprouting time in camellia sinensis

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL ACS Applied Energy Materials Pub Date : 2024-09-17 DOI:10.1186/s12870-024-05589-1

Ziyuan Qiu, Wenhui Guo, Qian Yu, Dongxue Li, Mengjie Zhao, Han Lv, Xuewen Hua, Yu Wang, Qingping Ma, Zhaotang Ding

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Abstract

Tea is an important cash crop and buds are its main product. To elucidate the molecular mechanism of the sprouting time of tea plants, ‘Yuchunzao’, which was an early sprouting tea cultivar, was studied. ‘Echa 1’, sprout one week later than ‘Yuchunzao’ in spring, was used as the control. A total of 26 hormonal compounds and its derivatives in tea plants were qualified by using Ultra Performance Liquid Chromatography-Tandem mass spectrometry (UPLC-MS/MS). The result showed that GA20, GA3 and ICA were significantly different in ‘Yuchunzao’ than in ‘Echa 1’, with GA20 and GA3 up-regulated and ICA down-regulated. Based on the Illumina platform, transcriptome analysis revealed a total of 5,395 differentially expressed genes (DEGs). A diterpenoid biosynthesis related gene, gibberellin 2-oxidase 1 (CsGA2ox1), was downregulated in ‘Yuchunzao’ compared to ‘Echa 1’. CsGA2ox1 regulate the transformation of GA different forms in plants. The relative expression of CsGA2ox1 showed an adverse trend with the content of GA20 and GA3. Our results suggest that down regulation of CsGA2ox1 resulted in the accumulation of GA3 and GA20, and then promoted sprout of ‘Yuchunzao’. This study provides theoretical basis of tea plants sprout and guides the tea breeding in practice.

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赤霉素 2-氧化酶 1（CsGA2ox1）参与赤霉素的生物合成，调控山茶花的萌芽时间

茶叶是一种重要的经济作物，芽是其主要产品。为了阐明茶树发芽时间的分子机制，研究人员对早发芽茶树品种'玉春早'进行了研究。以春季比 "玉春早 "晚发芽一周的 "二茶 1 号 "为对照。采用超高效液相色谱-串联质谱法（UPLC-MS/MS）对茶树中的 26 种激素化合物及其衍生物进行了鉴定。结果表明，与 "二茶1号 "相比，"玉春早 "中的GA20、GA3和ICA有显著差异，其中GA20和GA3上调，ICA下调。基于 Illumina 平台的转录组分析共发现了 5,395 个差异表达基因（DEGs）。与 "二茶 1 号 "相比，"玉春早 "中与二萜生物合成相关的基因赤霉素 2-oxidase 1（CsGA2ox1）被下调。CsGA2ox1 调控植物体内不同形式 GA 的转化。CsGA2ox1 的相对表达量与 GA20 和 GA3 的含量呈负相关趋势。我们的研究结果表明，CsGA2ox1的下调导致了GA3和GA20的积累，进而促进了'玉春早'的萌发。本研究为茶树萌芽提供了理论依据，并指导了茶树育种实践。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.