Plant growth-promoting microorganisms drive K strategists through deterministic processes to alleviate biological stress caused by Fusarium oxysporum

IF 6.1 1区 生物学 Q1 MICROBIOLOGY Microbiological research Pub Date : 2024-09-18 DOI:10.1016/j.micres.2024.127911
Liangyang Mao , Bo Yin , Zeming Ye , Jie Kang , Rui Sun , Zhenchao Wu , Jingping Ge , Wenxiang Ping
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Abstract

Soybean root rot, caused by soil-borne pathogens such as Fusarium oxysporum, frequently occurs in Northeast China and leads to a decline in soil health and becoming a bottleneck for soybean yield in the region. To address this issue, applying beneficial microorganisms and altering soil microbial community structure have become effective strategies. In this study, the 90-day soybean pot experiment was conducted to explore the assembly process and life strategy selection of bacterial communities in the rhizosphere of healthy (inoculated with Funneliformis mosseae, F group and treated with Pseudomonas putida, P group) and diseased (inoculated with F. oxysporum, O group) soybean plants, as well as the recovery effect of beneficial microorganisms on soil-borne diseases (combined treatments OP and OF). Results indicated that in healthy soils (P and F), microbial community assembly process in the soybean rhizosphere was entirely governed by heterogeneous selection (HeS, 100 %). However, inoculated with P. putida (OP) was primarily driven by stochastic processes (HeS 40 %, dispersal limitation (DL) 60 %), and the F. mosseae treatment (OF) predominantly followed a deterministic process (HeS 89 %, DL 11 %) in diseased soils. Inoculation of plant growth-promoting microorganisms (PGPMs) in diseased soil drove the life strategy of the rhizosphere bacterial community from r- to K-strategy, evident from the lower rRNA operon (rrn) copy numbers (O 3.7, OP 2.1, OF 2.3), higher G+ to G- ratios (O 0.47, OP 0.58, OF 0.57), and a higher abundance of oligotrophs (O 50 %, OP 53 %, OF 54 %). In healthy (P and F) and diseased (O, OP, OF) rhizosphere soils, OTU820, OTU6142, and OTU8841 under the K-strategy, and OTU6032 and OTU6917 under the r-strategy, which served as keystone species, had a significant promoting relationship with plant biomass and defense capabilities ( p <0.05). Additionally, inoculation of PGPMs improved autotoxin degradation and positively correlated with bacterial life strategies in both healthy and diseased soils (P, F, OP and OF) ( p <0.05). These findings enhance our understanding of soil-microbe interactions and offer new insights and precise control measures for soybean disease management and soil environment remediation.

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植物生长促进微生物通过决定性过程驱动钾策略因子,以减轻镰刀菌造成的生物胁迫
大豆根腐病是由镰刀菌等土传病原菌引起的,在中国东北地区经常发生,导致土壤健康状况下降,成为该地区大豆产量的瓶颈。为解决这一问题,施用有益微生物和改变土壤微生物群落结构已成为有效的策略。本研究通过为期 90 天的大豆盆栽实验,探讨了健康大豆植株(接种 Funneliformis mosseae,F 组,用 Pseudomonas putida 处理,P 组)和患病大豆植株(接种 F. oxysporum,O 组)根瘤菌群落的组装过程和生命策略选择,以及有益微生物对土传病害(OP 和 OF 组合处理)的恢复效果。结果表明,在健康土壤(P 和 F)中,大豆根圈微生物群落的形成过程完全受异质性选择(HeS,100%)的控制。然而,在病害土壤中,接种 P. putida(OP)主要由随机过程驱动(HeS 40 %,扩散限制 (DL) 60 %),而 F. mosseae 处理(OF)则主要遵循确定性过程(HeS 89 %,DL 11 %)。在病害土壤中接种促进植物生长的微生物(PGPMs)后,根瘤菌群落的生活战略从 r 战略转变为 K 战略,这从较低的 rRNA 操作子(rrn)拷贝数(O 3.7、OP 2.1、OF 2.3)、较高的 G+ 与 G- 比率(O 0.47、OP 0.58、OF 0.57)和较高的寡养菌丰度(O 50%、OP 53%、OF 54%)中可以看出。在健康(P 和 F)和病害(O、OP、OF)根瘤土壤中,K 策略下的 OTU820、OTU6142 和 OTU8841,以及 r 策略下的 OTU6032 和 OTU6917 作为关键种,与植物的生物量和防御能力有显著的促进关系 ( p <0.05)。此外,接种 PGPMs 可改善自毒素降解,并与健康土壤和病害土壤中的细菌生活策略(P、F、OP 和 OF)呈正相关(p <0.05)。这些发现加深了我们对土壤-微生物相互作用的理解,为大豆病害管理和土壤环境修复提供了新的见解和精确的控制措施。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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