Prevalence status and detection of benzimidazole resistance using AS-PCR in Haemonchus contortus of goats from Marathwada region, Maharashtra, India

IF 1.4 Q3 PARASITOLOGY Veterinary parasitology, regional studies and reports Pub Date : 2024-09-19 DOI:10.1016/j.vprsr.2024.101119

Vishal Bhagat , Chandrakant Bhong , Bapurao Khillare , Nitin Jadhav , Mahima Narawade , Gajendra Khandekar , Shahir Gaikwad , Baliram Katkade , Anil Kumar Sharma , Gajanan Chigure

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Abstract

This study examined Haemonchus contortus prevalence and benzimidazole resistance in eight districts of Marathwada, Maharashtra, India. A comprehensive investigation of 264 abomasa of goats collected from abattoirs and goats necropsied at the College of Veterinary Sciences and Animal Husbandry, Parbhani, revealed 21.21 % a prevalence of H. contortus. The incidence of H. contortus did not vary much across seasons and it was highest in summer (23.42 %), followed by monsoon (22.89 %), and lowest in winter (15.71 %). Statistically non-significant (p < 0.05) prevalence was observed in male and female animals. A detailed examination of 168 adult H. contortus worms from eight districts revealed the presence of all conceivable genotypes including homozygous resistant (rr), susceptible (SS), and heterozygous (Sr) BZ susceptible genotypes. The rr was the most frequent at 50 %, followed by SS at 27 % and Sr at 22 %. The presence of the SNP was observed in in all eight randomly selected and sequenced samples.

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使用 AS-PCR 技术检测印度马哈拉施特拉邦马拉瓦达地区山羊口蹄疫的流行状况和苯并咪唑抗药性

本研究调查了印度马哈拉施特拉邦马拉瓦达八个地区的口蹄疫流行情况和苯并咪唑抗药性。对从屠宰场收集的 264 只山羊腹腔和在帕尔巴尼兽医科学和畜牧学院进行的山羊尸体解剖进行了全面调查，结果显示，山羊腹腔线虫病的流行率为 21.21%。感染率在不同季节变化不大，夏季最高（23.42%），其次是季风季节（22.89%），冬季最低（15.71%）。在雄性和雌性动物中观察到的流行率无统计学意义（p < 0.05）。对 8 个地区的 168 只成虫进行的详细检查显示，存在所有可能的基因型，包括同源抗性基因型（rr）、易感基因型（SS）和异源 BZ 易感基因型（Sr）。Rr 最常见，占 50%，其次是 SS，占 27%，Sr 占 22%。在随机选择并测序的所有八个样本中都观察到了 SNP 的存在。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).