Periplaneta americana extract CII-3 triggers cell senescence through activating ROS-p38 MAPK-p53 signaling pathway in SKOV3 cells

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2024-12-01 Epub Date: 2024-09-13 DOI:10.1016/j.tice.2024.102561

Lu Tian , Heng Liu , Yue Zhou , Chenggui Zhang , Ziying Bi , Ting Wu

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Abstract

This study aimed to investigate effect of Periplaneta americana extract CII-3 (CII-3) in senescence of SKOV3 cells. Proliferation, colony forming and cell senescence of SKOV3 cells were determined. ROS production was evaluated by flow cytometry. Transcription of telomerase (TERT), p38 MAPK and p53 gene and protein expression of p-p38 MAPK and p-p53, were identified. CII-3 at different concentrations significantly inhibited SKOV3 proliferation, and 80 μg/ml demonstrated the highest inhibitory effect. CII-3 significantly blocked cell cycle in G0/G1 phase (P<0.01) and reduced colony forming efficiency (P<0.001) of SKOV3 cells compared to those in Control group. CII-3 significantly increased SA-β-Gal positive staining SKOV3 cells (P<0.001) and reduced mitochondrial membrane potential (P<0.01) compared to those in Control group. CII-3 markedly decreased TERT gene transcription of SKOV3 cells compared to that in Control group (P<0.001). CII-3 also triggered significantly higher ROS levels in SKOV3 cells compared to that in Control group (P<0.001). CII-3 significantly increased p-p38 MAPK (P<0.001), p-p53 (P<0.001) and p21 (P<0.001) expressions of SKOV3 cells compared to those in Control group. In conclusion, CII-3 triggered cell senescence of SKOV3 cells through activating ROS-p38 MAPK-p53 signaling pathway. This study would provide a promising strategy for inhibiting cancer cell proliferation by including cell senescence.

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Periplaneta americana 提取物 CII-3 通过激活 SKOV3 细胞中的 ROS-p38 MAPK-p53 信号通路引发细胞衰老

本研究旨在探讨美洲长春花提取物CII-3（CII-3）对SKOV3细胞衰老的影响。研究测定了 SKOV3 细胞的增殖、集落形成和细胞衰老。流式细胞术评估了 ROS 的产生。鉴定了端粒酶（TERT）、p38 MAPK 和 p53 基因的转录以及 p-p38 MAPK 和 p-p53 的蛋白表达。不同浓度的 CII-3 均能明显抑制 SKOV3 的增殖，其中 80 μg/ml 的抑制作用最强。与对照组相比，CII-3能明显阻滞SKOV3细胞G0/G1期的细胞周期（P<0.01），降低集落形成效率（P<0.001）。与对照组相比，CII-3 能明显增加 SKOV3 细胞 SA-β-Gal 阳性染色（P<0.001）并降低线粒体膜电位（P<0.01）。与对照组相比，CII-3 显著降低了 SKOV3 细胞的 TERT 基因转录（P<0.001）。与对照组相比，CII-3 在 SKOV3 细胞中引发的 ROS 水平也明显升高（P<0.001）。与对照组相比，CII-3 能明显增加 SKOV3 细胞中 p-p38 MAPK（P<0.001）、p-p53（P<0.001）和 p21（P<0.001）的表达。总之，CII-3通过激活ROS-p38 MAPK-p53信号通路引发SKOV3细胞衰老。这项研究将为通过细胞衰老抑制癌细胞增殖提供一种有前景的策略。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.