Plasma-derived extracellular vesicles (EVs) as biomarkers of sepsis in burn patients via label-free Raman spectroscopy

IF 15.5 1区 医学 Q1 CELL BIOLOGY Journal of Extracellular Vesicles Pub Date : 2024-09-19 DOI:10.1002/jev2.12506
Hannah J. O'Toole, Neona M. Lowe, Vishalakshi Arun, Anna V. Kolesov, Tina L. Palmieri, Nam K. Tran, Randy P. Carney
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Abstract

Sepsis following burn trauma is a global complication with high mortality, with ∼60% of burn patient deaths resulting from infectious complications. Diagnosing sepsis is complicated by confounding clinical manifestations of the burn injury, and current biomarkers lack the sensitivity and specificity required for prompt treatment. There is a strong rationale to assess circulating extracellular vesicles (EVs) from patient liquid biopsy as sepsis biomarkers due to their release by pathogens from bacterial biofilms and roles in the subsequent immune response. This study applies Raman spectroscopy to patient plasma-derived EVs for rapid, sensitive, and specific detection of sepsis in burn patients, achieving 97.5% sensitivity and 90.0% specificity. Furthermore, spectral differences between septic and non-septic burn patient EVs could be traced to specific glycoconjugates of bacterial strains associated with sepsis morbidity. This work illustrates the potential application of EVs as biomarkers in clinical burn trauma care and establishes Raman analysis as a fast, label-free method to specifically identify features of bacterial EVs relevant to infection amongst the host background.

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通过无标记拉曼光谱将血浆源性细胞外囊泡 (EV) 作为烧伤患者败血症的生物标记物
烧伤创伤后败血症是一种全球性并发症,死亡率很高,60%的烧伤患者死于感染并发症。脓毒症的诊断因烧伤的临床表现而变得复杂,目前的生物标志物缺乏及时治疗所需的灵敏度和特异性。由于病原体从细菌生物膜中释放出细胞外囊泡 (EV),并在随后的免疫反应中发挥作用,因此将患者液体生物样本中的循环细胞外囊泡 (EV) 作为败血症生物标志物进行评估具有很强的合理性。本研究将拉曼光谱应用于患者血浆中的 EVs,以快速、灵敏、特异地检测烧伤患者的败血症,灵敏度达 97.5%,特异度达 90.0%。此外,脓毒症和非脓毒症烧伤患者 EVs 之间的光谱差异可追溯到与脓毒症发病率相关的细菌菌株的特异性糖结合物。这项工作说明了在临床烧伤创面护理中将 EVs 作为生物标记物的潜在应用价值,并证明拉曼分析是一种快速、无标记的方法,可在宿主背景中特异性地识别与感染相关的细菌 EVs 特征。
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来源期刊
Journal of Extracellular Vesicles
Journal of Extracellular Vesicles Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
27.30
自引率
4.40%
发文量
115
审稿时长
12 weeks
期刊介绍: The Journal of Extracellular Vesicles is an open access research publication that focuses on extracellular vesicles, including microvesicles, exosomes, ectosomes, and apoptotic bodies. It serves as the official journal of the International Society for Extracellular Vesicles and aims to facilitate the exchange of data, ideas, and information pertaining to the chemistry, biology, and applications of extracellular vesicles. The journal covers various aspects such as the cellular and molecular mechanisms of extracellular vesicles biogenesis, technological advancements in their isolation, quantification, and characterization, the role and function of extracellular vesicles in biology, stem cell-derived extracellular vesicles and their biology, as well as the application of extracellular vesicles for pharmacological, immunological, or genetic therapies. The Journal of Extracellular Vesicles is widely recognized and indexed by numerous services, including Biological Abstracts, BIOSIS Previews, Chemical Abstracts Service (CAS), Current Contents/Life Sciences, Directory of Open Access Journals (DOAJ), Journal Citation Reports/Science Edition, Google Scholar, ProQuest Natural Science Collection, ProQuest SciTech Collection, SciTech Premium Collection, PubMed Central/PubMed, Science Citation Index Expanded, ScienceOpen, and Scopus.
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