Characteristics of the Serum Microbiota and Circulating Metabolites in Cynomolgus Monkeys With Spontaneous Type 2 Diabetes Mellitus

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Journal of Medical Primatology Pub Date : 2024-09-22 DOI:10.1111/jmp.12738

Chaoyang Tian, Qunhui Yang, Haizhou Lv, Feng Yue

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Abstract

Background

Type 2 diabetes mellitus (T2DM) is widely recognized as a serious global public health concern with a substantial economic burden on patients, their families, and society. Accumulating evidence suggests that an etiologic role for serum microbiota and circulating metabolites in the pathogenesis of T2DM. This study aims to characterize the serum microbiota and circulating metabolites in cynomolgus monkeys with spontaneous T2DM, and provide a reference for the diagnosis and treatment of clinical T2DM.

Methods

We collected serum samples of the 14 cynomolgus monkeys (15–20 years old, male) for serum microbiota analysis by 16S rRNA gene V3-V4 region amplicon sequencing and circulating metabolites analysis by ultra-high-performance liquid chromatography–tandem mass spectrometry, of which seven were spontaneous T2DM cynomolgus monkeys and seven were healthy controls.

Results

Our results showed that the serum microbiota abundance and diversity were significantly increased in cynomolgus monkeys with spontaneous T2DM compared to healthy controls, the phyla of Cyanobacteria and Chloroflexi and the genera of Lactobacillus, rhodobacter and collinsella were also significantly increased. A total of 114 serum differentially expressed metabolites (DEMs) were identified, of which 22 were selected as potential biomarkers candidates related to spontaneous T2DM in cynomolgus monkeys by multivariate data analysis. In addition, serum levels of total SCFAs, acetate, butyrate, caproate, isobutyrate, and isovalerate were also significantly increased in the present study. The correlation network analyses have selected certain key DEMs, such as D-Psicose, 4-Oxoproline, D-Glutamine, and Hydroxyphenyllactic acid, which may serve as potential biomarkers for distinguishing between T2DM and healthy controls.

Conclusion

Our results provide preliminary insights on perturbed serum microbiota and circulating metabolites of cynomolgus monkeys with spontaneous T2DM. These findings would be useful to develop microbiota-based strategies for T2DM prevention and control.

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自发性 2 型糖尿病猴血清微生物群和循环代谢物的特征

背景 2 型糖尿病（T2DM）被广泛认为是一个严重的全球公共卫生问题，给患者、其家庭和社会造成了巨大的经济负担。越来越多的证据表明，血清微生物群和循环代谢物在 T2DM 的发病机制中起着致病作用。本研究旨在分析自发性 T2DM 猴的血清微生物群和循环代谢物的特征，为临床 T2DM 的诊断和治疗提供参考。方法采集14只猴（15-20岁，雄性）的血清样本，采用16S rRNA基因V3-V4区扩增子测序法进行血清微生物群分析，并采用超高效液相色谱-串联质谱法进行循环代谢物分析，其中7只为自发性T2DM猴，7只为健康对照组。结果我们的研究结果表明，与健康对照组相比，自发性 T2DM 猴的血清微生物群丰度和多样性显著增加，蓝藻门、绿藻门以及乳酸杆菌属、红杆菌属和柯林斯菌属也显著增加。通过多变量数据分析，共鉴定出114种血清差异表达代谢物（DEMs），其中22种被选为与猴自发性T2DM相关的潜在候选生物标志物。此外，在本研究中，血清中总 SCFAs、乙酸盐、丁酸盐、己酸盐、异丁酸盐和异戊酸盐的水平也显著升高。相关网络分析筛选出了一些关键的 DEMs，如 D-Psicose、4-Oxoproline、D-谷氨酰胺和羟苯基乳酸，它们可作为区分 T2DM 和健康对照组的潜在生物标志物。结论我们的研究结果提供了关于自发性 T2DM 猴血清微生物群和循环代谢物紊乱的初步见解。这些发现将有助于制定基于微生物群的 T2DM 预防和控制策略。

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.