Recombinant outer membrane protein Lipl41 from Leptospira interrogans robust immune responses in mice model.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI:10.18502/ijm.v16i4.16314

Narges Golab, Pejvak Khaki, Majid Tebianian, Majid Esmaelizad, Naser Harzandi

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Abstract

Background and objectives: Leptospirosis is an infectious zoonotic disease that can result in severe complications. It is widespread, especially in hot and humid climates such as the northern region of Iran. The immune responses to leptospirosis are multifaceted. Lipl41 is an outer membrane protein that is expressed during infection and is highly conserved among pathogenic species. This makes it a good candidate for diagnosis and induction of specific immune responses. The aim of the present study was to evaluate immune responses against recombinant Lipl41 in mice.

Materials and methods: After immunizing of different groups of mice with recombinant Lipl41 (rLipl41), the levels of specific antibodies and cytokine profiles interferon-gamma/interleukin-4 (IFN-γ/IL-4) were measured.

Results: The results revealed that rLipl41 showed a significant increase in antibody levels compared with the control groups (P< 0.05). Although the level of IL-4 in the groups that received Lipl41 was similar to that in the other control groups, the IFN-γ levels showed a significant increase (P<0.05).

Conclusion: It has been concluded that recombinant Lipl41 protein could strongly stimulate specific immune responses and be considered a potential candidate for vaccine development and diagnostic research.

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审讯钩端螺旋体的重组外膜蛋白 Lipl41 在小鼠模型中产生了强有力的免疫反应。

背景和目的：钩端螺旋体病是一种可导致严重并发症的人畜共患传染病。它广泛流行，尤其是在伊朗北部地区等炎热潮湿的气候条件下。钩端螺旋体病的免疫反应是多方面的。Lipl41 是一种在感染期间表达的外膜蛋白，在致病物种之间高度保守。这使其成为诊断和诱导特异性免疫反应的良好候选蛋白。本研究旨在评估小鼠对重组 Lipl41 的免疫反应：用重组 Lipl41（rLipl41）免疫不同组别的小鼠后，测定特异性抗体和细胞因子γ干扰素/白细胞介素-4（IFN-γ/IL-4）的水平：结果：结果显示，与对照组相比，rLipl41 的抗体水平显著增加（P< 0.05）。虽然接受 Lipl41 治疗组的 IL-4 水平与其他对照组相近，但 IFN-γ 水平却显著增加（PC结论：Lipl41 重组人免疫球蛋白可显著提高抗体水平：结论：重组 Lipl41 蛋白能强烈刺激特异性免疫反应，可作为疫苗开发和诊断研究的潜在候选物质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.