Enhancing antioxidant properties of lime juice powder through polyelectrolyte microparticles of chitosan-alginate: Formulation, characterization and stability study.

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2024-07-01 Epub Date: 2024-07-22 DOI:10.4103/JAPTR.JAPTR_556_23
Nur Rahmiati, Retno Sari, Tutik Sri Wahyuni, Maria Lucia Ardhani Dwi Lestari
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Abstract

Lime (Citrus aurantifolia) juice was reported to contain ascorbic acid (AA) and flavonoids, which has bioactivity as antioxidants. To develop an antioxidant product, improving its stability is necessary due to the perishable characteristics of compounds in lime. Therefore, the formulation of polyelectrolyte microparticles using chitosan and alginate was conducted to overcome the weaknesses. This study aims to evaluate the effect of various chitosan, alginate, and lime juice powder (LJP) concentrations on the physical characteristics and antioxidant activity of LJP encapsulated in chitosan-alginate microparticles (CALM). Microparticles with various concentrations of chitosan and alginate were prepared by ionic gelation method using CaCl2 as a crosslinker. The microparticles were evaluated for its physical properties and its antioxidant activity using 2-2-diphenyl-1-picrylhydrazyl reagent. A one-way ANOVA test and Tukey's honest significant difference post hoc were used to determine the effect of LJP amount on the antioxidant activity. The highest AA content in CALM was 0.14 mg/100 mg, with a % encapsulation efficiency of 18.38% ± 0.02%. Antioxidant activity tests revealed that LJP possessed the strong antioxidant activity with an IC50 value of 32.59 μg/mL, whereas IC50 values of the microparticles ranged from 24.79 ± 0.03 μg/mL to 39.96 ± 0.07 μg/mL. During storage, the IC50 of LJP decreased from 32.59 ± 0.13 μg/mL to 65.53 ± 0.03 μg/mL, whereas the IC50 of microparticles remained stable. This study concluded that the chitosan-alginate polyelectrolyte microparticle formulation can improve and protect LJP's antioxidant activity.

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通过壳聚糖-精氨酸聚电解质微颗粒提高酸橙汁粉的抗氧化性:配方、表征和稳定性研究
据报道,酸橙(Citrus aurantifolia)果汁含有抗坏血酸(AA)和类黄酮,具有抗氧化剂的生物活性。由于酸橙中的化合物具有易腐烂的特点,要开发抗氧化剂产品,就必须提高其稳定性。因此,研究人员利用壳聚糖和海藻酸盐配制了聚电解质微颗粒,以克服上述弱点。本研究旨在评估不同浓度的壳聚糖、海藻酸盐和酸橙汁粉(LJP)对包裹在壳聚糖-海藻酸盐微颗粒(CALM)中的酸橙汁粉的物理特性和抗氧化活性的影响。使用 CaCl2 作为交联剂,通过离子凝胶法制备了不同浓度的壳聚糖和海藻酸盐微颗粒。使用 2-2-二苯基-1-苦基肼试剂对微粒的物理性质和抗氧化活性进行了评估。采用单因素方差分析和 Tukey's 诚实显著性差异检验来确定 LJP 含量对抗氧化活性的影响。CALM 中的 AA 含量最高,为 0.14 mg/100 mg,封装效率为 18.38% ± 0.02%。抗氧化活性测试表明,LJP 具有较强的抗氧化活性,其 IC50 值为 32.59 μg/mL,而微粒的 IC50 值在 24.79 ± 0.03 μg/mL 到 39.96 ± 0.07 μg/mL 之间。在储存过程中,LJP 的 IC50 从 32.59 ± 0.13 μg/mL 降至 65.53 ± 0.03 μg/mL,而微颗粒的 IC50 保持稳定。这项研究得出结论,壳聚糖-精氨酸聚电解质微颗粒配方可以提高和保护LJP的抗氧化活性。
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来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
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