Impact of continuous topical oxygen therapy on biofilm gene expression in a porcine tissue model.

IF 1.5 4区医学 Q3 DERMATOLOGY Journal of wound care Pub Date : 2024-09-02 DOI:10.12968/jowc.2024.0213

Christopher Ball, Hannah Jones, Hannah Thomas, Emma Woodmansey, Windy Cole, Gregory Schultz

{"title":"Impact of continuous topical oxygen therapy on biofilm gene expression in a porcine tissue model.","authors":"Christopher Ball, Hannah Jones, Hannah Thomas, Emma Woodmansey, Windy Cole, Gregory Schultz","doi":"10.12968/jowc.2024.0213","DOIUrl":null,"url":null,"abstract":"Objective: The effect of continuous topical oxygen therapy (cTOT) on Pseudomonas aeruginosa biofilm gene transcription profiles following inoculation onto porcine skin, using a customised molecular assay was determined.Method: Sterilised porcine skin explants were inoculated with Pseudomonas aeruginosa in triplicate: 0 hours as negative control; 24 hours cTOT device on; 24 hours cTOT device off. The oxygen delivery system of the cTOT device was applied to the inoculated tissue and covered with a semi-occlusive dressing. All samples were incubated at 37±2°C for 24 hours, with the 0 hours negative control inoculated porcine skin samples recovered immediately. Planktonic suspensions and porcine skin biopsy samples were taken at 0 hours and 24 hours. Samples were processed and quantifiably assessed using gene specific reverse transcription-quantitative polymerase chain reaction assays for a panel of eight Pseudomonas aeruginosa genes (16S, pelA, pslA, rsaL, pcrV, pscQ, acpP, cbrA) associated with biofilm formation, quorum sensing, protein secretion/translocation and metabolism.Results: Transcriptional upregulation of pelA, pcrV and acpP, responsible for intracellular adhesion, needletip protein production for type-3 secretion systems and fatty acid synthesis during proliferation, respectively, was observed when the cTOT device was switched on compared to when the device was switched off. Data suggest increased metabolic activity within bacterial cells following cTOT treatment.Conclusion: cTOT is an adjunctive therapy that supports faster healing and pain reduction in non-healing hypoxic wounds. Oxygen has previously been shown to increase susceptibility of biofilms to antibiotics through enhancing metabolism. Observed gene expression changes highlighted the impact of cTOT on biofilms, potentially influencing antimicrobial treatment success in wounds. Further in vitro and clinical investigations are warranted.","PeriodicalId":17590,"journal":{"name":"Journal of wound care","volume":"33 9","pages":"702-707"},"PeriodicalIF":1.5000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of wound care","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.12968/jowc.2024.0213","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: The effect of continuous topical oxygen therapy (cTOT) on Pseudomonas aeruginosa biofilm gene transcription profiles following inoculation onto porcine skin, using a customised molecular assay was determined.

Method: Sterilised porcine skin explants were inoculated with Pseudomonas aeruginosa in triplicate: 0 hours as negative control; 24 hours cTOT device on; 24 hours cTOT device off. The oxygen delivery system of the cTOT device was applied to the inoculated tissue and covered with a semi-occlusive dressing. All samples were incubated at 37±2°C for 24 hours, with the 0 hours negative control inoculated porcine skin samples recovered immediately. Planktonic suspensions and porcine skin biopsy samples were taken at 0 hours and 24 hours. Samples were processed and quantifiably assessed using gene specific reverse transcription-quantitative polymerase chain reaction assays for a panel of eight Pseudomonas aeruginosa genes (16S, pelA, pslA, rsaL, pcrV, pscQ, acpP, cbrA) associated with biofilm formation, quorum sensing, protein secretion/translocation and metabolism.

Results: Transcriptional upregulation of pelA, pcrV and acpP, responsible for intracellular adhesion, needletip protein production for type-3 secretion systems and fatty acid synthesis during proliferation, respectively, was observed when the cTOT device was switched on compared to when the device was switched off. Data suggest increased metabolic activity within bacterial cells following cTOT treatment.

Conclusion: cTOT is an adjunctive therapy that supports faster healing and pain reduction in non-healing hypoxic wounds. Oxygen has previously been shown to increase susceptibility of biofilms to antibiotics through enhancing metabolism. Observed gene expression changes highlighted the impact of cTOT on biofilms, potentially influencing antimicrobial treatment success in wounds. Further in vitro and clinical investigations are warranted.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

持续局部氧疗对猪组织模型中生物膜基因表达的影响。

目的：采用定制的分子检测方法，确定持续局部氧疗（cTOT）对接种到猪皮肤上的铜绿假单胞菌生物膜基因转录谱的影响：方法：将消毒后的猪皮肤外植体接种铜绿假单胞菌，一式三份：0 小时为阴性对照；24 小时 cTOT 设备开启；24 小时 cTOT 设备关闭。将 cTOT 装置的氧气输送系统应用于接种组织，并用半封闭敷料覆盖。所有样本在 37±2°C 下培养 24 小时，0 小时阴性对照接种猪皮肤样本立即恢复。在 0 小时和 24 小时时采集浮游悬浮液和猪皮肤活检样本。对样本进行处理，并使用基因特异性反转录-定量聚合酶链反应测定法对与生物膜形成、法定量感应、蛋白质分泌/转运和新陈代谢有关的八个铜绿假单胞菌基因（16S、pelA、pslA、rsaL、ppcrV、pscQ、acpP、ccrA）进行定量评估：结果：与关闭 cTOT 设备时相比，当 cTOT 设备开启时，可观察到 pelA、pcrV 和 acpP 的转录上调，它们分别负责细胞内粘附、3 型分泌系统的针尖蛋白生产和增殖过程中的脂肪酸合成。数据表明，经过 cTOT 治疗后，细菌细胞内的新陈代谢活动有所增加。此前已有研究表明，氧气可通过加强新陈代谢提高生物膜对抗生素的敏感性。观察到的基因表达变化突显了 cTOT 对生物膜的影响，可能会影响伤口抗菌治疗的成功率。有必要进一步开展体外和临床研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of wound care DERMATOLOGY-

CiteScore

2.90

自引率

10.50%

发文量

215

期刊介绍： Journal of Wound Care (JWC) is the definitive wound-care journal and the leading source of up-to-date research and clinical information on everything related to tissue viability. The journal was first launched in 1992 and aimed at catering to the needs of the multidisciplinary team. Published monthly, the journal’s international audience includes nurses, doctors and researchers specialising in wound management and tissue viability, as well as generalists wishing to enhance their practice. In addition to cutting edge and state-of-the-art research and practice articles, JWC also covers topics related to wound-care management, education and novel therapies, as well as JWC cases supplements, a supplement dedicated solely to case reports and case series in wound care. All articles are rigorously peer-reviewed by a panel of international experts, comprised of clinicians, nurses and researchers. Specifically, JWC publishes: High quality evidence on all aspects of wound care, including leg ulcers, pressure ulcers, the diabetic foot, burns, surgical wounds, wound infection and more The latest developments and innovations in wound care through both preclinical and preliminary clinical trials of potential new treatments worldwide In-depth prospective studies of new treatment applications, as well as high-level research evidence on existing treatments Clinical case studies providing information on how to deal with complex wounds Comprehensive literature reviews on current concepts and practice, including cost-effectiveness Updates on the activities of wound care societies around the world.