Circulation of influenza viruses in the dog population in Kazakhstan (2023-2024).

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.17

Tatyana I Glebova, Nailya G Klivleyeva, Nurbol T Saktaganov, Mira G Shamenova, Galina V Lukmanova, Assem M Baimukhametova, Sagadat B Baiseiit, Nuray S Ongarbayeva, Kanat A Orynkhanov, Anna V Ametova, Aitolkyn K Ilicheva

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Abstract

Background: Dogs in close contact with humans can serve as a source of potentially dangerous reassortant influenza viruses (IVs) with zoonotic potential. The dog's body can serve as a vessel for the emergence of new IVs. These new viruses can become a source of infection for other animals and humans. The potential for zoonotic transmission of IVs from dogs to humans poses a public health risk.

Aim: Study of the circulation of IVs in the dog population in Almaty, Kazakhstan.

Methods: Biosamples (oropharyngeal swabs and blood serum) from dogs were collected from veterinary clinics in Almaty in 2023-2024. Samples were screened using RT-PCR, HI assay, and ELISA.

Results: RT-PCR analysis of 355 nasopharyngeal swabs showed the presence of influenza A virus (IAV) in 32 samples (9.01% of the total number of samples analyzed). When subtyping IAV H1N1 RNA was detected in 19 swabs (5.35%). IAV subtype could not be determined in 13 PCR-positive samples (3.66%). The genetic material of IAV H3N2, H5, H7, and H9, as well as coronavirus, bocavirus, and adenovirus has not been identified. In a serological analysis of 180 blood sera using ELISA, antibodies to IAV were detected in 5.56% (n = 10). The results of the HI assay showed the presence of antihemagglutinins to A/H1N1pdm in 6.11% (11 samples), to A/H3N2 in 9.44% (17 samples), and no antibodies to IAV H5, H7, and type B were detected.

Conclusion: There is no information about human infection with any canine influenza virus. However, many cases of infection in dogs with human IAVs H1N1, H1N1pdm09, and H3N2 have been described. When dogs are co-infected with different IAVs, new recombinant IAVs may emerge that can infect humans and other animals. Therefore, ongoing global surveillance of animal populations is necessary to monitor the evolution and circulation of viruses dangerous to public health. This is also important for timely preparation for the emergence of a new zoonotic influenza virus that has pandemic potential for humans.

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哈萨克斯坦狗群中流感病毒的流行情况（2023-2024 年）。

背景：与人类密切接触的狗可能成为具有人畜共患病潜能的潜在危险流感病毒（IV）的来源。狗的身体可以成为新的 IV 病毒出现的容器。这些新病毒会成为其他动物和人类的传染源。人畜共患病病毒从狗传染给人类的可能性对公共健康构成威胁：方法：2023-2024 年在阿拉木图的兽医诊所收集了狗的生物样本（口咽拭子和血清）。使用 RT-PCR、HI 检测法和 ELISA 对样本进行筛查：对 355 份鼻咽拭子进行的 RT-PCR 分析表明，32 份样本（占分析样本总数的 9.01%）存在甲型流感病毒（IAV）。在对 19 个样本（5.35%）进行亚型鉴定时，检测到了 IAV H1N1 RNA。13 份 PCR 阳性样本（3.66%）中的 IAV 亚型无法确定。IAV H3N2、H5、H7 和 H9 以及冠状病毒、轮状病毒和腺病毒的遗传物质尚未确定。在使用 ELISA 对 180 份血清进行的血清学分析中，5.56%（n = 10）的血清中检测到了 IAV 抗体。HI 检测结果显示，6.11%（11 个样本）和 9.44%（17 个样本）的血清中存在抗 A/H1N1pdm、抗 A/H3N2、抗 H5、H7 和 B 型 IAV 血凝素抗体：结论：目前尚无人类感染任何犬流感病毒的信息。结论：目前还没有关于人类感染任何犬流感病毒的信息，但有许多犬感染人类 IAV H1N1、H1N1pdm09 和 H3N2 的病例。当狗同时感染不同的 IAV 时，可能会出现新的重组 IAV，从而感染人类和其他动物。因此，有必要对动物种群进行持续的全球监控，以监测危害公共健康的病毒的演变和流通情况。这对于及时防备可能对人类造成大流行的新型人畜共患流感病毒的出现也很重要。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.