首页 > 最新文献

Open Veterinary Journal最新文献

英文 中文
Monitoring the prevalence of Pseudomonas fluorescens as a spoilage indicator in cow raw milk, teat surfaces, and milk tanks. 监测作为奶牛生乳、乳头表面和储奶罐中腐败指标的荧光假单胞菌的流行情况。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.27
Ahmed Hamdi Ahmed, Muntaha Ghazi Hassan

Background: Milk and its products are very sensitive to spoilage if they are kept under unsuitable conditions which may provide favorable circumstances for the growth of specific spoilage organisms, Pseudomonas fluorescens accounted as the most dominant indicator for milk spoilage.

Aim: This study highlights monitoring the prevalence of P. fluorescens as a spoilage indicator organism in cow raw milk and its contact surfaces represented by teat surfaces and milk tanks in Nineveh province.

Methods: A total of 150 samples from cows' raw milk, teat surfaces, and milk tank swabs were collected from different locations in Nineveh province from October 2023 till February 2024. The Pseudomonas fluorescens were detected by using conventional cultivation methods supported by molecular detection of the target pathogen using the polymerase chain reaction technique.

Results: Out of 150 samples, 48 (32%) were positive for the prevalence of P. fluorescens by traditional methods, and 39 (26%) were positive using PCR assay according to the 16SPflu gene yielded a band at 850 bp. The P. fluorescens was recovered at 19 (38%) from raw milk. Teat surfaces revealed a higher isolation rate 11 (22%) compared to milk tanks 9 (18%). The mean counts of Pseudomonas in cows raw milk revealed 4.38, 6.29, and 7.37 log CFU/ml for the 0, 3, and 6 days of storage at chilling temperature. Results of DNA sequencing of the 16SrRNA gene revealed 12 strains recorded in the GenBank nucleotide sequence database.

Conclusion: Our results shed light on the risk of P. fluorescens prevalence as a spoilage indicator in raw milk and surrounding surfaces which is inevitable to apply hygienic procedures during milk collecting, processing, and preservation to increase the shelf life of the products and ensure milk safety and consumer health.

背景:目的:本研究重点监测尼尼微省奶牛生乳及其接触表面(以乳头表面和奶罐为代表)中作为腐败指示菌的荧光假单胞菌的流行情况:方法:从 2023 年 10 月到 2024 年 2 月,从尼尼微省的不同地点共采集了 150 份奶牛生乳、乳头表面和奶罐拭子样本。采用传统的培养方法检测荧光假单胞菌,并利用聚合酶链反应技术对目标病原体进行分子检测:结果:在 150 份样本中,48 份(32%)通过传统方法检测出荧光假单胞菌阳性,39 份(26%)通过聚合酶链反应检测出 16SPflu 基因阳性,该基因可产生 850 bp 的条带。有 19 个(38%)从生牛奶中检出了荧光假丝酵母菌。乳头表面的分离率为 11(22%),而奶槽的分离率为 9(18%)。冷藏 0、3 和 6 天后,奶牛生奶中假单胞菌的平均计数分别为 4.38、6.29 和 7.37 log CFU/ml。16SrRNA 基因的 DNA 测序结果显示,GenBank 核苷酸序列数据库中记录了 12 个菌株:我们的研究结果揭示了荧光荚膜杆菌作为一种腐败指标存在于原料奶及其周围表面的风险,因此在牛奶收集、加工和保存过程中必须采用卫生程序,以延长产品的保质期,确保牛奶安全和消费者健康。
{"title":"Monitoring the prevalence of <i>Pseudomonas fluorescens</i> as a spoilage indicator in cow raw milk, teat surfaces, and milk tanks.","authors":"Ahmed Hamdi Ahmed, Muntaha Ghazi Hassan","doi":"10.5455/OVJ.2024.v14.i8.27","DOIUrl":"10.5455/OVJ.2024.v14.i8.27","url":null,"abstract":"<p><strong>Background: </strong>Milk and its products are very sensitive to spoilage if they are kept under unsuitable conditions which may provide favorable circumstances for the growth of specific spoilage organisms, <i>Pseudomonas fluorescens</i> accounted as the most dominant indicator for milk spoilage.</p><p><strong>Aim: </strong>This study highlights monitoring the prevalence of <i>P. fluorescens</i> as a spoilage indicator organism in cow raw milk and its contact surfaces represented by teat surfaces and milk tanks in Nineveh province.</p><p><strong>Methods: </strong>A total of 150 samples from cows' raw milk, teat surfaces, and milk tank swabs were collected from different locations in Nineveh province from October 2023 till February 2024. The <i>Pseudomonas fluorescens</i> were detected by using conventional cultivation methods supported by molecular detection of the target pathogen using the polymerase chain reaction technique.</p><p><strong>Results: </strong>Out of 150 samples, 48 (32%) were positive for the prevalence of <i>P. fluorescens</i> by traditional methods, and 39 (26%) were positive using PCR assay according to the <i>16SPflu</i> gene yielded a band at 850 bp. The <i>P. fluorescens</i> was recovered at 19 (38%) from raw milk. Teat surfaces revealed a higher isolation rate 11 (22%) compared to milk tanks 9 (18%). The mean counts of <i>Pseudomonas</i> in cows raw milk revealed 4.38, 6.29, and 7.37 log CFU/ml for the 0, 3, and 6 days of storage at chilling temperature. Results of DNA sequencing of the <i>16SrRNA</i> gene revealed 12 strains recorded in the GenBank nucleotide sequence database.</p><p><strong>Conclusion: </strong>Our results shed light on the risk of <i>P. fluorescens</i> prevalence as a spoilage indicator in raw milk and surrounding surfaces which is inevitable to apply hygienic procedures during milk collecting, processing, and preservation to increase the shelf life of the products and ensure milk safety and consumer health.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The adverse side effects of prenatal and postnatal rats' exposure to silver nanoparticles Induced toxicity. 大鼠产前和产后暴露于纳米银粒子诱导毒性的不良副作用。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.29
Sultan Al-Haid, Mahmoud Elalfy, Eman Alsyaed, Mamdouh Abouelmagd, Ahmad Al-Jazzar, Fahad A Al-Hizab, Wageh Sobhy Darwish, AbdelRahman Hereba, Mona Elhadidy

Background: Silver nanotechnology is widely applied in industry and medicine, with an increased likelihood of environmental and food contamination.

Aim: This study aimed to explore the adverse effects of orally administering silver nanoparticles (AgNPs) to pregnant or lactating female rats on adults and the development of their offspring.

Methods: Forty female albino rats were used to assess the immediate impacts of AgNPs in two separate experiments. The experimental group received 1 ml of AgNPs, dissolved in deionized water, at doses of 0, 50, and 100 mg/kg of body weight from the 6th to the 15th day of gestation in pregnant albino rats. After a 20-day gestation period, euthanasia was performed on the female rats, followed by a gross examination post-dissection.

Results: The feti were preserved in ethyl alcohol and Poin's solution for the identification of skeletal and visceral malformations. It was noticed that feti of dams that received AgNPs showed teratogenicities such as delayed ossification and deletion of bones or ribs. Notably, dams showed necrosis and satellitosis with evidence of behavioral alteration. While rats' pups showed only brain edema and no behavioral changes.

Conclusion: AgNPs at a dose of 50 or 100 mg/kg induced teratogenic effect in terms of delayed ossification, abnormal limb formation, and brain edema in rat pups, however, induced necrosis and satellitosis in dam rats. Hence, greater emphasis should be placed on preventing exposure to Ag-NPs, especially among pregnant females.

背景:目的:本研究旨在探讨怀孕或哺乳期雌性大鼠口服纳米银颗粒(AgNPs)对成年大鼠及其后代发育的不良影响:40只雌性白化大鼠分别在两个实验中评估了AgNPs的直接影响。实验组在怀孕白化大鼠妊娠期的第 6 天至第 15 天按每公斤体重 0、50 和 100 毫克的剂量,分别摄入 1 毫升溶于去离子水的 AgNPs。妊娠 20 天后,对雌性大鼠实施安乐死,然后进行解剖后的大体检查:结果:胎儿被保存在乙醇和波因氏溶液中,用于鉴定骨骼和内脏畸形。结果发现,摄入 AgNPs 的母鼠胎儿有致畸现象,如骨化延迟、骨骼或肋骨缺失。值得注意的是,母鼠的胎儿出现坏死和坐骨神经萎缩,并有行为改变的迹象。大鼠幼崽仅出现脑水肿,行为无变化:结论:剂量为 50 或 100 毫克/千克的 AgNPs 会诱发致畸效应,表现为大鼠幼崽骨化延迟、肢体形成异常和脑水肿,但会诱发大鼠母体坏死和坐骨神经萎缩。因此,应更加重视防止接触 Ag-NPs,尤其是怀孕雌鼠。
{"title":"The adverse side effects of prenatal and postnatal rats' exposure to silver nanoparticles Induced toxicity.","authors":"Sultan Al-Haid, Mahmoud Elalfy, Eman Alsyaed, Mamdouh Abouelmagd, Ahmad Al-Jazzar, Fahad A Al-Hizab, Wageh Sobhy Darwish, AbdelRahman Hereba, Mona Elhadidy","doi":"10.5455/OVJ.2024.v14.i8.29","DOIUrl":"10.5455/OVJ.2024.v14.i8.29","url":null,"abstract":"<p><strong>Background: </strong>Silver nanotechnology is widely applied in industry and medicine, with an increased likelihood of environmental and food contamination.</p><p><strong>Aim: </strong>This study aimed to explore the adverse effects of orally administering silver nanoparticles (AgNPs) to pregnant or lactating female rats on adults and the development of their offspring.</p><p><strong>Methods: </strong>Forty female albino rats were used to assess the immediate impacts of AgNPs in two separate experiments. The experimental group received 1 ml of AgNPs, dissolved in deionized water, at doses of 0, 50, and 100 mg/kg of body weight from the 6th to the 15th day of gestation in pregnant albino rats. After a 20-day gestation period, euthanasia was performed on the female rats, followed by a gross examination post-dissection.</p><p><strong>Results: </strong>The feti were preserved in ethyl alcohol and Poin's solution for the identification of skeletal and visceral malformations. It was noticed that feti of dams that received AgNPs showed teratogenicities such as delayed ossification and deletion of bones or ribs. Notably, dams showed necrosis and satellitosis with evidence of behavioral alteration. While rats' pups showed only brain edema and no behavioral changes.</p><p><strong>Conclusion: </strong>AgNPs at a dose of 50 or 100 mg/kg induced teratogenic effect in terms of delayed ossification, abnormal limb formation, and brain edema in rat pups, however, induced necrosis and satellitosis in dam rats. Hence, greater emphasis should be placed on preventing exposure to Ag-NPs, especially among pregnant females.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of four different protocols using levobupivacaine for spinal anaesthesia in red-footed tortoises (Chelonoidis carbonarius). 使用左旋布比卡因对红脚陆龟(Chelonoidis carbonarius)进行脊髓麻醉的四种不同方案的比较。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.6
Paulo Cesar Mendes Dos Santos Filho, Leticia Prata Juliano Dimatteu Telles, Elane Barboza da Silva, Dandara Franco Ferreira da Silva, Líria Queiroz Luz Hirano

Background: The popularity of tortoises kept in captivity is increasing and has caused concern regarding the necessity to establish safe and straightforward anaesthesia for those reptiles.

Aim: This study aimed to compare four protocols using levobupivacaine in spinal anaesthesia for the blockade of the caudal neuraxis of red-footed tortoises (Chelonoidis carbonarius).

Methods: Twenty-four tortoises were randomly assigned into four groups: G1, levobupivacaine 0.75% (1.15 mg kg-1); G2, levobupivacaine 0.37% (1.15 mg kg-1); G3, levobupivacaine 0.75% (2.3 mg kg-1); and G4, levobupivacaine 0.75% (0.1 ml 5 cm-1 of straight carapace length). Tortoises were evaluated for respiratory rate, muscle relaxation, response to hindlimb or tail pinch, and cloacal reflex.

Results: A 1.15 mg kg-1 dose of levobupivacaine 0.37% appears adequate for shorter procedures, whereas a 1.15 mg kg-1 dose of levobupivacaine 0.75% should be appropriate for longer procedures in red-footed tortoises.

Conclusion: Our results are the first to show the effects of levobupivacaine on spinal anaesthesia in reptiles. Weight-based doses presented more intense and more homogeneous effects than carapace length-based doses in red-footed tortoises. Spinal anaesthesia of red-footed tortoises was safe and effective with any of the weight-based protocols.

背景:目的:本研究旨在比较在脊髓麻醉中使用左旋布比卡因阻滞红脚陆龟(Chelonoidis carbonarius)尾部神经轴的四种方案:方法:将 24 只陆龟随机分为四组:G1,左旋布比卡因 0.75% (1.15 mg kg-1);G2,左旋布比卡因 0.37% (1.15 mg kg-1);G3,左旋布比卡因 0.75% (2.3 mg kg-1);G4,左旋布比卡因 0.75% (0.1 ml 5 cm-1 直躯干长度)。对陆龟的呼吸频率、肌肉松弛、对后肢或尾部挤压的反应以及泄殖腔反射进行评估:结果:1.15 毫克/千克-1 的左旋布比卡因 0.37% 剂量似乎适合较短的手术,而 1.15 毫克/千克-1 的左旋布比卡因 0.75% 剂量应适合较长的红脚陆龟手术:我们的研究结果首次显示了左旋布比卡因对爬行动物脊髓麻醉的影响。在红脚陆龟身上,基于体重的剂量比基于躯干长度的剂量产生的效果更强烈、更均匀。使用任何一种基于体重的方案对红脚陆龟进行脊髓麻醉都是安全有效的。
{"title":"Comparison of four different protocols using levobupivacaine for spinal anaesthesia in red-footed tortoises (<i>Chelonoidis carbonarius</i>).","authors":"Paulo Cesar Mendes Dos Santos Filho, Leticia Prata Juliano Dimatteu Telles, Elane Barboza da Silva, Dandara Franco Ferreira da Silva, Líria Queiroz Luz Hirano","doi":"10.5455/OVJ.2024.v14.i8.6","DOIUrl":"10.5455/OVJ.2024.v14.i8.6","url":null,"abstract":"<p><strong>Background: </strong>The popularity of tortoises kept in captivity is increasing and has caused concern regarding the necessity to establish safe and straightforward anaesthesia for those reptiles.</p><p><strong>Aim: </strong>This study aimed to compare four protocols using levobupivacaine in spinal anaesthesia for the blockade of the caudal neuraxis of red-footed tortoises (<i>Chelonoidis carbonarius</i>).</p><p><strong>Methods: </strong>Twenty-four tortoises were randomly assigned into four groups: G1, levobupivacaine 0.75% (1.15 mg kg<sup>-1</sup>); G2, levobupivacaine 0.37% (1.15 mg kg<sup>-1</sup>); G3, levobupivacaine 0.75% (2.3 mg kg<sup>-1</sup>); and G4, levobupivacaine 0.75% (0.1 ml 5 cm<sup>-1</sup> of straight carapace length). Tortoises were evaluated for respiratory rate, muscle relaxation, response to hindlimb or tail pinch, and cloacal reflex.</p><p><strong>Results: </strong>A 1.15 mg kg<sup>-1</sup> dose of levobupivacaine 0.37% appears adequate for shorter procedures, whereas a 1.15 mg kg<sup>-1</sup> dose of levobupivacaine 0.75% should be appropriate for longer procedures in red-footed tortoises.</p><p><strong>Conclusion: </strong>Our results are the first to show the effects of levobupivacaine on spinal anaesthesia in reptiles. Weight-based doses presented more intense and more homogeneous effects than carapace length-based doses in red-footed tortoises. Spinal anaesthesia of red-footed tortoises was safe and effective with any of the weight-based protocols.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics. hCG 和前列腺素对亚热带炎热夏季胚胎供体母马卵巢、黄体发育和激素变化的影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.35
Jamal Mohamed Hassan Alkhadrawy, Amal Mahmoud Aboelmaaty, Mostafa Mohamed Abou-Ahmed, Abdelraouf Morsy Ghallab

Background: Commercial embryo flushing of horses has required hormonal management of both the donor and recipient mares throughout the breeding season.

Aim: This study aimed to find out the effect of using human chorionic gonadotropin (hCG) and prostaglandin F2α (PG) on the ovarian and uterine dynamics and hemodynamics, estradiol (E2), progesterone, oxidants-antioxidants, and blood biochemicals in embryo donor mares during the hottest months of the year in a subtropical climate.

Methods: Three Control estrous cycles of native mares (10-20 years; N = 10) followed by two treated cycles with hCG and PGF2α were examined daily from May to August using Doppler ultrasound with blood sampling. Circulating, progesterone (P4), total cholesterol, total proteins, albumin, haptoglobin, nitric oxide (NO), catalase, alkaline phosphatase, lactate dehydrogenase (LDH), and myeloperoxidase were measured in blood serum.

Results: Days during the control estrous cycle impacted the dominant follicle (DF) diameter ( p < 0.0001), antrum diameter ( p < 0.0001), area ( p < 0.0001), antral area ( p < 0.0001), and color area % (p > 0.05), and corpus luteum (CL) diameter ( p < 0.0001). PG tended to impact DF diameter (p > 0.05) but influenced its antrum diameter (p < 0.05), color area (p < 0.05), CL diameter (p < 0.01), and area (p = 0.013). Days after hCG tended to impact DF antrum diameter (p > 0.05) and the antrum area (p > 0.05), but influenced CL diameter ( p < 0.0001). PGF2α and hCG increased uterine horn area (p = 0.016) and color area (p = 0.023), total cholesterol ( p < 0.0001), and NO ( p < 0.0001) levels but hCG increased the levels of myeloperoxidase (p < 0.005), total proteins (p < 0.001), and albumin ( p < 0.0001). Globulins achieved the highest level (p = 0.054) but the Albumin/globulin ratio reached a minimum value on Day 0 of the control mares ( p < 0.0001). PGF2α increased LDH ( p < 0.0001) and sharply declined (p = 0.028) progesterone.

Conclusion: In conclusion, the treatment protocols of hCG and PGF2α showed minimal effects on the produced ovulating follicles and can be used during the summer season to manage embryo donor mares.

背景:马匹的商业胚胎冲洗需要在整个繁殖季节对供体母马和受体母马进行激素管理。目的:本研究旨在了解在亚热带气候一年中最炎热的月份使用人绒毛膜促性腺激素(hCG)和前列腺素 F2α (PG)对胚胎供体母马的卵巢和子宫动态及血液动力学、雌二醇(E2)、孕酮、氧化剂-抗氧化剂和血液生化指标的影响:从 5 月到 8 月,每天使用多普勒超声波和血液采样对本地母马(10-20 岁;N = 10)的三个控制发情周期和两个使用 hCG 和 PGF2α 的处理周期进行检查。测量血清中的循环、孕酮(P4)、总胆固醇、总蛋白、白蛋白、血红蛋白、一氧化氮(NO)、过氧化氢酶、碱性磷酸酶、乳酸脱氢酶(LDH)和髓过氧化物酶:对照组发情周期的天数对优势卵泡(DF)直径(p < 0.0001)、窦直径(p < 0.0001)、面积(p < 0.0001)、窦面积(p < 0.0001)和着色面积%(p > 0.05)以及黄体(CL)直径(p < 0.0001)有影响。PG 往往影响 DF 直径(p > 0.05),但影响其窦道直径(p < 0.05)、着色面积(p < 0.05)、CL 直径(p < 0.01)和面积(p = 0.013)。hCG 后的天数往往影响 DF 窦直径(p > 0.05)和窦面积(p > 0.05),但影响 CL 直径(p < 0.0001)。PGF2α 和 hCG 增加了子宫角面积(p = 0.016)和颜色面积(p = 0.023)、总胆固醇(p < 0.0001)和 NO(p < 0.0001)水平,但 hCG 增加了骨髓过氧化物酶(p < 0.005)、总蛋白(p < 0.001)和白蛋白(p < 0.0001)水平。球蛋白达到最高水平(p = 0.054),但白蛋白/球蛋白比率在对照组母马的第 0 天达到最低值(p < 0.0001)。PGF2α使LDH升高(p < 0.0001),使孕酮急剧下降(p = 0.028):总之,hCG 和 PGF2α 的治疗方案对排卵卵泡的影响极小,可用于夏季胚胎捐献母马的管理。
{"title":"Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics.","authors":"Jamal Mohamed Hassan Alkhadrawy, Amal Mahmoud Aboelmaaty, Mostafa Mohamed Abou-Ahmed, Abdelraouf Morsy Ghallab","doi":"10.5455/OVJ.2024.v14.i8.35","DOIUrl":"10.5455/OVJ.2024.v14.i8.35","url":null,"abstract":"<p><strong>Background: </strong>Commercial embryo flushing of horses has required hormonal management of both the donor and recipient mares throughout the breeding season.</p><p><strong>Aim: </strong>This study aimed to find out the effect of using human chorionic gonadotropin (hCG) and prostaglandin F2α (PG) on the ovarian and uterine dynamics and hemodynamics, estradiol (E2), progesterone, oxidants-antioxidants, and blood biochemicals in embryo donor mares during the hottest months of the year in a subtropical climate.</p><p><strong>Methods: </strong>Three Control estrous cycles of native mares (10-20 years; <i>N</i> = 10) followed by two treated cycles with hCG and PGF2α were examined daily from May to August using Doppler ultrasound with blood sampling. Circulating, progesterone (P4), total cholesterol, total proteins, albumin, haptoglobin, nitric oxide (NO), catalase, alkaline phosphatase, lactate dehydrogenase (LDH), and myeloperoxidase were measured in blood serum.</p><p><strong>Results: </strong>Days during the control estrous cycle impacted the dominant follicle (DF) diameter ( <i>p</i> < 0.0001), antrum diameter ( <i>p</i> < 0.0001), area ( <i>p</i> < 0.0001), antral area ( <i>p</i> < 0.0001), and color area % (<i>p</i> > 0.05), and corpus luteum (CL) diameter ( <i>p</i> < 0.0001). PG tended to impact DF diameter (<i>p</i> > 0.05) but influenced its antrum diameter (<i>p</i> < 0.05), color area (<i>p</i> < 0.05), CL diameter (<i>p</i> < 0.01), and area (<i>p</i> = 0.013). Days after hCG tended to impact DF antrum diameter (<i>p</i> > 0.05) and the antrum area (<i>p</i> > 0.05), but influenced CL diameter ( <i>p</i> < 0.0001). PGF2α and hCG increased uterine horn area (<i>p</i> = 0.016) and color area (<i>p</i> = 0.023), total cholesterol ( <i>p</i> < 0.0001), and NO ( <i>p</i> < 0.0001) levels but hCG increased the levels of myeloperoxidase (<i>p</i> < 0.005), total proteins (<i>p</i> < 0.001), and albumin ( <i>p</i> < 0.0001). Globulins achieved the highest level (<i>p</i> = 0.054) but the Albumin/globulin ratio reached a minimum value on Day 0 of the control mares ( <i>p</i> < 0.0001). PGF2α increased LDH ( <i>p</i> < 0.0001) and sharply declined (<i>p</i> = 0.028) progesterone.</p><p><strong>Conclusion: </strong>In conclusion, the treatment protocols of hCG and PGF2α showed minimal effects on the produced ovulating follicles and can be used during the summer season to manage embryo donor mares.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial 16S rRNA gene as a molecular marker in the phylogenetic relationships of some Rabbitfishes species (Siganidae: Perciformes). 线粒体 16S rRNA 基因作为一些兔鱼(Siganidae: Perciformes)物种系统发育关系中的分子标记。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.22
Najiah M Alyamani

Background: Siganidae is a marine teleost family consisting of a single extant genus, Siganus Forsskål, 1775, which included 29 recognized species of rabbitfish.

Aim: The main goal of this study was the use of the mitochondrial 16S rRNA gene as a potential molecular marker in the phylogenetic relationships study of some rabbitfishes species (Siganidae: Perciformes).

Methods: The samples were gathered from the Red Sea. The sequences of four rabbitfishes (Siganus argenteus, Siganus luridus, Siganus rivulatus, and Siganus stellatus) were deposited into NCBI to gain the accession numbers (PP488874-PP488877) and then analyzed with their related rabbitfishes depending on available sequence data of the mitochondrial 16S rRNA gene.

Results: The results of 16S rRNA sequences illustrated that the average A+T values were greater than C+G.

Conclusion: The low genetic distance between S. luridus and Siganus rivulatus indicated a close linkage between them.

背景:目的:本研究的主要目的是利用线粒体 16S rRNA 基因作为潜在的分子标记,对一些兔形鱼(兔形鱼科:鲈形目)物种进行系统发育关系研究:方法:样品采集自红海。方法:样品取自红海,将四种兔鱼(Siganus argenteus、Siganus luridus、Siganus rivulatus 和 Siganus stellatus)的序列存入 NCBI,获得登录号(PP488874-PP488877),然后根据现有的线粒体 16S rRNA 基因序列数据,与相关兔鱼进行分析:16S rRNA序列结果表明,平均A+T值大于C+G值:结论:S. luridus 和 Siganus rivulatus 之间的遗传距离较小,表明它们之间存在密切的联系。
{"title":"Mitochondrial <i>16S rRNA</i> gene as a molecular marker in the phylogenetic relationships of some Rabbitfishes species (Siganidae: Perciformes).","authors":"Najiah M Alyamani","doi":"10.5455/OVJ.2024.v14.i8.22","DOIUrl":"10.5455/OVJ.2024.v14.i8.22","url":null,"abstract":"<p><strong>Background: </strong>Siganidae is a marine teleost family consisting of a single extant genus, <i>Siganus</i> Forsskål, 1775, which included 29 recognized species of rabbitfish.</p><p><strong>Aim: </strong>The main goal of this study was the use of the mitochondrial <i>16S rRNA</i> gene as a potential molecular marker in the phylogenetic relationships study of some rabbitfishes species (Siganidae: Perciformes).</p><p><strong>Methods: </strong>The samples were gathered from the Red Sea. The sequences of four rabbitfishes (<i>Siganus argenteus, Siganus luridus, Siganus rivulatus,</i> and <i>Siganus stellatus</i>) were deposited into NCBI to gain the accession numbers (PP488874-PP488877) and then analyzed with their related rabbitfishes depending on available sequence data of the mitochondrial <i>16S rRNA</i> gene.</p><p><strong>Results: </strong>The results of <i>16S rRNA</i> sequences illustrated that the average A+T values were greater than C+G.</p><p><strong>Conclusion: </strong>The low genetic distance between <i>S. luridus</i> and <i>Siganus rivulatus</i> indicated a close linkage between them.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular identification of Staphylococcus aureus-related enterotoxin genes in cheese samples. 奶酪样本中与金黄色葡萄球菌相关的肠毒素基因的分子鉴定。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.36
Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed

Background: Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.

Aim: The current study was carried out to identify if there is any presence of Staphylococcus aureus-related enterotoxin genes in cheese samples.

Methods: The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of icaA, sea, hla, and fnbA enterotoxin genes in these samples.

Results: The conventional identification revealed the growth of S. aureus from the cheese samples. The PCR findings recorded the presence of the icaA, sea, hla, and fnbA in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.

Conclusion: The current results indicate the presence of enterotoxin genes of S. aureus in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.

背景:乳制品被认为是各种营养成分的重要来源;然而,病原菌的生长是这些产品的一个关键破坏因素,导致消费者健康和系统财务危机。目的:本研究旨在确定奶酪样本中是否存在与金黄色葡萄球菌相关的肠毒素基因:研究包括收集 35 个样本。方法:研究包括收集 35 个样本,样本经过常规培养过程和 PCR 方法检测样本中是否存在 icaA、sea、hla 和fnbA 肠毒素基因:结果:常规鉴定显示,奶酪样本中生长了金黄色葡萄球菌。聚合酶链式反应结果显示,奶酪样本中分别有 31 个(88.5%)、27 个(77%)、19 个(54%)和 12 个(34%)存在 icaA、sea、hla 和 fnbA 基因。测序结果表明,与全球分离物的相似度高达 98.5%:结论:目前的研究结果表明,金黄色葡萄球菌的肠毒素基因在受检乳制品中的存在率很高,这揭示了由于消费者乳制品中可能存在肠毒素而导致的严重食品安全问题。
{"title":"Molecular identification of <i>Staphylococcus aureus</i>-related enterotoxin genes in cheese samples.","authors":"Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed","doi":"10.5455/OVJ.2024.v14.i8.36","DOIUrl":"10.5455/OVJ.2024.v14.i8.36","url":null,"abstract":"<p><strong>Background: </strong>Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.</p><p><strong>Aim: </strong>The current study was carried out to identify if there is any presence of <i>Staphylococcus aureus</i>-related enterotoxin genes in cheese samples.</p><p><strong>Methods: </strong>The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> enterotoxin genes in these samples.</p><p><strong>Results: </strong>The conventional identification revealed the growth of <i>S</i>. <i>aureus</i> from the cheese samples. The PCR findings recorded the presence of the <i>icaA</i>, <i>sea</i>, <i>hla</i>, and <i>fnbA</i> in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.</p><p><strong>Conclusion: </strong>The current results indicate the presence of enterotoxin genes of <i>S. aureus</i> in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Campylobacteriosis: A rising threat in foodborne illnesses. 弯曲杆菌病:食源性疾病中不断上升的威胁。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.1
Aswin Rafif Khairullah, Sheila Marty Yanestria, Mustofa Helmi Effendi, Ikechukwu Benjamin Moses, Muhammad Khaliim Jati Kusala, Kartika Afrida Fauzia, Siti Rani Ayuti, Ima Fauziah, Otto Sahat Martua Silaen, Katty Hendriana Priscilia Riwu, Suhita Aryaloka, Fidi Nur Aini Eka Puji Dameanti, Ricadonna Raissa, Abdullah Hasib, Abdul Hadi Furqoni

Campylobacteriosis is a foodborne illness that is contracted by eating contaminated food, particularly animal products like meat from diseased animals or corpses tainted with harmful germs. The epidemiology of campylobacteriosis varies significantly between low-, middle-, and high-income countries. Campylobacter has a complicated and poorly known survival strategy for getting past host barriers and causing sickness in humans. The adaptability of Campylobacter to unfavorable environments and the host's immune system seems to be one of the most crucial elements of intestinal colonization. A Campylobacter infection may result in fever, nausea, vomiting, and mild to severe bloody diarrhea in humans. Effective and rapid diagnosis of Campylobacter species infections in animal hosts is essential for both individual treatment and disease management at the farm level. According to the most recent meta-analysis research, the main risk factor for campylobacteriosis is travel, which is followed by eating undercooked chicken, being exposed to the environment, and coming into close contact with livestock. Campylobacter jejuni, and occasionally Campylobacter coli, are the primary causes of Campylobacter gastroenteritis, the most significant Campylobacter infection in humans for public health. The best antibiotic medications for eradicating and decreasing Campylobacter in feces are erythromycin, clarithromycin, or azithromycin. The best strategy to reduce the number of human infections caused by Campylobacter is to restrict the amount of contamination of the poultry flock and its products, even if the majority of infections are contracted through handling or ingestion of chicken.

弯曲杆菌病是一种食源性疾病,通过食用受污染的食物而感染,尤其是动物产品,如病死动物的肉或沾染有害病菌的尸体。弯曲杆菌病的流行病学在低收入、中等收入和高收入国家之间存在很大差异。弯曲杆菌的生存策略非常复杂,而且鲜为人知,它可以穿过宿主的屏障,导致人类患病。弯曲杆菌对不利环境和宿主免疫系统的适应能力似乎是肠道定植的最关键因素之一。弯曲杆菌感染可能导致人类发烧、恶心、呕吐和轻度至重度血性腹泻。有效、快速地诊断动物宿主的弯曲杆菌感染对于个体治疗和农场层面的疾病管理都至关重要。根据最新的荟萃分析研究,弯曲杆菌病的主要风险因素是旅行,其次是食用未煮熟的鸡肉、暴露于环境中以及与牲畜密切接触。空肠弯曲菌,偶尔还有大肠弯曲菌,是弯曲菌胃肠炎的主要病因,也是对公共卫生影响最大的人类弯曲菌感染。根除和减少粪便中弯曲杆菌的最佳抗生素药物是红霉素、克拉霉素或阿奇霉素。减少由弯曲杆菌引起的人类感染的最佳策略是限制禽群及其产品的污染量,即使大多数感染是通过处理或摄入鸡肉感染的。
{"title":"Campylobacteriosis: A rising threat in foodborne illnesses.","authors":"Aswin Rafif Khairullah, Sheila Marty Yanestria, Mustofa Helmi Effendi, Ikechukwu Benjamin Moses, Muhammad Khaliim Jati Kusala, Kartika Afrida Fauzia, Siti Rani Ayuti, Ima Fauziah, Otto Sahat Martua Silaen, Katty Hendriana Priscilia Riwu, Suhita Aryaloka, Fidi Nur Aini Eka Puji Dameanti, Ricadonna Raissa, Abdullah Hasib, Abdul Hadi Furqoni","doi":"10.5455/OVJ.2024.v14.i8.1","DOIUrl":"10.5455/OVJ.2024.v14.i8.1","url":null,"abstract":"<p><p>Campylobacteriosis is a foodborne illness that is contracted by eating contaminated food, particularly animal products like meat from diseased animals or corpses tainted with harmful germs. The epidemiology of campylobacteriosis varies significantly between low-, middle-, and high-income countries. Campylobacter has a complicated and poorly known survival strategy for getting past host barriers and causing sickness in humans. The adaptability of <i>Campylobacter</i> to unfavorable environments and the host's immune system seems to be one of the most crucial elements of intestinal colonization. A <i>Campylobacter</i> infection may result in fever, nausea, vomiting, and mild to severe bloody diarrhea in humans. Effective and rapid diagnosis of <i>Campylobacter</i> species infections in animal hosts is essential for both individual treatment and disease management at the farm level. According to the most recent meta-analysis research, the main risk factor for campylobacteriosis is travel, which is followed by eating undercooked chicken, being exposed to the environment, and coming into close contact with livestock. <i>Campylobacter jejuni</i>, and occasionally <i>Campylobacter coli</i>, are the primary causes of <i>Campylobacter</i> gastroenteritis, the most significant <i>Campylobacter</i> infection in humans for public health. The best antibiotic medications for eradicating and decreasing <i>Campylobacter</i> in feces are erythromycin, clarithromycin, or azithromycin. The best strategy to reduce the number of human infections caused by <i>Campylobacter</i> is to restrict the amount of contamination of the poultry flock and its products, even if the majority of infections are contracted through handling or ingestion of chicken.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Circulation of influenza viruses in the dog population in Kazakhstan (2023-2024). 哈萨克斯坦狗群中流感病毒的流行情况(2023-2024 年)。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.17
Tatyana I Glebova, Nailya G Klivleyeva, Nurbol T Saktaganov, Mira G Shamenova, Galina V Lukmanova, Assem M Baimukhametova, Sagadat B Baiseiit, Nuray S Ongarbayeva, Kanat A Orynkhanov, Anna V Ametova, Aitolkyn K Ilicheva

Background: Dogs in close contact with humans can serve as a source of potentially dangerous reassortant influenza viruses (IVs) with zoonotic potential. The dog's body can serve as a vessel for the emergence of new IVs. These new viruses can become a source of infection for other animals and humans. The potential for zoonotic transmission of IVs from dogs to humans poses a public health risk.

Aim: Study of the circulation of IVs in the dog population in Almaty, Kazakhstan.

Methods: Biosamples (oropharyngeal swabs and blood serum) from dogs were collected from veterinary clinics in Almaty in 2023-2024. Samples were screened using RT-PCR, HI assay, and ELISA.

Results: RT-PCR analysis of 355 nasopharyngeal swabs showed the presence of influenza A virus (IAV) in 32 samples (9.01% of the total number of samples analyzed). When subtyping IAV H1N1 RNA was detected in 19 swabs (5.35%). IAV subtype could not be determined in 13 PCR-positive samples (3.66%). The genetic material of IAV H3N2, H5, H7, and H9, as well as coronavirus, bocavirus, and adenovirus has not been identified. In a serological analysis of 180 blood sera using ELISA, antibodies to IAV were detected in 5.56% (n = 10). The results of the HI assay showed the presence of antihemagglutinins to A/H1N1pdm in 6.11% (11 samples), to A/H3N2 in 9.44% (17 samples), and no antibodies to IAV H5, H7, and type B were detected.

Conclusion: There is no information about human infection with any canine influenza virus. However, many cases of infection in dogs with human IAVs H1N1, H1N1pdm09, and H3N2 have been described. When dogs are co-infected with different IAVs, new recombinant IAVs may emerge that can infect humans and other animals. Therefore, ongoing global surveillance of animal populations is necessary to monitor the evolution and circulation of viruses dangerous to public health. This is also important for timely preparation for the emergence of a new zoonotic influenza virus that has pandemic potential for humans.

背景:与人类密切接触的狗可能成为具有人畜共患病潜能的潜在危险流感病毒(IV)的来源。狗的身体可以成为新的 IV 病毒出现的容器。这些新病毒会成为其他动物和人类的传染源。人畜共患病病毒从狗传染给人类的可能性对公共健康构成威胁:方法:2023-2024 年在阿拉木图的兽医诊所收集了狗的生物样本(口咽拭子和血清)。使用 RT-PCR、HI 检测法和 ELISA 对样本进行筛查:对 355 份鼻咽拭子进行的 RT-PCR 分析表明,32 份样本(占分析样本总数的 9.01%)存在甲型流感病毒(IAV)。在对 19 个样本(5.35%)进行亚型鉴定时,检测到了 IAV H1N1 RNA。13 份 PCR 阳性样本(3.66%)中的 IAV 亚型无法确定。IAV H3N2、H5、H7 和 H9 以及冠状病毒、轮状病毒和腺病毒的遗传物质尚未确定。在使用 ELISA 对 180 份血清进行的血清学分析中,5.56%(n = 10)的血清中检测到了 IAV 抗体。HI 检测结果显示,6.11%(11 个样本)和 9.44%(17 个样本)的血清中存在抗 A/H1N1pdm、抗 A/H3N2、抗 H5、H7 和 B 型 IAV 血凝素抗体:结论:目前尚无人类感染任何犬流感病毒的信息。结论:目前还没有关于人类感染任何犬流感病毒的信息,但有许多犬感染人类 IAV H1N1、H1N1pdm09 和 H3N2 的病例。当狗同时感染不同的 IAV 时,可能会出现新的重组 IAV,从而感染人类和其他动物。因此,有必要对动物种群进行持续的全球监控,以监测危害公共健康的病毒的演变和流通情况。这对于及时防备可能对人类造成大流行的新型人畜共患流感病毒的出现也很重要。
{"title":"Circulation of influenza viruses in the dog population in Kazakhstan (2023-2024).","authors":"Tatyana I Glebova, Nailya G Klivleyeva, Nurbol T Saktaganov, Mira G Shamenova, Galina V Lukmanova, Assem M Baimukhametova, Sagadat B Baiseiit, Nuray S Ongarbayeva, Kanat A Orynkhanov, Anna V Ametova, Aitolkyn K Ilicheva","doi":"10.5455/OVJ.2024.v14.i8.17","DOIUrl":"10.5455/OVJ.2024.v14.i8.17","url":null,"abstract":"<p><strong>Background: </strong>Dogs in close contact with humans can serve as a source of potentially dangerous reassortant influenza viruses (IVs) with zoonotic potential. The dog's body can serve as a vessel for the emergence of new IVs. These new viruses can become a source of infection for other animals and humans. The potential for zoonotic transmission of IVs from dogs to humans poses a public health risk.</p><p><strong>Aim: </strong>Study of the circulation of IVs in the dog population in Almaty, Kazakhstan.</p><p><strong>Methods: </strong>Biosamples (oropharyngeal swabs and blood serum) from dogs were collected from veterinary clinics in Almaty in 2023-2024. Samples were screened using RT-PCR, HI assay, and ELISA.</p><p><strong>Results: </strong>RT-PCR analysis of 355 nasopharyngeal swabs showed the presence of influenza A virus (IAV) in 32 samples (9.01% of the total number of samples analyzed). When subtyping IAV H1N1 RNA was detected in 19 swabs (5.35%). IAV subtype could not be determined in 13 PCR-positive samples (3.66%). The genetic material of IAV H3N2, H5, H7, and H9, as well as coronavirus, bocavirus, and adenovirus has not been identified. In a serological analysis of 180 blood sera using ELISA, antibodies to IAV were detected in 5.56% (<i>n</i> = 10). The results of the HI assay showed the presence of antihemagglutinins to A/H1N1pdm in 6.11% (11 samples), to A/H3N2 in 9.44% (17 samples), and no antibodies to IAV H5, H7, and type B were detected.</p><p><strong>Conclusion: </strong>There is no information about human infection with any canine influenza virus. However, many cases of infection in dogs with human IAVs H1N1, H1N1pdm09, and H3N2 have been described. When dogs are co-infected with different IAVs, new recombinant IAVs may emerge that can infect humans and other animals. Therefore, ongoing global surveillance of animal populations is necessary to monitor the evolution and circulation of viruses dangerous to public health. This is also important for timely preparation for the emergence of a new zoonotic influenza virus that has pandemic potential for humans.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathological and immunohistochemical studies of lymphoid leukosis in pigeons in Egypt. 埃及鸽子淋巴白血病的病理和免疫组织化学研究。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.24
Abdelmenem Ahmed Elmeligy, Ali A Ghania, Ahmed Fotouh

Background: Pigeon leukosis is primarily caused by avian leukosis virus subgroup A (ALV-A). It infects and transforms lymphoid cells, leading to the development of tumors in various lymphoid tissues and other organs especially the liver.

Aim: This study was conducted to diagnose lymphoid leukosis in a naturally infected pigeon flock in Egypt.

Methods: Tissue specimens from the liver, spleen, thymus, kidney, lung, proventriculus, gizzard, intestine, pancreas, heart, pectoral muscle, ovary, and testes were collected from infected birds for pathological and immunohistochemical examinations.

Results: Clinical signs were generally nonspecific and comprised weakness, dehydration, and emaciation. Gross lesions were mostly in the liver and spleen, in the form of minute white nodules scattered on the liver surface. Microscopic examination of the liver, spleen, and kidneys showed masses of uniform sizes and the presence of differentiated lymphoid cells. These cells appeared as large mononuclear cells with poorly defined cell membranes. Immunohistochemical investigation exhibited that the ALV-A positive indicators were chiefly accessible in the liver, ovary, spleen, and kidney.

Conclusion: Lymphoid leukosis in pigeons could be provisionally diagnosed by a pathological picture of characteristic tumors and confirmed by immunoreactivity of viral antigens in different tissues.

背景:鸽白血病主要由禽白血病病毒A亚群(ALV-A)引起。目的:本研究旨在对埃及自然感染鸽群的淋巴白血病进行诊断:方法:收集受感染鸽子的肝、脾、胸腺、肾、肺、胃窦、胗、肠、胰腺、心脏、胸肌、卵巢和睾丸组织标本,进行病理和免疫组化检查:临床症状一般无特异性,包括虚弱、脱水和消瘦。大体病变主要发生在肝脏和脾脏,表现为散布在肝脏表面的微小白色结节。肝脏、脾脏和肾脏的显微镜检查显示出大小一致的肿块和分化的淋巴细胞。这些细胞呈大的单核细胞,细胞膜轮廓不清。免疫组化检查显示,ALV-A 阳性指标主要见于肝脏、卵巢、脾脏和肾脏:结论:鸽子的淋巴白血病可通过特征性肿瘤的病理图像进行初步诊断,并通过不同组织中病毒抗原的免疫反应进行确诊。
{"title":"Pathological and immunohistochemical studies of lymphoid leukosis in pigeons in Egypt.","authors":"Abdelmenem Ahmed Elmeligy, Ali A Ghania, Ahmed Fotouh","doi":"10.5455/OVJ.2024.v14.i8.24","DOIUrl":"10.5455/OVJ.2024.v14.i8.24","url":null,"abstract":"<p><strong>Background: </strong>Pigeon leukosis is primarily caused by avian leukosis virus subgroup A (ALV-A). It infects and transforms lymphoid cells, leading to the development of tumors in various lymphoid tissues and other organs especially the liver.</p><p><strong>Aim: </strong>This study was conducted to diagnose lymphoid leukosis in a naturally infected pigeon flock in Egypt.</p><p><strong>Methods: </strong>Tissue specimens from the liver, spleen, thymus, kidney, lung, proventriculus, gizzard, intestine, pancreas, heart, pectoral muscle, ovary, and testes were collected from infected birds for pathological and immunohistochemical examinations.</p><p><strong>Results: </strong>Clinical signs were generally nonspecific and comprised weakness, dehydration, and emaciation. Gross lesions were mostly in the liver and spleen, in the form of minute white nodules scattered on the liver surface. Microscopic examination of the liver, spleen, and kidneys showed masses of uniform sizes and the presence of differentiated lymphoid cells. These cells appeared as large mononuclear cells with poorly defined cell membranes. Immunohistochemical investigation exhibited that the ALV-A positive indicators were chiefly accessible in the liver, ovary, spleen, and kidney.</p><p><strong>Conclusion: </strong>Lymphoid leukosis in pigeons could be provisionally diagnosed by a pathological picture of characteristic tumors and confirmed by immunoreactivity of viral antigens in different tissues.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vivo assessment of the anti-benign prostatic hyperplasia effect of hot ethanolic extract of flax seeds in male rats. 亚麻籽乙醇热提取物对雄性大鼠抗良性前列腺增生作用的体内评估
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.21
Jamela K Abd-Alhussen, Masar J Al-Kurdy, Suha A Hussein

Background: The most widespread condition that affected on primarily the male population is Benign hyperplasia of the prostate benign prostatic hyper-plasia (BPH). Flax seeds have been reported to have antiproliferation properties and exhibit antitumor.

Aim: We assessed the impact of flax seeds ethanolic excerpt on BPH within a testosterone propionate (TP)-induced model of rats.

Methods: A pre-3-week daily injection of TP (3 mg/kg BW) was used to induce BPH. Twenty male rats (200-240 gm) were randomly divided into 4 equal groups (n = 5) negative Group under control was given PBS orally, corn oil S/C, BPH-induced rats received 3 mg/kg BW TP for 3 weeks, extract group received 50 mg/Kg extract twice daily for 2 weeks Finasteride group received standard drug 10 mg/Kg BW for 2 weeks. When the course of treatment is over, rats were sacrificed and the blood was collected and separated, the prostate of the rats was harvested for histological examination.

Results: The results showed that flax seeds ethanolic extract could significantly (p < 0.05) reduce the prostate gland weight, prostate index, serum level of PAS, testosterone, and 5-a reductase enzyme in BPH-induced rats and improve the tissue morphology of the prostate.

Conclusion: Based on our results, the extract suggested that have a promising role in the treatment of benign hyperplasia of the prostate.

背景:前列腺良性增生症(BPH)是主要影响男性人群的最常见疾病。目的:在丙酸睾酮(TP)诱导的大鼠模型中,我们评估了亚麻籽乙醇提取物对良性前列腺增生症的影响:方法:在诱导良性前列腺增生症的模型中,大鼠在3周前每天注射3毫克/千克体重的丙酸睾酮(TP)。将 20 只雄性大鼠(200-240 克)随机分为 4 组(n = 5),阴性对照组口服 PBS,玉米油 S/C,良性前列腺增生诱导大鼠接受 3 毫克/千克体重的 TP,连续 3 周;提取物组接受 50 毫克/千克提取物,每天两次,连续 2 周;非那雄胺组接受标准药物 10 毫克/千克体重,连续 2 周。疗程结束后,大鼠被处死,收集并分离血液,采集大鼠的前列腺进行组织学检查:结果:亚麻籽乙醇提取物能显著(P < 0.05)降低良性前列腺增生大鼠的前列腺重量、前列腺指数、血清 PAS、睾酮和 5-a 还原酶水平,并改善前列腺的组织形态:根据我们的研究结果,该提取物有望在治疗前列腺良性增生症中发挥作用。
{"title":"<i>In vivo</i> assessment of the anti-benign prostatic hyperplasia effect of hot ethanolic extract of <i>flax</i> seeds in male rats.","authors":"Jamela K Abd-Alhussen, Masar J Al-Kurdy, Suha A Hussein","doi":"10.5455/OVJ.2024.v14.i8.21","DOIUrl":"10.5455/OVJ.2024.v14.i8.21","url":null,"abstract":"<p><strong>Background: </strong>The most widespread condition that affected on primarily the male population is Benign hyperplasia of the prostate benign prostatic hyper-plasia (BPH). Flax seeds have been reported to have antiproliferation properties and exhibit antitumor.</p><p><strong>Aim: </strong>We assessed the impact of flax seeds ethanolic excerpt on BPH within a testosterone propionate (TP)-induced model of rats.</p><p><strong>Methods: </strong>A pre-3-week daily injection of TP (3 mg/kg BW) was used to induce BPH. Twenty male rats (200-240 gm) were randomly divided into 4 equal groups (<i>n</i> = 5) negative Group under control was given PBS orally, corn oil S/C, BPH-induced rats received 3 mg/kg BW TP for 3 weeks, extract group received 50 mg/Kg extract twice daily for 2 weeks Finasteride group received standard drug 10 mg/Kg BW for 2 weeks. When the course of treatment is over, rats were sacrificed and the blood was collected and separated, the prostate of the rats was harvested for histological examination.</p><p><strong>Results: </strong>The results showed that flax seeds ethanolic extract could significantly (<i>p</i> < 0.05) reduce the prostate gland weight, prostate index, serum level of PAS, testosterone, and 5-a reductase enzyme in BPH-induced rats and improve the tissue morphology of the prostate.</p><p><strong>Conclusion: </strong>Based on our results, the extract suggested that have a promising role in the treatment of benign hyperplasia of the prostate.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Open Veterinary Journal
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1