Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya.

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.12
Fawzia Mohamed, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, Nagi Saeed
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Abstract

Background: The most common natural prion disease that primarily affects sheep and goats is scrapie. It belongs to a group of disorders known as transmissible spongiform encephalopathies, which impact both humans and animals.

Aim: The research is aimed to examine and confirm the presence of scrapie in Libya using immunohistochemistry (IHC) techniques.

Methods: Brain samples were collected from thirty-three sheep older than two years of age showing clinical signs resembling to scrapie during the period between 2018 and 2023, regardless of race or gender. Three animals, six months old, healthy, and without any symptoms, were used as negative controls. Different parts of the brain, including the obex and cerebellum, were taken from each case. The IHC technique used in this study involved staining with monoclonal antibody L42 and DAB (3,3'-diaminobenzidine) as a chromogenic substrate.

Results: The IHC examination showed the expression of prion proteins in brain tissue in twenty-three samples. The staining intensity was markedly observed in the neuronal cell bodies and around blood vessels.

Conclusion: The findings of this study provide evidence that scrapie exists in Libya.

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对利比亚东部自然疫羊瘙痒病的免疫组织化学研究。
背景:绵羊和山羊最常见的自然朊病毒病是痒病。目的:本研究旨在利用免疫组化(IHC)技术检查并确认利比亚是否存在绵羊瘙痒症:在 2018 年至 2023 年期间,从 33 只年龄超过两岁、出现类似痒病临床症状的绵羊身上采集脑样本,不分种族和性别。三只六个月大、健康、无任何症状的动物作为阴性对照。从每个病例中抽取大脑的不同部位,包括大脑外胚层和小脑。本研究采用的 IHC 技术包括用单克隆抗体 L42 和 DAB(3,3'-二氨基联苯胺)作为显色底物进行染色:结果:IHC 检查显示 23 个样本的脑组织中存在朊病毒蛋白。结果:IHC 检查显示,23 个样本的脑组织中均有朊病毒蛋白的表达,染色强度在神经元细胞体和血管周围明显可见:本研究结果为利比亚存在痒病提供了证据。
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来源期刊
Open Veterinary Journal
Open Veterinary Journal VETERINARY SCIENCES-
CiteScore
1.40
自引率
0.00%
发文量
112
审稿时长
12 weeks
期刊介绍: Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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