Relative biological effectiveness of clinically relevant photon energies for the survival of human colorectal, cervical, and prostate cancer cell lines.

IF 3.3 3区 医学 Q2 ENGINEERING, BIOMEDICAL Physics in medicine and biology Pub Date : 2024-10-04 DOI:10.1088/1361-6560/ad7d5a
Joanna Li, Naim Chabaytah, Joud Babik, Behnaz Behmand, Hamed Bekerat, Tanner Connell, Michael Evans, Russell Ruo, Te Vuong, Shirin Abbasinejad Enger
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Abstract

Objective.Relative biological effectiveness (RBE) differs between radiation qualities. However, an RBE of 1.0 has been established for photons regardless of the wide range of photon energies used clinically, the lack of reproducibility in radiobiological studies, and outdated reference energies used in the experimental literature. Moreover, due to intrinsic radiosensitivity, different cancer types have different responses to radiation. This study aimed to characterize the RBE of clinically relevant high and low photon energiesin vitrofor three human cancer cell lines: HCT116 (colon), HeLa (cervix), and PC3 (prostate).Approach.Experiments were conducted following dosimetry protocols provided by the American Association of Physicists in Medicine. Cells were irradiated with 6 MV x-rays, an192Ir brachytherapy source, 225 kVp and 50 kVp x-rays. Cell survival post-irradiation was assessed using the clonogenic assay. Survival fractions were fitted using the linear quadratic model, and survival curves were generated for RBE calculations.Main results.Cell killing was more efficient with decreasing photon energy. Using 225 kVp x-rays as the reference, the HCT116 RBESF0.1for 6 MV x-rays,192Ir, and 50 kVp x-rays were 0.89 ± 0.03, 0.95 ± 0.03, and 1.24 ± 0.04; the HeLa RBESF0.1were 0.95 ± 0.04, 0.97 ± 0.05, and 1.09 ± 0.03, and the PC3 RBESF0.1were 0.84 ± 0.01, 0.84 ± 0.01, and 1.13 ± 0.02, respectively. HeLa and PC3 cells had varying radiosensitivity when irradiated with 225 and 50 kVp x-rays.Significance.This difference supports the notion that RBE may not be 1.0 for all photons through experimental investigations that employed precise dosimetry. It highlights that different cancer types may not have identical responses to the same irradiation quality. Additionally, the RBE of clinically relevant photons was updated to the reference energy of 225 kVp x-rays.

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临床相关光子能量对人类结直肠癌、宫颈癌和前列腺癌细胞系存活的相对生物有效性。
目标:不同辐射质量的相对生物效应(RBE)是不同的。然而,尽管临床上使用的光子能量范围很广,放射生物学研究缺乏可重复性,而且实验文献中使用的参考能量已经过时,但光子的 RBE 仍被确定为 1.0。此外,由于固有的辐射敏感性,不同类型的癌症对辐射的反应也不尽相同。本研究旨在描述三种人类癌症细胞系在体外接受临床相关的高光子能量和低光子能量时的 RBE 特性:方法:实验按照美国医学物理学家协会提供的剂量测定方案进行。细胞接受 6 MV X 射线、192Ir 近距离放射源、225 kVp 和 50 kVp X 射线照射。细胞辐照后的存活率通过克隆生成试验进行评估。主要结果:光子能量越低,细胞杀伤效率越高:HCT116 RBESF0.1分别为 0.89 ± 0.03、0.95 ± 0.03 和 1.24 ± 0.04;HeLa RBESF0.1分别为 0.95 ± 0.04、0.97 ± 0.05 和 1.09 ± 0.03,PC3 RBESF0.1 分别为 0.84 ± 0.01、0.84 ± 0.01 和 1.13 ± 0.02。HeLa和PC3细胞在接受225 kVp和50 kVp X射线照射时具有不同的放射敏感性。它强调了不同类型的癌症对相同辐照质量的反应可能不尽相同。此外,临床相关光子的 RBE 已更新为 225 kVp X 射线的参考能量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physics in medicine and biology
Physics in medicine and biology 医学-工程:生物医学
CiteScore
6.50
自引率
14.30%
发文量
409
审稿时长
2 months
期刊介绍: The development and application of theoretical, computational and experimental physics to medicine, physiology and biology. Topics covered are: therapy physics (including ionizing and non-ionizing radiation); biomedical imaging (e.g. x-ray, magnetic resonance, ultrasound, optical and nuclear imaging); image-guided interventions; image reconstruction and analysis (including kinetic modelling); artificial intelligence in biomedical physics and analysis; nanoparticles in imaging and therapy; radiobiology; radiation protection and patient dose monitoring; radiation dosimetry
期刊最新文献
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