Pathological and vascular changes in the rat testiсle after experimental trauma.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-09-20 DOI:10.5653/cerm.2024.07080
Anastasiya Spaska, Bogdan Grytsuliak, Nelia Dolynko
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Abstract

Objective: Mechanical trauma to the testicles poses a potential risk of tissue destruction, disruption of local blood supply, and impairment of spermatogenesis, which can ultimately lead to infertility. Therefore, investigating this topic is crucial. The study aimed to identify cytological and morphological changes in the testicular tissue of laboratory rats following mechanical trauma to the organ.

Methods: Observations were recorded on days 7, 14, 30, and 90 post-trauma. The experiment involved two groups of animals: a control group of healthy animals and an experimental group that sustained blunt mechanical trauma. Tissue samples were collected, fixed, dehydrated, and embedded in paraffin; subsequently, sections were prepared and stained. Structural changes in tissues and cells were documented using light and transmission electron microscopy.

Results: In the experimental sample, notable changes included a decrease in organ weight, thickening of the protein shell and tubule walls, sclerotisation of the tubule membrane, narrowing of tubule diameter, reduced spermatozoa and spermatids titre, diminished capillary network and spermatogenic epithelium, uneven blood vessel lumen expansion, and decreased volume of Leydig cell nuclei. Additionally, in cells under different functional loads, the cytoplasm was vacuolated, mitochondrial cristae and the Golgi apparatus were diminished, cytoplasm volume decreased, karyopyknosis was observed, and uncharacteristic protrusions appeared on the surface of the cytoplasmic membrane. The severity of destruction at the cellular and tissue levels showed a positive correlation with time.

Conclusion: The data obtained from these model sites can be predictive for clinical trials.

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实验性创伤后大鼠睾丸的病理和血管变化
目的:睾丸受到机械性创伤可能会造成组织破坏、局部供血中断和精子生成障碍,最终导致不育。因此,研究这一课题至关重要。本研究旨在确定实验鼠睾丸组织在受到机械性创伤后的细胞学和形态学变化:方法:在创伤后第 7、14、30 和 90 天进行观察记录。实验包括两组动物:健康动物对照组和遭受钝性机械创伤的实验组。采集的组织样本经固定、脱水和石蜡包埋后,制备切片并染色。使用光镜和透射电子显微镜记录组织和细胞的结构变化:结果:在实验样本中,明显的变化包括器官重量减轻、蛋白外壳和小管壁增厚、小管膜硬化、小管直径变窄、精子和精子滴度降低、毛细血管网和生精上皮减少、血管腔扩张不均匀以及莱迪格细胞核体积缩小。此外,在不同功能负荷下的细胞中,细胞质呈空泡化,线粒体嵴和高尔基体减少,细胞质体积缩小,出现核分裂,细胞质膜表面出现不典型的突起。细胞和组织层面的破坏严重程度与时间呈正相关:结论:从这些模型部位获得的数据对临床试验具有预测作用。
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