Combination effect of levofloxacin and cefmenoxime against ocular isolates of Pseudomonas aeruginosa.

IF 4.1 3区医学 Q1 OPHTHALMOLOGY Contact Lens & Anterior Eye Pub Date : 2024-09-20 DOI:10.1016/j.clae.2024.102311

Motohiro Itoi, Mark D P Willcox

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Abstract

Purpose: This study aimed to investigate the effects of the combination of levofloxacin (LVFX) and cefmenoxime (CMX) on ocular isolates of Pseudomonas aeruginosa.

Methods: Twenty-three strains of P. aeruginosa isolated from microbial keratitis cases in Australia were utilized in this study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each antibiotic alone were determined and the combined susceptibility of antibiotics was assessed through fractional inhibitory concentration index (FICI). Additionally, tolerance of a ciprofloxacin tolerant P. aeruginosa isolate to each antibiotic alone and combination were analyzed.

Results: The median MIC and MBC values of CMX against P. aeruginosa were 16 µg/mL and 32 µg/mL, respectively. The median MIC and MBC values of LVFX were 0.5 µg/mL and 1 µg/mL, respectively, and all isolates were susceptible. In dual combination, the median of FICI of all strains was 0.63; seven strains showed synergistic effects (FICI≤0.5), and fifteen strains showed additive activities, while no strains exhibited antagonism. The isolate of P. aeruginosa that had previously exhibited tolerance to ciprofloxacin showed high tolerance to CMX alone and low tolerance to LVFX alone. This strain showed low tolerance to the combination of CMX and LVFX.

Conclusion: The combination of LVFX with CMX may have beneficial interactions which increase antibacterial activity against P. aeruginosa isolates and may be a feasible option for addressing the issue of bacteria resistant to each antibiotic.

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左氧氟沙星和头孢甲肟联用对眼部铜绿假单胞菌分离株的疗效。

目的：本研究旨在探讨左氧氟沙星（LVFX）和头孢甲肟（CMX）联合用药对眼部铜绿假单胞菌分离株的影响：本研究采用了从澳大利亚微生物角膜炎病例中分离出的 23 株铜绿假单胞菌。测定了每种抗生素单独使用时的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），并通过分数抑菌浓度指数（FICI）评估了抗生素的综合敏感性。此外，还分析了耐环丙沙星的铜绿微囊桿菌分离株对每种抗生素单独使用和联合使用的耐受性：结果：CMX 对铜绿假单胞菌的中位 MIC 值和 MBC 值分别为 16 µg/mL 和 32 µg/mL。LVFX 的中位 MIC 值和 MBC 值分别为 0.5 µg/mL 和 1 µg/mL，所有分离菌株均对其敏感。在双重组合中，所有菌株的 FICI 中值为 0.63；7 种菌株表现出协同效应（FICI≤0.5），15 种菌株表现出相加活性，没有菌株表现出拮抗作用。之前对环丙沙星耐受的铜绿假单胞菌分离株对 CMX 单药耐受性较高，对 LVFX 单药耐受性较低。该菌株对 CMX 和 LVFX 的组合耐受性较低：结论：LVFX 与 CMX 的组合可能会产生有益的相互作用，从而提高对铜绿假单胞菌分离株的抗菌活性，这可能是解决细菌对每种抗生素产生耐药性问题的一个可行方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Contact Lens & Anterior Eye OPHTHALMOLOGY-

CiteScore

7.60

自引率

18.80%

发文量

198

审稿时长

55 days

期刊介绍： Contact Lens & Anterior Eye is a research-based journal covering all aspects of contact lens theory and practice, including original articles on invention and innovations, as well as the regular features of: Case Reports; Literary Reviews; Editorials; Instrumentation and Techniques and Dates of Professional Meetings.