Longitudinal two-year comparative genomic analysis of respiratory Staphylococcus aureus isolates from intensive care unit mechanically ventilated patients
S. Meyer , A.C. Hernandez-Padilla , A-L. Fedou , T. Daix , D. Chainier , M-C. Ploy , P. Vignon , B. François , O. Barraud
{"title":"Longitudinal two-year comparative genomic analysis of respiratory Staphylococcus aureus isolates from intensive care unit mechanically ventilated patients","authors":"S. Meyer , A.C. Hernandez-Padilla , A-L. Fedou , T. Daix , D. Chainier , M-C. Ploy , P. Vignon , B. François , O. Barraud","doi":"10.1016/j.jhin.2024.09.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Ventilator-associated pneumonia (VAP) is the main healthcare-associated infection in intensive care units with <em>Staphylococcus aureus</em> as the first pathogen in early VAP.</div></div><div><h3>Aim</h3><div>To compare, using whole-genome sequencing (WGS), consecutive <em>S. aureus</em> isolates from lower respiratory samples of mechanically ventilated patients for identification of potential cross-transmissions; and to determine a potential link between <em>S. aureus</em> WGS data and patients with <em>S. aureus</em> early VAP.</div></div><div><h3>Methods</h3><div>All mechanically ventilated patients with a documentation of respiratory <em>S. aureus</em> isolates were included over a two-year period. WGS allowed typing, comparative genomic and phylogenic analyses, as well as analyses of antibiotic resistance genes and virulence genes. Virulence genes were compared between patients who developed respiratory infectious event and those who did not.</div></div><div><h3>Findings</h3><div>A total of 172 <em>S. aureus</em> isolates from 167 patients were sequenced. WGS revealed that the <em>S. aureus</em> population was polyclonal with only two potential healthcare cross-transmissions, each involving two isolates (2.3%). A very low resistance rate was observed with a strong genotypic/phenotypic association, and with a virulence profile highly dependent on the sequence type. No significant correlation was observed between VAP and virulence profile.</div></div><div><h3>Conclusion</h3><div>This study on consecutive respiratory <em>S. aureus</em> isolates of mechanically ventilated patients revealed a very low level of cross-transmission. No association was observed between <em>S. aureus</em> WGS data and VAP occurrence.</div></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"154 ","pages":"Pages 37-44"},"PeriodicalIF":3.9000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hospital Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0195670124002986","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Ventilator-associated pneumonia (VAP) is the main healthcare-associated infection in intensive care units with Staphylococcus aureus as the first pathogen in early VAP.
Aim
To compare, using whole-genome sequencing (WGS), consecutive S. aureus isolates from lower respiratory samples of mechanically ventilated patients for identification of potential cross-transmissions; and to determine a potential link between S. aureus WGS data and patients with S. aureus early VAP.
Methods
All mechanically ventilated patients with a documentation of respiratory S. aureus isolates were included over a two-year period. WGS allowed typing, comparative genomic and phylogenic analyses, as well as analyses of antibiotic resistance genes and virulence genes. Virulence genes were compared between patients who developed respiratory infectious event and those who did not.
Findings
A total of 172 S. aureus isolates from 167 patients were sequenced. WGS revealed that the S. aureus population was polyclonal with only two potential healthcare cross-transmissions, each involving two isolates (2.3%). A very low resistance rate was observed with a strong genotypic/phenotypic association, and with a virulence profile highly dependent on the sequence type. No significant correlation was observed between VAP and virulence profile.
Conclusion
This study on consecutive respiratory S. aureus isolates of mechanically ventilated patients revealed a very low level of cross-transmission. No association was observed between S. aureus WGS data and VAP occurrence.
期刊介绍：
The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience.
The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that:
provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings;
provide new insight into cleaning, disinfection and decontamination;
provide new insight into the design of healthcare premises;
describe novel aspects of outbreaks of infection;
throw light on techniques for effective antimicrobial stewardship;
describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control;
improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change;
improve understanding of the use of IT systems in infection surveillance and prevention and control.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
