BACH to the ferroptosis.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of biochemistry Pub Date : 2024-09-24 DOI:10.1093/jb/mvae064
Fuminori Tokunaga
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引用次数: 0

Abstract

Ferroptosis is regulated cell death characterized by iron-dependent phospholipid peroxidation, and is closely related to various diseases. System Xc -, a cystine/glutamate antiporter, and glutathione peroxidase 4 (GPX4) are the key molecules in ferroptosis. Erastin and RSL3, known as inhibitors of system Xc - and GPX4, respectively, are commonly used as ferroptosis inducers. BTB and CNC homology 1 (BACH1), a heme-binding transcription repressor, promotes pro-ferroptotic signaling, and therefore, Bach1-deficient cells are resistant to ferroptosis. Irikura et al. constructed Bach1-re-expressing immortalized mouse embryonic fibroblasts (iMEFs) from Bach1-/- mice, which induce ferroptosis simply by the depletion of 2-mercaptoethanol from the culture medium (J. Biochem. 2023; 174:239-252). Transcriptional repression by re-expressed BACH1 induces suppressed glutathione synthesis and increases labile iron. Furthermore, the ferroptosis initiated by BACH1-re-expressing iMEFs is propagated to surrounding cells. Thus, the BACH1-re-expression system is a novel and powerful tool to investigate the cellular basis of ferroptosis.

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BACH to the ferroptosis.
铁变态反应是以铁依赖性磷脂过氧化为特征的调节性细胞死亡,与多种疾病密切相关。胱氨酸/谷氨酸反转运体 Xc 系统和谷胱甘肽过氧化物酶 4(GPX4)是铁跃变的关键分子。Erastin 和 RSL3 分别被称为 Xc 系统和 GPX4 的抑制剂,常用作铁细胞色素沉着诱导剂。BTB 和 CNC 同源体 1(BACH1)是一种血红素结合转录抑制因子,可促进促铁蛋白沉降的信号转导,因此 Bach1 缺失的细胞对铁蛋白沉降具有抗性。Irikura 等人从 Bach1-/- 小鼠中构建了重新表达 Bach1 的永生化小鼠胚胎成纤维细胞(iMEFs),只需从培养基中去除 2-巯基乙醇就能诱导铁氧化(《生物化学杂志》,2023 年;174:239-252)。重新表达的 BACH1 会抑制谷胱甘肽的合成,并增加可溶性铁。此外,重新表达 BACH1 的 iMEFs 启动的铁变态反应会传播到周围的细胞。因此,BACH1-再表达系统是研究铁变态反应细胞基础的一种新颖而强大的工具。
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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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