Overexpression of GmSRC2 confers resistance to Phytophthora sojae in soybean

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Science Pub Date : 2024-09-21 DOI:10.1016/j.plantsci.2024.112247

Sushuang Deng, Yu Zhang, Xiaowan Fang, Han Gou, Ruidong Sun, Huidong Xuan, Haitang Wang, Jinming Zhao, Han Xing, Na Guo

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Abstract

Phytophthora root and stem rot caused by Phytophthora sojae (P. sojae) is one of the most destructive diseases to affect soybean (Glycine max (L.) Merr) production. GmSRC2 that encodes a C2 domain-containing protein can respond to various stresses, however, the molecular mechanism of GmSRC2 in resistance of soybean to P. sojae is yet to be fully elucidated. In this study, GmSRC2 was found to be significantly up-regulated under P. sojae treatment; GmSRC2-overexpression (OE) transgenic lines and GmSRC2-silencing transient plants were generated via Agrobacterium tumefaciens mediated transformation and virus-induced gene silencing (VIGS) system, respectively. Infected leaves and cotyledons of OE-GmSRC2–1 and OE-GmSRC2–2 lines showed significant decreases in the disease symptoms and P. sojae biomass than those of wild type (WT); the activities of superoxide dismutase (SOD) and peroxidase (POD) confirmed the accumulation of reactive oxygen species (ROS) in overexpressed transgenic lines. Whereas, silencing of GmSRC2 severely increased the disease symptoms and the biomass of P. sojae. Further, we confirmed that GmSRC2 interacted with the effector PsAvh23 of P. sojae, and the C2 domain was crucial for the interaction. Overexpression of GmSRC2 upregulated the ADA2/GCN5 module upon P. sojae. The aforementioned results demonstrated that GmSRC2 played vital roles in regulating soybean resistance to oomycetes.

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过表达 GmSRC2 可增强大豆对根瘤蚜的抗性

大豆根腐病和茎腐病（Phytophthora root and stem rot，P. sojae）是影响大豆（Glycine max (L.) Merr）生产的最具破坏性的病害之一。编码含 C2 结构域蛋白的 GmSRC2 可对各种胁迫做出响应，但 GmSRC2 在大豆抗病中的分子机制尚未完全阐明。本研究发现 GmSRC2 在 P. sojae 处理下显著上调；通过农杆菌介导的转化和病毒诱导的基因沉默（VIGS）系统分别产生了 GmSRC2 外表达（OE）转基因株系和 GmSRC2 沉默瞬态植株。与野生型（WT）相比，OE-GmSRC2-1 和 OE-GmSRC2-2 株系受感染的叶片和子叶的病害症状和 P. sojae 生物量明显减少；超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性证实了过表达转基因株系中活性氧（ROS）的积累。而 GmSRC2 的沉默则严重增加了病害症状和 P. sojae 的生物量。此外，我们还证实了 GmSRC2 与 P. sojae 的效应物 PsAvh23 的相互作用，而 C2 结构域是相互作用的关键。过表达 GmSRC2 会上调 P. sojae 的 ADA2/GCN5 模块。上述结果表明，GmSRC2 在调控大豆对卵菌的抗性中发挥了重要作用。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.