Perturbation of enzyme structure by nano-metal organic frameworks: A question mark on their safety-by-design?

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL Journal of hazardous materials letters Pub Date : 2024-09-20 DOI:10.1016/j.hazl.2024.100127
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Abstract

Our study investigates the interactions between nanoscale Metal-Organic Frameworks (nMOFs), specifically ZIF-8 and CuIm, and key enzymes: Acetylcholine Esterase (AChE), α-amylase. Using circular dichroism (CD) spectroscopy, we observed significant alterations in the secondary structures of these enzymes upon interaction with nMOFs. AChE showed a reduction in α-helix content from 20.1 % to a significantly lower value when exposed to 160 µg/mL of nMOFs, with a corresponding increase in β-sheet and other structural components. Enzymatic activity assays revealed that CuIm nMOFs decreased AChE activity by 67.08 % at the highest concentration tested (160 µg/mL). ZIF-8 also affected AChE activity significantly at this concentration. Similarly, α-amylase exhibited structural changes, with increasing concentrations of nMOFs leading to a near-total loss of secondary structure at 80 and 160 µg/mL. These structural changes were accompanied by a marked decrease in enzymatic activity, particularly with CuIm nMOFs showing the most substantial inhibitory effects. Our findings highlight the profound impact of nMOFs on enzyme structures and functions, emphasising the need for comprehensive assessments of nMOFs' potential toxicity and understanding the aspects of their safety-by-design.
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纳米金属有机框架对酶结构的干扰:设计安全性的问号?
我们的研究调查了纳米级金属有机框架(nMOFs),特别是 ZIF-8 和 CuIm,与关键酶之间的相互作用:乙酰胆碱酯酶(AChE)、α-淀粉酶。利用圆二色性(CD)光谱,我们观察到这些酶与 nMOFs 相互作用时二级结构发生了显著变化。当暴露于 160 µg/mL 的 nMOFs 时,AChE 的 α-螺旋含量从 20.1% 降至明显较低的值,而 β-片和其他结构成分则相应增加。酶活性测定显示,在测试的最高浓度(160 微克/毫升)下,CuIm nMOFs 可使 AChE 活性降低 67.08%。在此浓度下,ZIF-8 也会显著影响 AChE 的活性。同样,α-淀粉酶的结构也发生了变化,随着 nMOFs 浓度的增加,在 80 µg/mL 和 160 µg/mL 浓度下,二级结构几乎完全丧失。伴随这些结构变化的是酶活性的明显降低,尤其是 CuIm nMOF 的抑制作用最为显著。我们的研究结果突显了 nMOFs 对酶结构和功能的深远影响,强调了全面评估 nMOFs 潜在毒性和了解其设计安全性的必要性。
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来源期刊
Journal of hazardous materials letters
Journal of hazardous materials letters Pollution, Health, Toxicology and Mutagenesis, Environmental Chemistry, Waste Management and Disposal, Environmental Engineering
CiteScore
10.30
自引率
0.00%
发文量
0
审稿时长
20 days
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