Peptide-based fluorescent probe with large Stokes shift for specific sequential detection of copper (II) ions and histidine: Smartphone device, real samples, test strips and bioimaging applications
{"title":"Peptide-based fluorescent probe with large Stokes shift for specific sequential detection of copper (II) ions and histidine: Smartphone device, real samples, test strips and bioimaging applications","authors":"","doi":"10.1016/j.microc.2024.111728","DOIUrl":null,"url":null,"abstract":"<div><div>A novel and facile fluorescent probe, <strong>DNH</strong>, was developed based on a dipeptide backbone (Asn-His-NH<sub>2</sub>) labelled with a dansyl fluorophore. <strong>DNH</strong> exhibited significant advantages in detecting Cu<sup>2+</sup> based on the paramagnetic quenching mechanism, including satisfactory water solubility (100 % aqueous medium), a large Stokes shift (230 nm), excellent anti-interference capability (no interference), a low detection limit (19.6 nM), rapid response time (within 30 s) and a wide pH range (7–12). Employing a displacement approach, the <strong>DNH</strong>-Cu<sup>2+</sup> ensemble showed strong fluorescence enhancement upon recognition of <sub>L</sub>-His with outstanding selectivity, and the detection limit for the <strong>DNH</strong>-Cu<sup>2+</sup> ensemble with respect to <sub>L</sub>-His was calculated at 22.8 nM. Notably, <strong>DNH</strong> demonstrated favourable reversibility for at least seven cycles with minor changes in fluorescence intensity when Cu<sup>2+</sup> and <sub>L</sub>-His were added alternately. Moreover, <strong>DNH</strong> exhibited excellent low cytotoxicity and good biocompatibility, and was utilised as an outstanding probe for the sequential determination of Cu<sup>2+</sup> and <sub>L</sub>-His in living cells and zebrafish. <strong>DNH</strong> was successfully used to determine Cu<sup>2+</sup> and <sub>L</sub>-His in three actual water samples with good recovery and accuracy. In addition, <strong>DNH</strong> was applied to filter paper to develop visual test strips for the rapid and high-efficiency analysis of Cu<sup>2+</sup> and <sub>L</sub>-His. Furthermore, the semi-quantitative visual monitoring of Cu<sup>2+</sup> and <sub>L</sub>-His was achieved by developing a smartphone colour recogniser into a portable analytical device, achieving low detection limits of 0.49 μM and 0.63 μM, respectively. Given these attributes, <strong>DNH</strong> presents itself as a promising peptide-based fluorescent probe, showcasing considerable potential for real-time visual monitoring of Cu<sup>2+</sup> and <sub>L</sub>-His in various environmental and biological settings.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0026265X2401840X","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A novel and facile fluorescent probe, DNH, was developed based on a dipeptide backbone (Asn-His-NH2) labelled with a dansyl fluorophore. DNH exhibited significant advantages in detecting Cu2+ based on the paramagnetic quenching mechanism, including satisfactory water solubility (100 % aqueous medium), a large Stokes shift (230 nm), excellent anti-interference capability (no interference), a low detection limit (19.6 nM), rapid response time (within 30 s) and a wide pH range (7–12). Employing a displacement approach, the DNH-Cu2+ ensemble showed strong fluorescence enhancement upon recognition of L-His with outstanding selectivity, and the detection limit for the DNH-Cu2+ ensemble with respect to L-His was calculated at 22.8 nM. Notably, DNH demonstrated favourable reversibility for at least seven cycles with minor changes in fluorescence intensity when Cu2+ and L-His were added alternately. Moreover, DNH exhibited excellent low cytotoxicity and good biocompatibility, and was utilised as an outstanding probe for the sequential determination of Cu2+ and L-His in living cells and zebrafish. DNH was successfully used to determine Cu2+ and L-His in three actual water samples with good recovery and accuracy. In addition, DNH was applied to filter paper to develop visual test strips for the rapid and high-efficiency analysis of Cu2+ and L-His. Furthermore, the semi-quantitative visual monitoring of Cu2+ and L-His was achieved by developing a smartphone colour recogniser into a portable analytical device, achieving low detection limits of 0.49 μM and 0.63 μM, respectively. Given these attributes, DNH presents itself as a promising peptide-based fluorescent probe, showcasing considerable potential for real-time visual monitoring of Cu2+ and L-His in various environmental and biological settings.
期刊介绍:
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.