Recent advances in biosensors for analysis of DNA/RNA methylation

IF 5.4 Q1 CHEMISTRY, ANALYTICAL Sensing and Bio-Sensing Research Pub Date : 2024-09-17 DOI:10.1016/j.sbsr.2024.100690

Zhensheng Ma , Yue Hu , Lina Wang , Mimi Li , Chenghong Li , Lulu Li , Hui Huang , Lichao Fang , Xiaolong Wang , Huamin Liu , Junsong Zheng

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Abstract

DNA and RNA methylation are essential epigenetic modifications that play a pivotal role in various physiological processes by modulating gene expression at the post-transcriptional level. Abnormal DNA/RNA methylation is closely linked to a range of human diseases, including cancer and neurological disorders. Therefore, the accurate and sensitive detection of DNA/RNA methylation is of great importance in both epigenetic biology research and disease diagnosis. Technological advancements have led to the comprehensive development of methylation detection methods. Among these, electrochemical techniques have garnered significant attention owing to their simplicity and effectiveness in detection. This review provides an overview of methylation detection technologies based on electrochemical principles developed over the past five years, including electrochemical, photoelectrochemical, electrochemiluminescence, and fluorescence methodologies. Additionally, it discusses the advantages and disadvantages of these approaches, challenges and advancements in methylation detection.

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用于分析 DNA/RNA 甲基化的生物传感器的最新进展

DNA 和 RNA 甲基化是重要的表观遗传修饰，通过在转录后水平调节基因表达，在各种生理过程中发挥着关键作用。DNA/RNA 甲基化异常与一系列人类疾病（包括癌症和神经系统疾病）密切相关。因此，准确灵敏地检测 DNA/RNA 甲基化对表观遗传生物学研究和疾病诊断都非常重要。技术的进步促进了甲基化检测方法的全面发展。其中，电化学技术因其检测简便、有效而备受关注。本综述概述了过去五年中基于电化学原理开发的甲基化检测技术，包括电化学、光电化学、电化学发光和荧光方法。此外，它还讨论了这些方法的优缺点、甲基化检测面临的挑战和取得的进展。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.