Sensing and Bio-Sensing Research最新文献

“All-on-a-Tube” POCT of Salmonella in large-volume sample 大容量样本中沙门氏菌的 "全试管 "POCT检测

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-10 DOI: 10.1016/j.sbsr.2024.100712

Lei Wang, Nana Jin, Meixuan Li, Jianhan Lin

Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive Salmonella detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 10¹ to 1.3 × 10⁴ CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.

低浓度致病菌的床旁检测（POCT）对于细菌污染的早期预警至关重要。本研究重建了一个一次性离心管，用于大容量样本中沙门氏菌的灵敏检测，从分离到检测的整个细菌检测过程都在离心管内进行。该离心管底部装有一个柔性搅拌器，内含一对圆形磁铁，通过直流电机快速旋转产生涡流，从而同时混合和捕获目标细菌。此外，还合成了免疫二氧化锰纳米花，用于标记目标细菌，然后模拟催化无色 TMB 底物变成蓝色 TMBox 产物。智能手机应用程序捕捉并分析产物图像，从而定量确定目标细菌。这种 POCT 离心管能有效地从 10 mL 样品中分离出约 80% 的目标细菌，并能在 1 小时内检测出 1.3 × 101 至 1.3 × 104 CFU/mL 范围内的目标细菌，检测限低至 13 CFU/mL。更重要的是，这种重构离心管具有成本低、操作简便和集成度高的特点，证明了其作为实验室消耗品在常规筛查中进行细菌检测的潜力。

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引用次数: 0

Design of flexible polyimide-based serpentine EMG sensor for AI-enabled fatigue detection in construction 设计基于柔性聚酰亚胺的蛇形肌电图传感器，用于人工智能建筑疲劳检测

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-08 DOI: 10.1016/j.sbsr.2024.100713

Yogesh Gautam, Houtan Jebelli

Physical fatigue and musculoskeletal disorders are critical health issues for construction workers, stemming from repetitive motions, heavy lifting, and awkward postures. These factors compromise worker well-being, productivity, and safety while increasing the risk of accidents and long-term health problems. Recent advancements in wearable health monitoring technology offer potential solutions, but current sensors encounter significant challenges in the dynamic construction environment. These include inadequate skin contact, increased contact impedance, and vulnerability to motion artifacts all of which degrade signal quality and reduce the accuracy of fatigue detection. This paper develops a fractal-based, flexible sensor for enhanced adaptability and accurate fatigue estimation. Finite element analysis compared five space-filling designs, with the serpentine curve exhibiting the highest contact area and lowest strain, making it the preferred choice for fabrication. Evaluations demonstrated significant improvements in signal-to-noise ratio (SNR) and motion artifact reduction, with the newly developed sensor achieving a 37 % to 59 % SNR improvement over commercial electrodes across different muscle groups. The developed flexible sensor was integrated with a fatigue-detecting framework based on a vision transformer model which provided an average accuracy of 87 % for fatigue detection. The developed sensor significantly enhances EMG signal quality and reliability, promising improved health monitoring and safety for construction workers.

身体疲劳和肌肉骨骼疾病是建筑工人面临的重要健康问题，它们源于重复性动作、重物搬运和笨拙的姿势。这些因素损害了工人的健康、生产率和安全，同时增加了事故和长期健康问题的风险。可穿戴健康监测技术的最新进展提供了潜在的解决方案，但目前的传感器在动态的建筑环境中遇到了重大挑战。这些挑战包括皮肤接触不足、接触阻抗增加以及易受运动伪影影响，所有这些都会降低信号质量并降低疲劳检测的准确性。本文开发了一种基于分形的柔性传感器，以提高适应性和疲劳估算的准确性。有限元分析比较了五种空间填充设计，其中蛇形曲线的接触面积最大，应变最小，因此成为制造的首选。评估结果表明，新开发的传感器在信噪比（SNR）和运动伪影减少方面均有显著改善，在不同肌肉群中的信噪比比商用电极提高了 37% 至 59%。开发的柔性传感器与基于视觉变压器模型的疲劳检测框架集成，疲劳检测的平均准确率达到 87%。所开发的传感器大大提高了肌电信号的质量和可靠性，有望改善建筑工人的健康监测和安全状况。

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引用次数: 0

Molecular displacement approach for the electrochemical detection of protein-bound propofol 电化学检测蛋白结合型异丙酚的分子置换方法

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-07 DOI: 10.1016/j.sbsr.2024.100710

David C. Ferrier, Janice Kiely, Richard Luxton

Propofol is one of the principal drugs used for the sedation of patients undergoing mechanical ventilation in intensive care units. The correct dosage of such sedative drugs is highly important, but current methods of determining infusion rates are limited and there is a lack of suitable methods for directly determining patient blood propofol concentrations. A significant challenge for the development of propofol sensors is that propofol demonstrates very high protein binding, leading to a low free fraction in blood. Here we present a method for improving the efficacy of an electrochemical propofol sensor by increasing the free fraction via a molecular displacement approach. When used in conjunction with a carbon nanotube/graphene oxide/iron oxide nanoparticle functionalised screen-printed electrode, it was found that this approach dramatically improved the sensor's sensitivity towards propofol. Ibuprofen was found to be the most effective displacement agent, with an optimal concentration of 30 mM. The resultant sensitivity was 2.82 nA/μg/ml/mm² with a coefficient of variation of 0.07, and the limit of detection was 0.2 μg/ml. This approach demonstrates high specificity towards drugs commonly administered to intensive care patients.

异丙酚是用于重症监护病房机械通气患者镇静的主要药物之一。这种镇静药物的正确剂量非常重要，但目前确定输注率的方法有限，而且缺乏直接确定患者血液中丙泊酚浓度的合适方法。开发异丙酚传感器面临的一个重大挑战是异丙酚与蛋白质的结合率非常高，导致其在血液中的游离部分较低。在此，我们介绍一种通过分子置换方法增加游离部分，从而提高电化学异丙酚传感器功效的方法。在与碳纳米管/氧化石墨烯/氧化铁纳米颗粒功能化丝网印刷电极结合使用时，我们发现这种方法大大提高了传感器对丙泊酚的灵敏度。发现布洛芬是最有效的置换剂，最佳浓度为 30 mM。结果灵敏度为 2.82 nA/μg/ml/mm2，变异系数为 0.07，检测限为 0.2 μg/ml。这种方法对重症监护患者常用药物具有很高的特异性。

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引用次数: 0

Biosensor for integrin inhibition of mammalian cell adhesion and migration using micropatterned cell culture substrate and retroreflective optical signaling 利用微图案细胞培养基底和逆反射光学信号抑制哺乳动物细胞粘附和迁移的整合素生物传感器

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-06 DOI: 10.1016/j.sbsr.2024.100714

Kyung Won Lee , Eun Kyeong Yang , Duc Long Nguyen , Soo A Park , Moon Suk Kim , Jae-Ho Kim , Min Young Lee , Hyun C. Yoon

Integrins are a family of transmembrane receptors that play a crucial role in cell adhesion and migration. Integrins can uniquely transduce biochemical signals bidirectionally across the membrane and physically link the cell-cell and cell-extracellular matrix (ECM) with ligand bonds. The arginyl-glycyl-aspartic acid (RGD) peptide motif is present in the ECM as a minimal recognition sequence for integrins. To leverage this property in cell-based therapy, RGD variants, such as cyclic-type RGDfK (c(RGDfK)), which share a similar structure with RGD but exhibit a higher affinity for integrins, have been developed. However, because most evaluation methods for newly developed RGD variants focus on affinity strength, tools for cellular effects are required. In this study, we developed a new platform that integrates micropatterned three-dimensional cell culture substrates with a non-spectroscopic optical analysis system to quantitatively analyze the effects of RGD variants on cell adhesion and migration. The specially micropatterned substrate provides a cell adhesive and migration area to provide a restricted analysis area. Owing to the characteristics of retroreflective Janus particles (RJPs), a non-spectroscopic optical analysis system provides long-term stable optical verification properties and a simple optical setup. These techniques were integrated to quantitatively determine the integrin inhibitory effect of various concentrations of RGD variant. To demonstrate the efficacy of the developed cellular level RGD variant testing platform, the model cell line L929 fibroblast and model RGD variant c(RGDfK) were analyzed ranging from 0 to 10 μM. The results showed that the developed system could effectively and quantitatively analyze the effects of RGD variants on cells across various concentrations.

整合素是一种跨膜受体，在细胞粘附和迁移中发挥着至关重要的作用。整合素能以独特的方式双向跨膜传递生化信号，并通过配体键将细胞-细胞和细胞-细胞外基质（ECM）物理连接起来。精氨酰-甘氨酰-天冬氨酸（RGD）肽基作为整合素的最小识别序列存在于 ECM 中。为了在细胞疗法中利用这一特性，人们开发了 RGD 变体，如环状 RGDfK（c(RGDfK)），它与 RGD 具有相似的结构，但对整合素具有更高的亲和力。然而，由于新开发的 RGD 变体的大多数评估方法都集中在亲和力强度上，因此需要细胞效应工具。在本研究中，我们开发了一种新平台，将微图案三维细胞培养基底与非光谱光学分析系统集成在一起，用于定量分析 RGD 变体对细胞粘附和迁移的影响。特殊的微图案基底提供了一个细胞粘附和迁移区域，从而提供了一个限定的分析区域。由于逆反射 Janus 粒子（RJPs）的特性，非光谱光学分析系统提供了长期稳定的光学验证特性和简单的光学设置。通过整合这些技术，可以定量确定不同浓度的 RGD 变体对整合素的抑制作用。为了证明所开发的细胞级 RGD 变体检测平台的有效性，研究人员对模型细胞系 L929 成纤维细胞和模型 RGD 变体 c(RGDfK) 进行了 0 至 10 μM 的分析。结果表明，所开发的系统能有效地定量分析不同浓度的 RGD 变体对细胞的影响。

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引用次数: 0

Advancements in electrochemiluminescence-based sensors for ultra-sensitive pesticide residue detection 基于电化学发光的超灵敏农药残留检测传感器的研究进展

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-04 DOI: 10.1016/j.sbsr.2024.100708

Irkham , Chico Permana Putra , Clianta Yudin Kharismasari , Salma Nur Zakiyyah , Isnaini Rahmawati , Ivandini Tribidasari Anggraningrum , Wulan Tri Wahyuni , Giovanni Valenti , Francesco Paolucci , Yeni Wahyuni Hartati

Pesticides are chemical substances utilized to prevent, control, and eradicate pests or organisms detrimental to crops, playing a pivotal role in minimizing agricultural losses and enhancing yields. However, improper pesticide use can lead to residue accumulation in the surrounding environment, including agricultural products, soil, and water. Detecting pesticide residues in the environment has become imperative. While various techniques such as chromatography, spectrophotometry, optical sensors, and electrochemical biosensors have been reported for pesticide residue detection, they face limitations such as large instrument size and low sensitivity. Currently, electrochemiluminescence (ECL) methods have been developed for pesticide detection. ECL methods can be combined with biosensor techniques or other molecule recognition element to enhance selectivity and specificity. ECL combines controllable electrochemical reaction properties with high photokinetic sensitivity. It can detect analytes with low detection limits (down to pM levels), ensuring rapid measurements and requiring minimal sample volumes for analysis. This literature review discusses the progress in research on detecting pesticide residues using ECL methods. The utilization of ECL, with its unique capabilities, signifies a promising avenue for addressing the shortcomings of traditional detection methods and advancing the field of pesticide residue analysis.

农药是用来预防、控制和消灭有害农作物的害虫或生物的化学物质，在减少农业损失和提高产量方面发挥着关键作用。然而，农药的不当使用会导致残留物在农产品、土壤和水等周围环境中积累。检测环境中的农药残留已成为当务之急。虽然色谱法、分光光度法、光学传感器和电化学生物传感器等多种技术已被报道用于检测农药残留，但它们都面临着仪器体积大、灵敏度低等局限性。目前，用于农药检测的电化学发光（ECL）方法已经开发出来。ECL 方法可与生物传感器技术或其他分子识别元件相结合，以提高选择性和特异性。ECL 结合了可控的电化学反应特性和高光动力学灵敏度。它可以检测到低检测限（低至 pM 级）的分析物，确保快速测量，分析时只需极少的样品量。本文献综述讨论了使用 ECL 方法检测农药残留的研究进展。ECL 具有独特的功能，它的使用为解决传统检测方法的不足和推动农药残留分析领域的发展开辟了一条前景广阔的道路。

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引用次数: 0

Determining factor of enzyme conjugates, bridge heterology and analytical variables of immunogens in prednisolone ELISA 泼尼松龙酶联免疫吸附试验中酶共轭物、桥异性和免疫原分析变量的决定因素

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-02 DOI: 10.1016/j.sbsr.2024.100700

Dinesh Kumar , Harinder Singh Oberoi , Subash Chandra Sonkar , Bidhan Chandra Koner , T.G. Shrivastav

In this study, adipic acid dihydrazide (ADH), carbohydrazide (CH), ethylenediamine (EDA), and urea (U) were used as spacer molecules, covalently linking prednisolone (PSL) to carrier proteins for immunogen preparation, and PSL to enzymes for enzyme conjugate preparation using N-hydroxysuccinimide (NHS)-mediated carbodiimide reactions. The resulting immunogens were used to generate antiserum in New Zealand white rabbits. Antibodies produced against immunogens with various spacers were tested for immunoreactivity with enzyme conjugates, both with and without spacers, in a total of twenty different combinations. All combinations demonstrated binding and were subsequently evaluated through displacement studies. Sensitivity and specificity tests revealed that the combination of PSL-21-HS-U-BSA-antibody with PSL-21-HS-HRP enzyme conjugate exhibited the best sensitivity (0.032 ng/mL) and limited cross-reactivity with other steroids. This combination was further examined for analytical parameters, showing recovery rates of 93.87–101.19 % for PSL from spiked human serum samples, with intra- and inter-assay CVs of <8.88 %. The serum PSL values obtained by this method showed strong correlation with a commercially available ELISA kit (r² = 0.97, n = 78).

本研究使用己二酸二酰肼（ADH）、碳酰肼（CH）、乙二胺（EDA）和尿素（U）作为间隔分子，将泼尼松龙（PSL）与载体蛋白共价连接以制备免疫原，并使用 N-羟基琥珀酰亚胺（NHS）介导的碳化二亚胺反应将 PSL 与酶共价连接以制备酶结合物。得到的免疫原用于在新西兰白兔身上产生抗血清。针对带有各种间隔物的免疫原产生的抗体与酶结合物进行了免疫反应性测试，包括带间隔物和不带间隔物的酶结合物，共有二十种不同的组合。所有组合都显示出了结合性，随后通过置换研究对其进行了评估。灵敏度和特异性测试表明，PSL-21-HS-U-BSA-抗体与 PSL-21-HS-HRP 酶结合物的组合灵敏度最高（0.032 ng/mL），与其他类固醇的交叉反应有限。对这一组合的分析参数进行了进一步检测，结果显示，从添加的人体血清样本中提取 PSL 的回收率为 93.87-101.19%，测定内和测定间的 CV 为 8.88%。该方法获得的血清 PSL 值与市售的酶联免疫吸附试剂盒显示出很强的相关性（r2 = 0.97，n = 78）。

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引用次数: 0

Towards yeast fermentation monitoring: Enhanced sensing performance with nanostructured platinum integrated microsensors array 酵母发酵监测：利用纳米结构铂集成微传感器阵列提高传感性能

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-01 DOI: 10.1016/j.sbsr.2024.100709

Nurul Izni Rusli , Ruben Van den Eeckhoudt , Catarina Fernandes , Filippo Franceschini , Dimitrios Konstantinidis , Kevin J. Verstrepen , Frederik Ceyssens , Michael Kraft , Irene Taurino

Effective and continuous monitoring of bioprocesses requires the parallel screening of multiple key parameters to enhance the processes and ultimately improve the quality of the end products. In this work, the development and characterization of only few square millimeters microfabricated multi-sensor array chip for analysis of yeast fermentation is described. We originally integrated platinum nanostructures (nano-Pt) on the microelectrodes by a simple, CMOS compatible, and scalable electrodeposition procedure. This step was proven to be pivotal to obtain highly sensitive and selective microsensors with minimal cross-talk and measurement variability. Nano-Pt enables reliable sensing at lower applied potentials, offering a promising solution to mitigate electrical cross-talk in closely integrated sensor configurations. The multi-sensor features potentiometric parallel-plate nanostructured electrodes for measuring pH, interdigitated nano-Pt electrodes for indirectly measuring microbial growth and activity by measuring the electrolyte conductivity, and microelectrodes based on nano-Pt for measuring dissolved oxygen (DO) and glucose via amperometry. Importantly, all-solid-state on-chip reference electrodes for potentiometric and amperometric sensors of this chip have been developed and characterized to enable standalone measurements and achieve true miniaturization, avoiding the need for external conventional reference electrodes. The chip includes a meander thin-film resistance temperature detector for temperature monitoring as well. Our platform represents the first step towards viable in-situ monitoring of lab-scale yeast fermentation and to control the homogeneity of process parameters in large scale bioreactors.

要对生物过程进行有效和持续的监控，就需要同时对多个关键参数进行筛选，以改进过程并最终提高最终产品的质量。在这项工作中，介绍了用于分析酵母发酵的仅几平方毫米微加工多传感器阵列芯片的开发和表征。我们最初通过一种简单、与 CMOS 兼容且可扩展的电沉积程序在微电极上集成了纳米铂结构（nano-Pt）。这一步骤被证明是获得高灵敏度和高选择性微传感器的关键，可将串扰和测量变异性降至最低。纳米铂能在较低的应用电位下实现可靠的传感，为减轻紧密集成的传感器配置中的电气串扰提供了一种可行的解决方案。这种多传感器具有用于测量 pH 值的电位计平行板纳米结构电极、通过测量电解质电导率间接测量微生物生长和活性的交错纳米铂电极，以及通过安培计测量溶解氧和葡萄糖的基于纳米铂的微电极。重要的是，为该芯片的电位和安培传感器开发并鉴定了全固态片上参比电极，以实现独立测量和真正的微型化，从而避免了外部传统参比电极的需要。该芯片还包括一个用于温度监测的蜿蜒薄膜电阻温度检测器。我们的平台迈出了可行的第一步，可对实验室规模的酵母发酵进行原位监测，并控制大规模生物反应器中工艺参数的均匀性。

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引用次数: 0

Biosynthesis of ZnO nanocomposites from Mentha spicata applications of antioxidant, antimicrobial and genotoxicity advances in MCF-7 cell line 薄荷纳米氧化锌复合材料的生物合成在 MCF-7 细胞系中的抗氧化、抗菌和遗传毒性应用进展

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-01 DOI: 10.1016/j.sbsr.2024.100707

Azhagu Madhavan Sivalingam

This study focuses on enhancing the, Spearmint (Mentha spicata), an aromatic herb indigenous to Europe and Asia, which is valued for its refreshing flavor and potential health benefits. This herb is widely utilized in culinary practices, medicinal applications, and cosmetic formulations. The ethanol extract derived from the M. spicata leaf contains is rich secondary metabolites with various bioactive properties: Specifically, such as tannins, flavonoids, and polyphenols are known for their antioxidant and anti-inflammatory effects, saponins help reduce cholesterol, and alkaloids have analgesic properties. The extract has a total flavonoid content (TFC) of 231.37 ± 2.05 mg GAE/g and a total phenol content (TPC) of 247.32 ± 5.07 mg GAE/g. Zinc oxide (ZnO) nanoparticles were synthesized from this extract and subsequently characterized through various analytical techniques. The absorbance measurement at 437 nm confirmed the successful nanoparticle synthesis. FTIR spectra showed water-related absorption bands at 3330 cm⁻¹ and 3337 cm⁻¹. Scanning electron microscopy (SEM) indicated the presence of spherical nanoparticles of about 25.4 nm, while transmission electron microscopy (TEM) illustrated particles ranging from 20 to 50 nm with high crystallinity and a lattice spacing of 0.297 nm. X-ray diffraction (XRD) analysis confirmed their crystalline structure with distinct Bragg reflections at 39.1°, 46.1°, 68.7°, and 79.2°, which corresponding to (111), (200), (220,311) planes, respectively (JCPDS card no. 01–080-1876). Energy-dispersive X-ray spectroscopy (EDX) confirmed the high purity of the synthesized ZnO nanocrystals. The nanoparticles demonstrated significant antioxidant activity with radical scavenging rates up to 97.33 %, effective antimicrobial properties, and notable anticancer activity, achieving 99.75 % inhibition of MCF-7 cells at 40 μg/mL. These findings highlight the nanoparticles' potential applications in health and medicine.

薄荷（Mentha spicata）是一种原产于欧洲和亚洲的芳香草本植物，因其清新的味道和潜在的健康益处而备受青睐。这种香草被广泛用于烹饪、医药和化妆品配方中。从刺五加叶中提取的乙醇萃取物含有丰富的次生代谢物，具有多种生物活性：其中，单宁酸、类黄酮和多酚具有抗氧化和消炎作用，皂甙有助于降低胆固醇，生物碱具有镇痛作用。提取物的总黄酮含量（TFC）为 231.37 ± 2.05 毫克 GAE/克，总酚含量（TPC）为 247.32 ± 5.07 毫克 GAE/克。用这种提取物合成了纳米氧化锌（ZnO）颗粒，随后通过各种分析技术对其进行了表征。在 437 纳米波长处测量的吸光度证实了纳米颗粒的成功合成。傅立叶变换红外光谱在 3330 cm-1 和 3337 cm-1 处显示了与水有关的吸收带。扫描电子显微镜（SEM）显示存在约 25.4 纳米的球形纳米颗粒，而透射电子显微镜（TEM）则显示颗粒大小为 20 至 50 纳米，结晶度高，晶格间距为 0.297 纳米。X 射线衍射（XRD）分析证实了它们的晶体结构，在 39.1°、46.1°、68.7° 和 79.2°处有明显的布拉格反射，分别对应于 (111)、(200) 和 (220,311) 平面（JCPDS 卡号 01-080-1876）。能量色散 X 射线光谱（EDX）证实合成的氧化锌纳米晶体纯度很高。该纳米粒子具有显著的抗氧化活性（自由基清除率高达 97.33%）、有效的抗菌特性以及明显的抗癌活性，在 40 μg/mL 的浓度下对 MCF-7 细胞的抑制率高达 99.75%。这些发现凸显了纳米粒子在健康和医学领域的潜在应用。

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引用次数: 0

Nanopyramid copper structures on screen-printed carbon electrode for high-performance non-enzymatic glucose sensing: A cost-effective and scalable approach 丝网印刷碳电极上的纳米金字塔铜结构用于高性能非酶法葡萄糖传感：一种具有成本效益且可扩展的方法

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-11-01 DOI: 10.1016/j.sbsr.2024.100706

Sahar Bakhshi , Mahsa Rahmanipour , Amir R. Amirsoleimani , Mostafa Rezazadeh , Hossein Siampour , Ahmad Moshaii

We report on the fabrication of a robust non-enzymatic glucose sensor featuring a sensing electrode composed of pyramid-shaped copper/copper oxide (Cu/Cu₂O) nanostructures formed through a simple electrodeposition process on a screen-printed carbon electrode (SPCE). The fabrication of Cu/Cu₂O nanostructures on the SPCE enhances the charge transfer and electrocatalytic performance of the sensor, proving advantageous for glucose sensing. Notably, this morphology contributes to electrochemical glucose determination over a wide linear range of 0.01 to 6 mM, with a sensitivity of 214.04 μA/(mM·cm²) and a low detection limit of 0.03 μM. The proposed simple approach ensures high reproducibility, stable attachment to the printed layer, and cost-effectiveness, making it well-suited for scalable production of non-enzymatic glucose sensors.

我们报告了一种坚固耐用的非酶葡萄糖传感器的制作过程，该传感器的传感电极由金字塔形的铜/氧化铜（Cu/Cu2O）纳米结构组成，这些纳米结构是在丝网印刷碳电极（SPCE）上通过简单的电沉积工艺形成的。在丝网印刷碳电极上制造 Cu/Cu2O 纳米结构增强了传感器的电荷转移和电催化性能，证明了其在葡萄糖传感方面的优势。值得注意的是，这种形态有助于在 0.01 至 6 mM 的宽线性范围内进行电化学葡萄糖测定，灵敏度为 214.04 μA/（mM-cm2），检测限低至 0.03 μM。所提出的简单方法确保了高度的可重复性、印刷层的稳定附着性和成本效益，非常适合于非酶葡萄糖传感器的规模化生产。

引用次数: 0

Design of tunable terahertz metamaterial for variable optical attenuation and sensing applications 设计用于可变光衰减和传感应用的可调太赫兹超材料

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2024-10-25 DOI: 10.1016/j.sbsr.2024.100705

Zonghong Wu , Yu-Sheng Lin

In this work, an actively tunable terahertz metamaterial (TTM) is proposed to realize variable optical attenuation and sensing applications. The unit cell of TTM is composed of H-shaped resonator (HSR) and C-shaped resonator (CSR). The resonant frequency can be tuned from 0.60 THz to 0.82 THz and show an analog electromagnetically induced transparency (EIT) phenomenon. By adjusting the geometry parameters of HSR and CSR, the enhanced quality (Q) factor is obtained from 2 to 14. Moreover, the CSR can be rotated from 0° to 90° to show the potential in the variable optical attenuator (VOA) application. The resonant intensity at 0.60 THz can be gradually decreased and then disappeared eventually when the CSR rotated from 0° to 90° in TE mode. While the resonant intensity at 0.60 THz can be gradually increased and then reach maximum value from 0° to 90° in TM mode. To demonstrate the proposed TTM can be used for the environmental sensing application, the TTM is exposed on the ambient environment with different refractive indexes from 1.0 to 2.2. The maximum sensitivity is 67 GHz. This work offers a novel approach for the THz metamaterial using for the VOA, optical switching, and sensing applications.

本研究提出了一种主动可调太赫兹超材料（TTM），以实现可变光衰减和传感应用。TTM 的单元单元由 H 形谐振器（HSR）和 C 形谐振器（CSR）组成。谐振频率可在 0.60 THz 至 0.82 THz 之间调节，并显示出模拟电磁诱导透明（EIT）现象。通过调整 HSR 和 CSR 的几何参数，可获得从 2 到 14 的增强质量（Q）因子。此外，CSR 还可以从 0° 旋转到 90°，从而显示出在可变光衰减器 (VOA) 应用中的潜力。在 TE 模式下，当 CSR 从 0° 旋转到 90° 时，0.60 THz 处的谐振强度会逐渐减弱，最终消失。而在 TM 模式下，0.60 太赫兹处的谐振强度可逐渐增加，并在 0° 至 90° 之间达到最大值。为了证明所提出的 TTM 可用于环境传感应用，将 TTM 暴露在折射率从 1.0 到 2.2 的不同环境中。最大灵敏度为 67 GHz。这项工作为太赫兹超材料用于 VOA、光开关和传感应用提供了一种新方法。

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引用次数: 0