SARS-CoV-2 surrogate bacteriophage φ6 cross-contamination between fruits and gloves, survival on discarded gloves and inactivation by photodynamic treatment

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2024-09-19 DOI:10.1016/j.fm.2024.104645
Ruthchelly Tavares da Silva , Alyson José dos Santos Franco , Maria Mayara de Souza Grilo , Atila Lima , Karina Lidiane Alcântara Saraiva , Rafaela de Siqueira Ferraz Carvalho , Geany Targino de Souza Pedrosa , Donald W. Schaffner , Marciane Magnani
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Abstract

This study assessed the SARS-CoV-2 surrogate bacteriophage φ6 cross-contamination between high-density polyethylene or polyvinyl chloride gloves and fruits (tomato and cucumber) using different inoculum levels (6.0 and 4.0 log PFU/sample). Bacteriophage φ6 survival on contaminated gloves was assessed over 9 days at 25 °C. The effectiveness of photodynamic treatment using curcumin as a photosensitizer to inactivate φ6 on fruits was determined. The fruit type and the glove material influenced the φ6 transfer. Longer contact times resulted in greater φ6 transfer. The highest φ6 transfer occurred from tomato to HDPE glove (0.8% or −1.1 log % transfer) after 30 s of contact at the higher inoculum level. Bacteriophage φ6 was detected on cross-contaminated HDPE gloves for up to 6 days. Bacteriophage φ6 survived better on vinyl gloves cross-contaminated by cucumber vs. tomato (detected up to 6 vs 3 days). Photodynamic inactivation of φ6 was time-dependent and varied with the tested fruit but was not influenced by viral starting concentration. Photodynamic treatment decreased the φ6 titer by 3.0 and 2.2 log PFU/sample in tomato and cucumber, respectively. Transmission electronic microscopy showed that photodynamic treatment changed the structure of the φ6 capsid. These findings may help in the management of SARS-CoV-2 contamination risks in fruit handling. They may also help in the establishment of effective measures to manage cross-contamination risk.
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SARS-CoV-2 代噬菌体φ6 在水果和手套之间的交叉感染、在废弃手套上的存活以及光动力疗法的灭活作用
本研究使用不同的接种量(6.0 和 4.0 log PFU/样品)评估了高密度聚乙烯或聚氯乙烯手套与水果(西红柿和黄瓜)之间的 SARS-CoV-2 代理噬菌体 φ6 交叉感染。在 25 °C下,评估了噬菌体 φ6 在受污染手套上 9 天的存活率。测定了以姜黄素为光敏剂的光动力疗法灭活水果上φ6的效果。水果类型和手套材料影响了φ6的转移。接触时间越长,φ6 转移越多。在较高接种量下,番茄与高密度聚乙烯手套接触 30 秒后,φ6 转移率最高(0.8% 或-1.1 log %)。在交叉污染的高密度聚乙烯手套上检测到噬菌体 φ6 长达 6 天。在被黄瓜和番茄交叉污染的乙烯基手套上,噬菌体φ6的存活率更高(检测时间分别为6天和3天)。光动力对φ6的灭活作用与时间有关,并随测试水果的不同而变化,但不受病毒起始浓度的影响。光动力处理使番茄和黄瓜中的φ6滴度分别降低了3.0和2.2 log PFU/样品。透射电子显微镜显示,光动力处理改变了φ6包囊的结构。这些发现可能有助于管理水果处理过程中的 SARS-CoV-2 污染风险。这些发现还有助于制定有效措施来管理交叉感染风险。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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