Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2024-09-19 DOI:10.1016/j.stress.2024.100612

Chun-Lan Chen , Di Wu , Qian-Kun Li , Xiao-hu Liu , Xu-Guang Niu , Guo-Xian Zhang , Yong-Yong Zhang , Hui Zhang , Chang-Jie Jiang

{"title":"Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway","authors":"Chun-Lan Chen , Di Wu , Qian-Kun Li , Xiao-hu Liu , Xu-Guang Niu , Guo-Xian Zhang , Yong-Yong Zhang , Hui Zhang , Chang-Jie Jiang","doi":"10.1016/j.stress.2024.100612","DOIUrl":null,"url":null,"abstract":"<div><div>Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na<sub>2</sub>CO<sub>3</sub>), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression (<em>OsBI1</em>) and stress tolerance (<em>OsJRL, OsNAC</em>). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis (<em>OASA1, OASA2</em>) and auxin signaling (<em>Aux/IAA, ARFs</em>). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100612"},"PeriodicalIF":6.8000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002653/pdfft?md5=7cafd528d85383efc51b74cb1a9eea8b&pid=1-s2.0-S2667064X24002653-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X24002653","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na₂CO₃), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression (OsBI1) and stress tolerance (OsJRL, OsNAC). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis (OASA1, OASA2) and auxin signaling (Aux/IAA, ARFs). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

茉莉酸甲酯通过辅酶途径提高水稻对碱性胁迫的耐受性

土壤碱化是全球作物生产面临的一大挑战。本研究揭示了水稻幼苗对碱性胁迫的新型防御机制，其中涉及茉莉酸甲酯（MeJA）和辅助素途径。在碱性胁迫（15 mM Na2CO3）下，水稻幼苗表现出内源 MeJA 水平升高和 JA 响应基因上调。在碱性胁迫下，用 MeJA（50 µM）预处理能显著提高秧苗的存活率和生长速度，并减轻根系损伤。这种处理还能上调与细胞死亡抑制（OsBI1）和胁迫耐受（OsJRL、OsNAC）相关的基因。值得注意的是，MeJA 预处理增加了根中的辅素（吲哚-3-乙酸，IAA）水平，并上调了参与 IAA 合成（OASA1、OASA2）和辅素信号转导（Aux/IAA、ARFs）的基因。用 N-1-naphthylphthalamic acid 阻断辅素运输会加剧根系在碱性胁迫下的损伤，并削弱 MeJA 的保护作用。这些结果突显了 MeJA 诱导的辅助素通路激活在增强水稻对碱性胁迫的耐受性中的关键作用，并为制定提高作物在碱性土壤中的抗逆性的策略提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.