Plant Stress最新文献

{"title":"The dynamic transcriptome reveals response patterns to black shank disease in tobacco (Nicotiana tabacum L.)","authors":"Zhijun Tong ,&nbsp;Zuoqian Fan ,&nbsp;Tianyu Du ,&nbsp;Dunhuang Fang ,&nbsp;Xueyi Sui ,&nbsp;Chuyu Ye ,&nbsp;Qian-Hao Zhu ,&nbsp;Longjiang Fan ,&nbsp;Bingguang Xiao ,&nbsp;Enhui Shen","doi":"10.1016/j.stress.2024.100676","DOIUrl":"10.1016/j.stress.2024.100676","url":null,"abstract":"<div><div>Black shank disease, caused by <em>Phytophthora nicotianae</em>, is one of the major causes of yield loss in tobacco production. The present study aimed to explore the dynamic transcriptome in tobacco genotypes resistant or susceptible to black shank disease and to understand the defense response of tobacco to <em>P. nicotianae</em> infection. Roots and stems were sampled from two resistant and two susceptible materials at 0, 12, 24, 48, and 72 h post infection and used in RNA-sequencing. Conventional approaches that identify differentially expressed genes are not the best way for handling the complex datasets, so a new method that calculates the standard deviation among samples was applied to identify transcripts with variable expression levels in roots and stems of the four materials at different time points. Of the total of 229,501 transcripts, 7,261 were found to be variable transcripts, with many of them annotated to be related to defense responses against pathogen infection. These variable transcripts showed expression patterns that varied significantly between roots and stems as well as between the resistant and the susceptible materials. Several transcripts were identified to be potential candidates for further functional characterization. Our findings provide invaluable insights into the dynamic transcriptome in defense responses of tobacco against <em>P. nicotianae</em> infection.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100676"},"PeriodicalIF":6.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-mediated oxidative stress and its scavenging differ between citrus hybrids with medium and late fruit maturation","authors":"Jin Jia,&nbsp;Mingjiong Zhao,&nbsp;Rui Liu,&nbsp;Caixin Xue,&nbsp;Zhuyuan Xia,&nbsp;Bin Hu,&nbsp;Heinz Rennenberg","doi":"10.1016/j.stress.2024.100670","DOIUrl":"10.1016/j.stress.2024.100670","url":null,"abstract":"<div><div>Drought stress is a major environmental factor limiting citrus productivity. Still, differences in drought sensitivity between citrus hybrids of different maturation periods have so far not been reported. Here, we selected a medium-maturing (Fertile orange: FO (<em>Citrus reticulata</em> cv. Fertile orange) and a late-maturing citrus hybrid (Newhall Navel orange: NO (<em>Citrus sinensis</em> Osbeck cv. Newhall) and determined the physiological and biochemical traits of leaves, roots, wood and bark. Our results showed that drought significantly decreased net photosynthetic rate (<em>Pn</em>), stomatal conductance (<em>Gs</em>) and transpiration rate (<em>Tr</em>) of citrus leaves. Oxidative stress upon drought was indicated by enhanced foliar malondialdehyde (MDA) and hydrogen peroxide contents, as well as a stimulation of the anti-oxidative system. This stimulation included the contents of dehydroascorbic acid (DHA), glutathione (GSH) and oxidized glutathione (GSSG) in leaves, roots, wood and bark, as well as activities of antioxidative enzymes of glutathione reductase (GR), dehydroascorbate reductase (DHAR), superoxide dismutase (SOD) and peroxidase (POD). The late maturing NO hybrid not only showed better general physiological performance as indicated by increased <em>Pn</em> in leaves, but also higher biochemical ROS scavenging and osmotic capacity as indicated by increased ascorbic acids (ASA), DHA, and proline contents, as well as activities of enzymes of SOD, POD, ASA/DHA and GSH/GSSG ratios in the investigated tissues compared to the FO hybrid under drought and control conditions. Analysis of molecular mechanisms of signaling, regulatory and functional genes expression are suggested for future studies to elucidate the complex interplay of molecular, biochemical and physiological responses of citrus hybrids to drought.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100670"},"PeriodicalIF":6.8,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histochemical and gene expression changes in Cannabis sativa hypocotyls exposed to increasing concentrations of cadmium and zinc","authors":"Roberto Berni ,&nbsp;Jean-Francois Hausman ,&nbsp;Stanley Lutts ,&nbsp;Gea Guerriero","doi":"10.1016/j.stress.2024.100668","DOIUrl":"10.1016/j.stress.2024.100668","url":null,"abstract":"<div><div>Hemp (<em>Cannabis sativa</em> L.) is a versatile crop that produces cellulosic bast fibres used in textiles and biocomposites. Is also finds use in phytoremediation, being a good candidate for the cultivation on marginal lands, such as those contaminated by heavy metals (HMs). HMs like cadmium (Cd) and zinc (Zn) are known to affect plant growth and impair the biosynthesis of cellulose and lignin at the cell wall level. Since cellulose is the major component in the gelatinous layer of bast fibres, HMs can impact the structure of hemp fibres and, consequently, their mechanical properties. This study investigates how varying concentrations of Cd and Zn in the soil affect the bast fibres of hemp plantlets. The chosen model is the hypocotyl, as it is ideal for studying bast fibre development: it exhibits a temporal separation between the elongation and thickening phases within a short period of approximately three weeks. <em>C. sativa</em> plantlets were grown for 20 days, and the hypocotyls sampled to perform histochemical observations, gene expression analysis, as well as to quantify biomass yield and Cd/Zn accumulation. Hemp plantlets grown in soils with the three highest Zn concentrations were smaller than the control group, whereas no decrease in size was observed under elevated Cd concentrations. However, at the highest Cd concentration, the root system exhibited enhanced development, accompanied by a significant increase in dry weight across all the concentrations tested. The quantification of Cd and Zn showed that the roots were the main organs accumulating HMs. Cd at the two highest concentrations decreased significantly the lumen area of bast fibres and increased their cell wall thickness. Zn decreased significantly the lumen area, but it did not impact the thickness of the cell wall at the highest concentration. Cd also increased the number of secondary fibres. Immunohistochemistry highlighted a different pattern of crystalline cellulose distribution with a signal that was less homogeneous in the presence of Cd and Zn. Gene expression analysis revealed changes in transcripts encoding cellulose synthases, fasciclin-like arabinogalactan proteins, class III peroxidases. The results obtained shed light on the molecular response and bast fibre histological changes occurring in young hemp plants exposed to Cd and Zn.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100668"},"PeriodicalIF":6.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological mechanisms regulating source-sink interactions and grain yield formation in heat-stressed wheat","authors":"Najeeb Ullah,&nbsp;Malik Adil Nawaz,&nbsp;Mohammed Alsafran","doi":"10.1016/j.stress.2024.100654","DOIUrl":"10.1016/j.stress.2024.100654","url":null,"abstract":"<div><div>Climate change and increasing atmospheric temperatures significantly challenge global wheat productivity and food security. Unpredictable weather patterns and frequent heatwaves, particularly during reproductive and grain-filling phases of wheat crops significantly reduce grain yield and quality. This review examines current literature on the impact of heat intensity and duration on grain yield components during these sensitive growth phases. Using the published literature, we quantified grain yield losses in response to varying heat intensity and duration during different developmental phases of wheat crops. The data suggest that grain number loss in wheat is poorly correlated with heat intensity and timing (0 to 15 days before anthesis) alone but it strongly responds (<em>r</em>²=0.45) to the number of hot days, with a 0.2 % loss of grains for each additional hot day with a temperature above optimum (16–22 °C). Further, for every 1 °C increase in mean temperature above optimum during sensitive phases (from -5 to 15 days since anthesis), individual grain weight decreases by approximately 2.1 %. This review also discusses how changes in source-sink regulation, particularly carbon assimilation, storage, transport and sugar metabolism in wheat under terminal heat are associated with grain yield losses. It also identifies the research gaps in heat wheat interaction, discussing potential opportunities (e.g., breeding and management) for sustaining wheat production under future hot environments.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100654"},"PeriodicalIF":6.8,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roots of resistance: Unraveling microbiome-driven plant immunity","authors":"Dhananjaya Pratap Singh ,&nbsp;Sudarshan Maurya ,&nbsp;Lovkush Satnami ,&nbsp;Renu ,&nbsp;Ratna Prabha ,&nbsp;Birinchi K. Sarma ,&nbsp;Nagendra Rai","doi":"10.1016/j.stress.2024.100661","DOIUrl":"10.1016/j.stress.2024.100661","url":null,"abstract":"<div><div>The intricate interplay between microbiome and plant immunity represents a frontier in plant biology with significant implications for agriculture and ecosystem management. This review explores intricate relationship between plant immunity and the microbiome, highlighting its significance in addressing current agricultural and environmental challenges. The plant immune system, comprising pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), plays crucial role in shaping microbial communities in the rhizosphere. Phytohormones such as salicylic acid, jasmonic acid, and ethylene are the key modulators of plant defenses and contribute to rhizosphere microbiome composition. The concept of defense priming and plant immune memory emerges as a promising avenue for enhancing crop resilience against phytopathogens and environmental stresses. Root exudates and plant defense signatures actively influence rhizosphere microbiome structure, establishing a bidirectional relationship between plants and their microbial partners. This interaction is particularly relevant in the context of climate change, where plants face increasing biotic and abiotic stresses. Understanding and leveraging these complex interactions holds promise for developing more sustainable agricultural practices, reducing reliance on chemical inputs, and ensuring food security in the face of global challenges. We have stressed upon the importance of viewing the plant-soil-microbiome system as an integrated unit or holobiont. As agriculture grapples with the challenges of feeding a growing population under changing environmental conditions, harnessing the power of plant-microbiome interactions presents a promising strategy for improving food security and promoting ecosystem health.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100661"},"PeriodicalIF":6.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic and transcriptomic analyses reveal MYB-Related genes involved in drought resistance in grafted potatoes via the flavonoid pathway","authors":"Yinqiao Jian ,&nbsp;Chunyan Gao ,&nbsp;Yangyang Shang ,&nbsp;Junhong Qin,&nbsp;Shaoguang Duan,&nbsp;Chunsong Bian,&nbsp;Guangcun Li","doi":"10.1016/j.stress.2024.100665","DOIUrl":"10.1016/j.stress.2024.100665","url":null,"abstract":"<div><div>Drought poses a significant challenge to global potato production. Grafting, a classical horticultural technique, has the potential to enhance resistance to both biotic and abiotic stresses. However, the use of grafting to improve drought resistance in potatoes, along with the underlying genetic and regulatory changes remains inadequately documented. In this study, we investigated the drought phenotypes, as well as the metabolomic and transcriptomic profiles of leaves and roots in self-grafted (drought-sensitive scion/ drought-sensitive rootstock, SS) and hetero-grafted (drought-sensitive scion/drought-tolerant rootstock, ST) potatoes. After 40 days, hetero-grafts exhibited greater drought resistance as well as lower dry matter content and higher soluble sugar content compared to self-grafts, indicating that grafting with drought-tolerant rootstocks can enhance the drought resistance of the scion and revealed physiological process. Metabolomic analysis revealed a significant enrichment of flavonoids, particularly in comparisons between SS-leaf vs. ST-leaf and SS-root vs. ST-root. Transcriptomic analysis further supported these findings, showing an enrichment in the biosynthesis of plant secondary metabolites in the same comparisons, aligning with metabolomic data. These differentially accumulated metabolites and expressed genes, particularly in SS-leaf vs. ST-leaf comparison, suggest a mechanism involving long-distance metabolites and mRNA in grafting-mediated drought resistance. Weighted Gene Co-expression Network Analysis identified the yellow module, which correlated with drought, and highlighted MYB or MYB-related genes as hub genes. Our results reveal global metabolomic and transcriptomic features associated with drought tolerance in potatoes, demonstrating that grafting can alter the composition and accumulation of genes and metabolites, leading to enhanced drought resistance. The significant role of flavonoids as modulators of drought resistance, supported by comprehensive transcriptomic and metabolomic analyses, underscores the pivotal regulatory function of the MYB-WD40-bHLH transcription factor complex in orchestrating the stress response.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100665"},"PeriodicalIF":6.8,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic identification and expression profiling of calmodulin-binding transcription activator genes reveal insights into their functional diversity against pathogens in sugarcane","authors":"Talha Javed ,&nbsp;Wenzhi Wang ,&nbsp;Juan Li ,&nbsp;Tingting Sun ,&nbsp;Linbo Shen ,&nbsp;San-Ji Gao ,&nbsp;Shuzhen Zhang","doi":"10.1016/j.stress.2024.100660","DOIUrl":"10.1016/j.stress.2024.100660","url":null,"abstract":"<div><div>The calmodulin-binding transcription activator (CAMTA) proteins in plants play significant roles in signal-transduction, activation of regulatory networks, and defense response against environmental stressors. In this study we systematically identified a total 17 CAMTA genes (named as <em>SsnpCAMTAs</em>) in <em>Saccharum spontaneum</em> Np-X genome. Moreover, <em>SsnpCAMTAs</em> exhibited diverse physio-chemical and gene structural attributes. Notably, eight <em>SsnpCAMTA</em> gene pairs displayed segmental duplication events, while CAMTA genes from <em>S. spontaneum</em> Np-X and <em>Arabidopsis thaliana</em> shared homology relationship. In-vitro interaction networking showed that SsnpCAMTA1 and SsnpCAMTA2 were the core protein which interacted with each other as well as with other four different proteins (SsnpCAMTA5/7/13/17). To check functional variability, the expression profiles of <em>SsnpCAMTAs</em> were quantified in transcriptomic and proteomic datasets triggered by <em>Xanthomonas albilineans</em> (<em>Xa</em>) and <em>Acidovorax avenae</em> subsp. <em>avenae</em> (<em>Aaa</em>). The temporal expression of <em>SsnpCAMTA4/5</em> was ranged between 1.7–3.5 folds in sugarcane cultivars triggered by <em>Xa</em> infection. Most of the <em>SsnpCAMTAs</em> exhibited irregular expression profile in sugarcane cultivars triggered by <em>Aaa</em> infection. Additionally, transcript expression profiles of eight candidate genes <em>SsnpCAMTA1/2/5/7/8/12/13/17</em> were determined by RT-qPCR assay in sugarcane cultivars Zhongtang3 (resistant to smut) and ROC22 (susceptible to smut) under <em>Sporisorium scitamineum</em> pathogen infection. Interestingly, the transcript expression of <em>SsnpCAMTA5</em> was upregulated from 1.4 to 10.8 folds as compared to control in both cultivars, while <em>SsnpCAMTA8</em> was downregulated in both cultivars. Overall, our results provide valuable candidate gene resources for the development of disease-resistant sugarcane cultivars in the face of current climate change scenarios.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100660"},"PeriodicalIF":6.8,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of groundwater depth and tree age on the non-structural carbohydrates of Haloxylon ammodendron","authors":"Lan Peng ,&nbsp;Guangyou Hao ,&nbsp;Hui Liu ,&nbsp;Hui Shen ,&nbsp;Xiaobing Zhou ,&nbsp;Yongxin Zang ,&nbsp;Jing Zhang ,&nbsp;Yuanming Zhang","doi":"10.1016/j.stress.2024.100659","DOIUrl":"10.1016/j.stress.2024.100659","url":null,"abstract":"<div><div>Nonstructural carbohydrates (NSC) are important substrates for plant growth and metabolism, and their concentration reflects the plant's ability to adapt to environmental changes. Although the response of NSC to changes in water availability has been extensively studied, it is still not fully understood whether this response is modulated by tree ages and organs. This study investigates <em>Haloxylon ammodendron</em> (C.A. Mey.) Bunge ex Fenzl, the dominant species in the Gurbantunggut Desert in the Uyghur Autonomous Region of China. Utilizing the natural topographic conditions characterized by a gradual increase in groundwater depth from the desert edge to the hinterland, we collected samples of different age classes of <em>H. ammodendron</em> along a groundwater depth gradient of 3, 7, 10, and 14 m. We measured the total concentrations of non-structural carbohydrates (NSC) and its components soluble sugar (SS) and starch (ST) in the assimilative twigs and stems. The results showed that the assimilative twigs of <em>H. ammodendron</em> exhibited higher NSC concentrations at the site with the deepest groundwater, while the other three sites showed similar NSC concentrations. Furthermore, as groundwater depth increased, the concentrations of SS in the assimilative twigs increased, whereas ST concentrations decreased. Similarly, the concentrations of SS in the stems also increased at sites with deeper groundwater. The NSC concentrations in the assimilative twigs were significantly affected by groundwater depth, while variations in stem NSC were primarily driven by plant age. In younger trees, higher soluble sugars concentrations in the stem may enhance water transport efficiency, whereas older trees tend to store more NSC to alleviate drought stress. Overall, elevated nonstructural carbohydrate concentrations contributed to greater drought resilience in <em>H. ammodendron</em>. These results suggest that different age classes of <em>H. ammodendron</em> exhibit distinct physiological responses to decreasing groundwater depth. The varying requirements for soluble sugars and starch in <em>H. ammodendron</em> help to partially mitigate the adverse effects of reduced groundwater accessibility. These findings provide important insights into the physiological adaptations of <em>H. ammodendron</em> in arid environments and offer a scientific basis for future ecological restoration and management strategies.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100659"},"PeriodicalIF":6.8,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intercropping grapevine with rosemary: A promising alternative to conventional agriculture in the current frame of global change","authors":"Sílvia Campillos ,&nbsp;Camila Ribalta-Pizarro ,&nbsp;Laia Jené ,&nbsp;Paula Muñoz ,&nbsp;Sergi Munné-Bosch","doi":"10.1016/j.stress.2024.100662","DOIUrl":"10.1016/j.stress.2024.100662","url":null,"abstract":"<div><div>The Mediterranean region is well known for its long history of viticulture and the use of herbs and spices in its cuisine. Given the increasing focus on intercropping as a sustainable agricultural approach to maximize land use efficiency or improve soil fertility amid global change, we conducted a study to assess the feasibility of intercropping grapevine (<em>Vitis vinifera</em> L. cv. Merlot) with rosemary (<em>Salvia rosmarinus</em> Spenn.) in a Mediterranean vineyard. The trial was established in a 22-year-old vineyard, where rows of rosemary plants were cultivated between grapevine rows one year prior to the evaluation. To test intercropping feasibility and the abiotic stress effects on the harvest of rosemary, two sampling dates were set to monitor grape maturation (<em>veraison</em> and harvest) and rosemary quality in terms of antioxidant contents and composition, so that both crops were simultaneously analyzed under typical Mediterranean conditions, using both physiological and quality parameters. Results showed that intercropping with young rosemary plants did not affect grapevine physiology state, and observed differences in the two samplings were due to environmental stress only, indicating a positive coexistence between these crops in the context of global change. Regarding grape quality, variations in total soluble sugars, titratable acidity, and phenolic compounds were influenced by ripening during the summer, but not by intercropping, thus maintaining fruit quality. Rosemary contained high levels of antioxidants and exhibited strong antioxidant activity, particularly during mid-August (coinciding with vintage), indicating its potential as a valuable source of natural antioxidants for dietary intake, including phenolic diterpenes, vitamin C, and vitamin E. In conclusion, intercropping grapevines with rosemary did not negatively impact plant physiological performance or grape quality, promoting the production of two high-quality products with significant added value in the food sector under current stressful Mediterranean climatic conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100662"},"PeriodicalIF":6.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis of B. oleracea L. var. italica and B. macrocarpa Guss. genotypes under drought stress: de novo vs reference genome assembly","authors":"Grete Francesca Privitera ,&nbsp;Simone Treccarichi ,&nbsp;Roberta Nicotra ,&nbsp;Ferdinando Branca ,&nbsp;Alfredo Pulvirenti ,&nbsp;Angela Roberta Lo Piero ,&nbsp;Angelo Sicilia","doi":"10.1016/j.stress.2024.100657","DOIUrl":"10.1016/j.stress.2024.100657","url":null,"abstract":"<div><div>Drought stress influences plant growth and development affecting some physiological processes during the growing cycle. The improvement of the drought stress resistance of the plant will allow the reduction of the water requirements of the crops, representing the new frontier for agriculture, as consequence of the ongoing climatic changes. The individuation of genetic features useful for enhancing the reduction of water use and the plant drought resistance will be one of the key strategy for providing enough food for the foreseen increment of the global population. Nowadays, the cultivars used for modern agriculture show a narrow genetic diversity due to the domestication process carried out of each crop, resulting with a less adaptation to the environmental conditions affected by both antropic activities and the climatic change in act. Among the several grown species, <em>Brassica oleracea</em> L. (<em>n</em> = 9) crops are particularly vulnerable to the adverse effects of water deficiency. Transcriptomic analysis is a powerful tool that allows researchers to identify genes and pathways, that are activated or repressed in response to each specific stress, elucidating the complex regulatory networks that underlie the correspondent response. To individuate the differentially expressed genes we compared two drought-sensitive <em>B. oleracea</em> L. var. <em>botritis x italica</em> Sicilian landraces and two drought-tolerant <em>B. macrocarpa</em> Guss populations grown in ordinary irrigation regimes and in water deficit conditions. Their transcriptome was obtained by a RNAseq approach. The individuated sensible and tolerant drought stress genotypes showed significant difference for H₂O₂ content, leaf area and SPAD index (Soil Plant Analysis Development). For better identify crucial genes and pathways associated with drought stress response of both the sensible and tolerant genotypes a thorough evaluation of both <em>de novo</em> assembly and reference <em>B. oleracea</em> var. <em>italica</em> genome-based assembly was conducted. The comparison of the transcriptomes allowed to identify the crucial genes and pathways associated with tolerance to drought stress in <em>Brassica oleracea</em> L. crops. As main results, we individuated one gene coding transcription factor showing opposite behavior in the sensitive and tolerant signatures. The adoption of two transcriptome assembly methods provided a more comprehensive dataset, enabling an unbiased interpretation of the outcomes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100657"},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}