Plant Stress最新文献

Optimal rooting substrates and hormonal regulation via a multi-omics analysis during Vitis davidii cutting rooting

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-08 DOI: 10.1016/j.stress.2025.100851

Ting Zheng , Lingzhu Wei , Jiang Xiang , Weiwei Zheng , Jiang Wu , Jianhui Cheng

Spine grapes (Vitis davidii Foex), a typical wild grape species native to China, is primarily propagated through cutting. However, successful rooting remains a significant challenge in production. Thus, one aim of this study is to identify an optimal substrate for rooting of V.davidii cuttings and explore the hormonal regulation under the rooting process. Among 13 substrates tested, T12 (perlite) produced the highest rooting rate (90%) and a 100% callus formation rate, followed by T1 (rice husk biochar + coarse river sand 1:1) and T2 (rice husk biochar + perlite 1:1). Rooting materials with large, hard particles, such as perlite and coarse river sand, improved rooting. Electron microscopy showed that V. davidii exhibited mixed-type rooting, and there was no direct relationship between callus formation and rooting success. Transcriptome and metabolome analyses indicated that significant differences in auxin and cytokinin were observed between P1 vs P2, suggesting their important roles in bud germination and leaf expansion. Salicylic acid (SA) was essential for callus and root formation, while jasmonic acid (JA) and gibberellins (GA) were more closely associated with direct rooting of cuttings rather than callus formation. Integrated Gene Ontology (GO) and KEGG analyses screened 17 crucial hormone regulatory transcription factors during rooting in cuttings. Among these, ARR18 and RR26 were mainly expressed at the P1 stage, while TIFY6B was predominant in P2, and GAI1 was highly active in the P1 and P4 stages. Remarkably, TIFY10A expression was 2.93 times higher in P3 and P4 compared to P1 and P2 and was highly correlated with various hormones. TIFY10A expression increased sharply under exogenous JA treatment and exhibited tissue-specificity. These findings suggest an important role of TIFY10A in the rooting process of grape V. davidii cuttings, particularly in callus and adventitious root formation.

{"title":"Optimal rooting substrates and hormonal regulation via a multi-omics analysis during Vitis davidii cutting rooting","authors":"Ting Zheng , Lingzhu Wei , Jiang Xiang , Weiwei Zheng , Jiang Wu , Jianhui Cheng","doi":"10.1016/j.stress.2025.100851","DOIUrl":"10.1016/j.stress.2025.100851","url":null,"abstract":"<div><div>Spine grapes (<em>Vitis davidii</em> Foex), a typical wild grape species native to China, is primarily propagated through cutting. However, successful rooting remains a significant challenge in production. Thus, one aim of this study is to identify an optimal substrate for rooting of <em>V.davidii</em> cuttings and explore the hormonal regulation under the rooting process. Among 13 substrates tested, T12 (perlite) produced the highest rooting rate (90%) and a 100% callus formation rate, followed by T1 (rice husk biochar + coarse river sand 1:1) and T2 (rice husk biochar + perlite 1:1). Rooting materials with large, hard particles, such as perlite and coarse river sand, improved rooting. Electron microscopy showed that <em>V. davidii</em> exhibited mixed-type rooting, and there was no direct relationship between callus formation and rooting success. Transcriptome and metabolome analyses indicated that significant differences in auxin and cytokinin were observed between P1 vs P2, suggesting their important roles in bud germination and leaf expansion. Salicylic acid (SA) was essential for callus and root formation, while jasmonic acid (JA) and gibberellins (GA) were more closely associated with direct rooting of cuttings rather than callus formation. Integrated Gene Ontology (GO) and KEGG analyses screened 17 crucial hormone regulatory transcription factors during rooting in cuttings. Among these, ARR18 and RR26 were mainly expressed at the P1 stage, while TIFY6B was predominant in P2, and GAI1 was highly active in the P1 and P4 stages. Remarkably, TIFY10A expression was 2.93 times higher in P3 and P4 compared to P1 and P2 and was highly correlated with various hormones. TIFY10A expression increased sharply under exogenous JA treatment and exhibited tissue-specificity. These findings suggest an important role of TIFY10A in the rooting process of grape <em>V. davidii</em> cuttings, particularly in callus and adventitious root formation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100851"},"PeriodicalIF":6.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lime-induced iron deficiency stimulates a stronger response in tolerant grapevine rootstocks compared to low iron availability

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-06 DOI: 10.1016/j.stress.2025.100841

Sarhan Khalil , Rebeka Strah , Arianna Lodovici , Petr Vojta , Jörg Ziegler , Maruša Pompe Novak , Laura Zanin , Nicola Tomasi , Astrid Forneck , Michaela Griesser

Iron (Fe) is abundant in soil, but its bioavailability can be limited by environmental factors, negatively impacting plant growth and productivity. While root mechanisms for enhancing Fe uptake are well-studied in some model plants, the responses of tolerant and susceptible grapevine rootstocks to low Fe availability remain poorly understood. This study examined the responses of two grapevine rootstocks, Fercal (tolerant) and 3309C (susceptible), to three Fe conditions: direct Fe deficiency (−Fe), induced Fe deficiency through the addition of bicarbonate (+Fe+BIC), and control (+Fe). Our main findings include: 1) more severe leaf symptoms in 3309C than in Fercal independent of the type of stress, 2) overall growth reduction due to direct Fe deficiency (−Fe), while under induced Fe deficiency (+Fe+BIC) Fercal strongly increased root biomass. This observation is supported by the increased expression of root-development related genes VviSAUR66 and VviZAT6, 3) enhanced organic acid contents under induced Fe deficiency (+Fe+BIC) and different organic acids profiles depending on applied stress and genotype, and 4) stronger modulation of gene expression in Fercal root tips, including enhanced expression of Fe mobilization and transport genes (VviOPT3, VviIREG3, VviZIF1). Overall, bicarbonate-induced Fe deficiency (+Fe+BIC) had greater negative effects than direct Fe deficiency (−Fe), with Fercal showing a higher adaptive capability to maintain Fe homeostasis.

{"title":"Lime-induced iron deficiency stimulates a stronger response in tolerant grapevine rootstocks compared to low iron availability","authors":"Sarhan Khalil , Rebeka Strah , Arianna Lodovici , Petr Vojta , Jörg Ziegler , Maruša Pompe Novak , Laura Zanin , Nicola Tomasi , Astrid Forneck , Michaela Griesser","doi":"10.1016/j.stress.2025.100841","DOIUrl":"10.1016/j.stress.2025.100841","url":null,"abstract":"<div><div>Iron (Fe) is abundant in soil, but its bioavailability can be limited by environmental factors, negatively impacting plant growth and productivity. While root mechanisms for enhancing Fe uptake are well-studied in some model plants, the responses of tolerant and susceptible grapevine rootstocks to low Fe availability remain poorly understood. This study examined the responses of two grapevine rootstocks, Fercal (tolerant) and 3309C (susceptible), to three Fe conditions: direct Fe deficiency (−Fe), induced Fe deficiency through the addition of bicarbonate (+Fe+BIC), and control (+Fe). Our main findings include: 1) more severe leaf symptoms in 3309C than in Fercal independent of the type of stress, 2) overall growth reduction due to direct Fe deficiency (−Fe), while under induced Fe deficiency (+Fe+BIC) Fercal strongly increased root biomass. This observation is supported by the increased expression of root-development related genes <em>VviSAUR66</em> and <em>VviZAT6,</em> 3) enhanced organic acid contents under induced Fe deficiency (+Fe+BIC) and different organic acids profiles depending on applied stress and genotype, and 4) stronger modulation of gene expression in Fercal root tips, including enhanced expression of Fe mobilization and transport genes (<em>VviOPT3, VviIREG3, VviZIF1</em>). Overall, bicarbonate-induced Fe deficiency (+Fe+BIC) had greater negative effects than direct Fe deficiency (−Fe), with Fercal showing a higher adaptive capability to maintain Fe homeostasis.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100841"},"PeriodicalIF":6.8,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf Development and Its Interaction with Phyllospheric Microorganisms: Impacts on Plant Stress Responses

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-06 DOI: 10.1016/j.stress.2025.100843

Huanhuan Yang, Jing Liu, Mei Ma, Zilong Tan, Kaiyue Zhang, Ruiqi Sun, Xinxin Zhan, Dayong Cui

Leaf development is regulated by intricately genetic and hormonal networks, which are further modulated by environmental inputs. This study provides a detailed review of the morphogenesis and molecular processes involved in leaf development and contrast the distinct pathways in monocotyledons and dicotyledons. We focus on the initiation patterns and venation architectures, as well as the key cellular and molecular mechanisms that regulate these developmental processes. We analyze the interactions between these microorganisms and host plants, emphasizing their influence on nutrient cycling, hormonal balance, and plant health. The research systematically evaluates the effects of several environmental stresses, salt, drought, temperature extremes, and heavy metal exposure on leaf development and phyllospheric microbial communities. These stresses induce specific adaptive morphological and physiological responses in leaves, such as modifications in leaf size, thickness, venation, and surface characteristics, which are crucial for plant survival and efficiency. Our findings elucidate the dynamic interactions between plants and phyllospheric microorganisms, highlighting their essential roles in enhancing plant resilience to environmental stresses. This study not only advances our understanding of leaf development and plant-microbe interactions but also provides insights into potential agricultural applications where microbial management could enhance crop tolerance and production under environmental stress.

{"title":"Leaf Development and Its Interaction with Phyllospheric Microorganisms: Impacts on Plant Stress Responses","authors":"Huanhuan Yang, Jing Liu, Mei Ma, Zilong Tan, Kaiyue Zhang, Ruiqi Sun, Xinxin Zhan, Dayong Cui","doi":"10.1016/j.stress.2025.100843","DOIUrl":"10.1016/j.stress.2025.100843","url":null,"abstract":"<div><div>Leaf development is regulated by intricately genetic and hormonal networks, which are further modulated by environmental inputs. This study provides a detailed review of the morphogenesis and molecular processes involved in leaf development and contrast the distinct pathways in monocotyledons and dicotyledons. We focus on the initiation patterns and venation architectures, as well as the key cellular and molecular mechanisms that regulate these developmental processes. We analyze the interactions between these microorganisms and host plants, emphasizing their influence on nutrient cycling, hormonal balance, and plant health. The research systematically evaluates the effects of several environmental stresses, salt, drought, temperature extremes, and heavy metal exposure on leaf development and phyllospheric microbial communities. These stresses induce specific adaptive morphological and physiological responses in leaves, such as modifications in leaf size, thickness, venation, and surface characteristics, which are crucial for plant survival and efficiency. Our findings elucidate the dynamic interactions between plants and phyllospheric microorganisms, highlighting their essential roles in enhancing plant resilience to environmental stresses. This study not only advances our understanding of leaf development and plant-microbe interactions but also provides insights into potential agricultural applications where microbial management could enhance crop tolerance and production under environmental stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100843"},"PeriodicalIF":6.8,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic understanding of GABA and trehalose in modulating plant response to drought stress

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-05 DOI: 10.1016/j.stress.2025.100838

Ambika Choudhary , Eugenie Nepovimova , Vishnu D. Rajput , Tabarak Malik , Monika Choudhary , Nidhi Bhardwaj , Lukas Peter , Sunil Puri , Neetika Kimta

Drought stress is one of the most critical environmental factors that hinders plant growth, productivity, and survival worldwide. This review presents the detrimental effects of drought stress on plant growth, its development, and key physical, physiological, and biochemical traits. It is also reviewing effective strategies related to drought management to alleviate these impacts. In fact, plants employ various physiological and biochemical mechanisms to counteract drought stress, with γ-aminobutyric acid (GABA) and trehalose emerging as significant masters of drought tolerance. GABA is an amino acid that isn't found in proteins. It is an important osmoprotectant, antioxidant, and signaling molecule that changes how stress-responsive pathways work. It enhances photosynthetic efficiency, regulates reactive oxygen species (ROS), and stabilizes cellular structures. Similarly, trehalose, a non-reducing disaccharide, acts as a crucial osmolyte, protecting plants from dehydration by stabilizing proteins and membranes, decreasing oxidative damage, and enhancing metabolic efficiency. Both molecules play essential roles in stress-related gene regulation, scavenging of ROS, and maintaining homeostasis of cellular environment under drought conditions. Lastly, reviews also highlight the current knowledge on the biosynthesis and metabolism of GABA and trehalose, emphasizing their potential applications in improving drought resilience in crops through genetic modification and exogenous application. Furthermore, it underscores their value of these two components in helping plants withstand harsh environmental challenges and lessen the adverse effects of abiotic stress, i.e., drought stress. Understanding these mechanisms provides valuable insights for enhancing plant performance in water-limited environments.

{"title":"Mechanistic understanding of GABA and trehalose in modulating plant response to drought stress","authors":"Ambika Choudhary , Eugenie Nepovimova , Vishnu D. Rajput , Tabarak Malik , Monika Choudhary , Nidhi Bhardwaj , Lukas Peter , Sunil Puri , Neetika Kimta","doi":"10.1016/j.stress.2025.100838","DOIUrl":"10.1016/j.stress.2025.100838","url":null,"abstract":"<div><div>Drought stress is one of the most critical environmental factors that hinders plant growth, productivity, and survival worldwide. This review presents the detrimental effects of drought stress on plant growth, its development, and key physical, physiological, and biochemical traits. It is also reviewing effective strategies related to drought management to alleviate these impacts. In fact, plants employ various physiological and biochemical mechanisms to counteract drought stress, with γ-aminobutyric acid (GABA) and trehalose emerging as significant masters of drought tolerance. GABA is an amino acid that isn't found in proteins. It is an important osmoprotectant, antioxidant, and signaling molecule that changes how stress-responsive pathways work. It enhances photosynthetic efficiency, regulates reactive oxygen species (ROS), and stabilizes cellular structures. Similarly, trehalose, a non-reducing disaccharide, acts as a crucial osmolyte, protecting plants from dehydration by stabilizing proteins and membranes, decreasing oxidative damage, and enhancing metabolic efficiency. Both molecules play essential roles in stress-related gene regulation, scavenging of ROS, and maintaining homeostasis of cellular environment under drought conditions. Lastly, reviews also highlight the current knowledge on the biosynthesis and metabolism of GABA and trehalose, emphasizing their potential applications in improving drought resilience in crops through genetic modification and exogenous application. Furthermore, it underscores their value of these two components in helping plants withstand harsh environmental challenges and lessen the adverse effects of abiotic stress, i.e., drought stress. Understanding these mechanisms provides valuable insights for enhancing plant performance in water-limited environments.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100838"},"PeriodicalIF":6.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating salt stress in maize using Ecklonia maxima seaweed extracts

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-03 DOI: 10.1016/j.stress.2025.100828

B.C. Pienaar , B.M. Majeke , M.F. Wittenberg , A.E. Adetunji , L. Nephali , F. Tugizimana , M.S. Rafudeen

Salinity stress poses a significant threat to crop productivity, making it crucial to explore strategies that alleviate its adverse effects. This study investigated the impact of Afrikelp®, a commercial seaweed extract (SE) biostimulant, derived from Ecklonia maxima on maize (Zea mays L.) plants subjected to salinity stress. Gas exchange analysis revealed that plants treated with SE Afrikelp® exhibited significantly higher photosynthetic rates under control conditions (45 % respectively) and 200 mM salt stress (95 % respectively) compared to untreated plants. Similarly, plants treated with SE Afrikelp® and 200 mM salt stress had significantly higher internal CO₂ concentrations and transpiration rates compared to untreated salt stressed plants. Morphological analysis demonstrated that SE Afrikelp® enhanced plant growth and reduced electrolyte leakage under both control and salinity stress conditions. Metabolomic profiling revealed significant alterations in primary metabolites, particularly amino acids, sugars, and organic acids. As such, the results reveal that SE Afrikelp® induced a metabolic reprogramming towards stress alleviation and enhanced defences. Overall, SE Afrikelp® demonstrated potential in mitigating the adverse effects of salinity stress on maize plants, warranting its efficacy for agricultural applications.

{"title":"Mitigating salt stress in maize using Ecklonia maxima seaweed extracts","authors":"B.C. Pienaar , B.M. Majeke , M.F. Wittenberg , A.E. Adetunji , L. Nephali , F. Tugizimana , M.S. Rafudeen","doi":"10.1016/j.stress.2025.100828","DOIUrl":"10.1016/j.stress.2025.100828","url":null,"abstract":"<div><div>Salinity stress poses a significant threat to crop productivity, making it crucial to explore strategies that alleviate its adverse effects. This study investigated the impact of Afrikelp®, a commercial seaweed extract (SE) biostimulant, derived from <em>Ecklonia maxima</em> on maize (<em>Zea mays</em> L.) plants subjected to salinity stress. Gas exchange analysis revealed that plants treated with SE Afrikelp® exhibited significantly higher photosynthetic rates under control conditions (45 % respectively) and 200 mM salt stress (95 % respectively) compared to untreated plants. Similarly, plants treated with SE Afrikelp® and 200 mM salt stress had significantly higher internal CO<sub>2</sub> concentrations and transpiration rates compared to untreated salt stressed plants. Morphological analysis demonstrated that SE Afrikelp® enhanced plant growth and reduced electrolyte leakage under both control and salinity stress conditions. Metabolomic profiling revealed significant alterations in primary metabolites, particularly amino acids, sugars, and organic acids. As such, the results reveal that SE Afrikelp® induced a metabolic reprogramming towards stress alleviation and enhanced defences. Overall, SE Afrikelp® demonstrated potential in mitigating the adverse effects of salinity stress on maize plants, warranting its efficacy for agricultural applications.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100828"},"PeriodicalIF":6.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating abiotic stress in citrus: the role of silicon for enhanced productivity and quality

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-02 DOI: 10.1016/j.stress.2025.100837

Jonas Pereira de Souza Junior , Davie M. Kadyampakeni , Muhammad A. Shahid , Renato de Mello Prado , Jose L. Prieto Fajardo

The intensification of global warming has exacerbated abiotic stresses in citrus production, posing significant threats to both fruit yield and quality. Stressors such as drought, extreme temperatures, and salinity disrupt key physiological and biochemical pathways, thus impairing nutrient assimilation, inducing oxidative stress, and affecting fruit development. As climate change continues to amplify these challenges, sustainable mitigation strategies are needed for enhancing citrus resilience. This review explores the multiple effects of abiotic stress on citrus trees and evaluates the role of silicon (Si) as a promising ameliorating agent. Silicon has been increasingly recognized for its capacity to mitigate stress-induced damage through mechanisms such as enhanced photosynthetic efficiency, improved water-use efficiency, upregulated antioxidant defense systems, improved cell wall integrity, and modulation of stress-responsive gene expression. Moreover, Si contributes to maintaining fruit quality by stabilizing biochemical parameters such as sugar concentration, acidity balance, and bioactive compound retention. Despite growing evidence supporting the protective functions of Si, further research is required to optimize its practical application in commercial citrus production. Future studies should focus on elucidating the molecular and physiological pathways underlying Si-mediated stress tolerance and developing targeted Si fertilization suited for varying environmental conditions. Harnessing the potential of Si offers a viable strategy to enhance citrus tree productivity, improve fruit quality, and ensure long-term agricultural sustainability in a changing climate.

{"title":"Mitigating abiotic stress in citrus: the role of silicon for enhanced productivity and quality","authors":"Jonas Pereira de Souza Junior , Davie M. Kadyampakeni , Muhammad A. Shahid , Renato de Mello Prado , Jose L. Prieto Fajardo","doi":"10.1016/j.stress.2025.100837","DOIUrl":"10.1016/j.stress.2025.100837","url":null,"abstract":"<div><div>The intensification of global warming has exacerbated abiotic stresses in citrus production, posing significant threats to both fruit yield and quality. Stressors such as drought, extreme temperatures, and salinity disrupt key physiological and biochemical pathways, thus impairing nutrient assimilation, inducing oxidative stress, and affecting fruit development. As climate change continues to amplify these challenges, sustainable mitigation strategies are needed for enhancing citrus resilience. This review explores the multiple effects of abiotic stress on citrus trees and evaluates the role of silicon (Si) as a promising ameliorating agent. Silicon has been increasingly recognized for its capacity to mitigate stress-induced damage through mechanisms such as enhanced photosynthetic efficiency, improved water-use efficiency, upregulated antioxidant defense systems, improved cell wall integrity, and modulation of stress-responsive gene expression. Moreover, Si contributes to maintaining fruit quality by stabilizing biochemical parameters such as sugar concentration, acidity balance, and bioactive compound retention. Despite growing evidence supporting the protective functions of Si, further research is required to optimize its practical application in commercial citrus production. Future studies should focus on elucidating the molecular and physiological pathways underlying Si-mediated stress tolerance and developing targeted Si fertilization suited for varying environmental conditions. Harnessing the potential of Si offers a viable strategy to enhance citrus tree productivity, improve fruit quality, and ensure long-term agricultural sustainability in a changing climate.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100837"},"PeriodicalIF":6.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency of talcum-biochars in immobilization of heavy metals and promotion of the growth of Brassica chinensis in contaminated agricultural soil 滑石粉生物粘土固定重金属和促进受污染农业土壤中甘蓝菜生长的效率

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-04-02 DOI: 10.1016/j.stress.2025.100836

Hao-Hao Lyu , Kai Cheng , Li-Li He , Sheng-Mao Yang , Yu-Xue Liu , Ling-Cong You , Yu-Ying Wang

Soil contamination with multiple heavy metals poses a significant threat to the global environment. This study aimed to evaluate the ability of a novel talcum-modified biochar as a soil amendment to improve soil properties and remediation performance in agricultural soils contaminated with various heavy metals. Pot experiments were performed in a greenhouse to evaluate the influences of biochar and talcum-modified biochars at 0 %, 0.5 %, 1 %, and 2 % application rates on growth and heavy metal accumulation in Brassica chinensis (B. chinensis). The talcum-biochar composites exhibited superior immobilization efficacy for multiple heavy metals compared to pristine biochar. CaCl₂-heavy metal contents decreased under the treatment with talcum-biochar, in contrast to the control. Sequential extraction procedures revealed that the more accessible forms of heavy metals were converted into less accessible forms. The application of talcum-biochar reduced the Cu, Zn, Cr, and Cd concentrations in B. chinensis, potentially due to a decrease in their bioavailability in the soil. Following the addition of talcum-biochar composites, increases in soil pH, available P and K levels, total N content, and organic matter concentrations were observed. Additionally, a significant enhancement in catalase and urease activities was noted, whereas acid phosphatase activity was inhibited. Therefore, the utilization of talcum-biochar composites as amendments has great potential for enhancing the soil environment and remediating multiple heavy metal-contaminated soils, making it an eco-friendly and cost-effective approach.

{"title":"Efficiency of talcum-biochars in immobilization of heavy metals and promotion of the growth of Brassica chinensis in contaminated agricultural soil","authors":"Hao-Hao Lyu , Kai Cheng , Li-Li He , Sheng-Mao Yang , Yu-Xue Liu , Ling-Cong You , Yu-Ying Wang","doi":"10.1016/j.stress.2025.100836","DOIUrl":"10.1016/j.stress.2025.100836","url":null,"abstract":"<div><div>Soil contamination with multiple heavy metals poses a significant threat to the global environment. This study aimed to evaluate the ability of a novel talcum-modified biochar as a soil amendment to improve soil properties and remediation performance in agricultural soils contaminated with various heavy metals. Pot experiments were performed in a greenhouse to evaluate the influences of biochar and talcum-modified biochars at 0 %, 0.5 %, 1 %, and 2 % application rates on growth and heavy metal accumulation in <em>Brassica chinensis</em> (<em>B. chinensis</em>). The talcum-biochar composites exhibited superior immobilization efficacy for multiple heavy metals compared to pristine biochar. CaCl<sub>2</sub>-heavy metal contents decreased under the treatment with talcum-biochar, in contrast to the control. Sequential extraction procedures revealed that the more accessible forms of heavy metals were converted into less accessible forms. The application of talcum-biochar reduced the Cu, Zn, Cr, and Cd concentrations in <em>B. chinensis</em>, potentially due to a decrease in their bioavailability in the soil. Following the addition of talcum-biochar composites, increases in soil pH, available P and K levels, total N content, and organic matter concentrations were observed. Additionally, a significant enhancement in catalase and urease activities was noted, whereas acid phosphatase activity was inhibited. Therefore, the utilization of talcum-biochar composites as amendments has great potential for enhancing the soil environment and remediating multiple heavy metal-contaminated soils, making it an eco-friendly and cost-effective approach.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100836"},"PeriodicalIF":6.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signaling and scavenging: Unraveling the complex network of antioxidant enzyme regulation in plant cold adaptation

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-03-31 DOI: 10.1016/j.stress.2025.100833

Zhen Ying , Shuangbin Fu , Yanping Yang

Plants have evolved antioxidant defense mechanisms to respond to low - temperature stress. Low temperatures promote ROS accumulation via pathways like photosynthetic chain damage and membrane lipid peroxidation. Excessive ROS harms cellular structures and functions. The plant antioxidant enzyme system, including SOD, CAT, APX, among others, works to scavenge ROS and maintain redox balance. The ICE-CBF-COR signaling pathway, along with transcription factors such as bHLH, WRKY, NAC, and MYB, regulates the expression of antioxidant enzyme genes, thereby enhancing plant cold tolerance. Plant hormones including ABA, BR, JA, and SA also play roles by modulating antioxidant enzyme activity and ROS scavenging capacity. However, many issues remain unresolved, such as the precise regulation of the antioxidant enzyme system, the synergy between different antioxidant enzymes, crosstalk among plant hormones, and the role of non-coding RNAs. Future research should use technologies like yeast one-hybrid, multi-omics, gene editing, high-throughput sequencing, and single-cell sequencing to provide a theoretical basis and regulatory targets for breeding cold-resistant crop varieties.

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引用次数: 0

Enrichment of root-associated bacterial communities by root metabolite profiles influences oilseed rape tolerance to nitrogen deprivation

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-03-31 DOI: 10.1016/j.stress.2025.100827

Lu Yang , Jing Dai , Chiming Gu , Yinshui Li , Wenshi Hu , Yuan Gao , Yingying Zhang , Xing Liao , Lu Qin

Harnessing beneficial plant-microbe interactions in the rhizosphere presents a promising strategy for plants to combat unfavorable environment. However, the mechanisms by which rapeseed (Brassica napus L.) genotypes regulate root-associated microbiota through root metabolites under nitrogen (N) deprivation has not been fully explored. To address this issue, we planted rapeseed genotypes with varying tolerance to N deficiency—G364, which is susceptible, and G294 and ZS11, which exhibit tolerance—under both N-starved (N0) and N-sufficient (N1) conditions in pots. As expected, G364 was the most susceptible to N deficiency, experiencing a 30.8 % reduction in dry biomass when subjected to N deprivation. In contrast, G294 exhibited the greatest tolerance to N-deficiency, with only a 14.1 % decline in biomass due to N deficiency, underscoring its superior N utilization efficiency. The rhizosphere bacterial microbiomes of these genotypes exhibited distinct patterns at the rosette stage. Under N deprivation, the bacterial classes that significantly enriched in the rhizosphere of G294 and ZS11 genotypes were Chloroflexia, Bacilli, TK10, Gammaproteobacteria, and Acidimicrobiia. These microbial enrichments were positively correlated with increased biomass and N uptake in rapeseed. Furthermore, the compositional shifts in the rhizosphere bacterial community were associated with greater intensity of metabolites like flavonoids, amines, terpenoids, steroids, hormones and transmitters etc. Taken together, our study underscores the pivotal role of root metabolites in harnessing the beneficial plant–microbe interactions, thereby potentially improving the N use efficiency of rapeseed. This insight is valuable for manipulating the rhizosphere microbiome for breeding crops aimed at developing varieties with enhanced N efficiency.

{"title":"Enrichment of root-associated bacterial communities by root metabolite profiles influences oilseed rape tolerance to nitrogen deprivation","authors":"Lu Yang , Jing Dai , Chiming Gu , Yinshui Li , Wenshi Hu , Yuan Gao , Yingying Zhang , Xing Liao , Lu Qin","doi":"10.1016/j.stress.2025.100827","DOIUrl":"10.1016/j.stress.2025.100827","url":null,"abstract":"<div><div>Harnessing beneficial plant-microbe interactions in the rhizosphere presents a promising strategy for plants to combat unfavorable environment. However, the mechanisms by which rapeseed (<em>Brassica napus</em> L.) genotypes regulate root-associated microbiota through root metabolites under nitrogen (N) deprivation has not been fully explored. To address this issue, we planted rapeseed genotypes with varying tolerance to N deficiency—G364, which is susceptible, and G294 and ZS11, which exhibit tolerance—under both N-starved (N0) and N-sufficient (N1) conditions in pots. As expected, G364 was the most susceptible to N deficiency, experiencing a 30.8 % reduction in dry biomass when subjected to N deprivation. In contrast, G294 exhibited the greatest tolerance to N-deficiency, with only a 14.1 % decline in biomass due to N deficiency, underscoring its superior N utilization efficiency. The rhizosphere bacterial microbiomes of these genotypes exhibited distinct patterns at the rosette stage. Under N deprivation, the bacterial classes that significantly enriched in the rhizosphere of G294 and ZS11 genotypes were Chloroflexia, Bacilli, TK10, Gammaproteobacteria, and Acidimicrobiia. These microbial enrichments were positively correlated with increased biomass and N uptake in rapeseed. Furthermore, the compositional shifts in the rhizosphere bacterial community were associated with greater intensity of metabolites like flavonoids, amines, terpenoids, steroids, hormones and transmitters etc. Taken together, our study underscores the pivotal role of root metabolites in harnessing the beneficial plant–microbe interactions, thereby potentially improving the N use efficiency of rapeseed. This insight is valuable for manipulating the rhizosphere microbiome for breeding crops aimed at developing varieties with enhanced N efficiency.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100827"},"PeriodicalIF":6.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Growth and Salinity Stress Tolerance in Arabidopsis with Low-Dose Gamma Radiation Priming through a Hormesis Approach

IF 6.8 Q1 PLANT SCIENCES

Plant Stress

Pub Date : 2025-03-31 DOI: 10.1016/j.stress.2025.100834

Daniel Villegas , Constanza Sepúlveda-Hernández , María Jesús Salamé , María Josefina Poupin

Hormesis describes a biphasic response where low-dose exposure triggers positive physiological effects, while higher doses become detrimental. Priming, based on the concept of hormesis, suggests that low doses of a stressor induce beneficial adaptive responses, improving resilience to subsequent, more intense stressors in plants. Ionizing radiation is an interesting method for inducing priming (radio-priming) due to its potential to trigger molecular, biochemical, and physiological responses. Yet, the effects of varying radiation doses, applied at different developmental stages or to distinct plant materials, remain poorly understood, as do the long-term impacts on plant performance. This study evaluated the short- and long-term effects of gamma radiation on Arabidopsis thaliana growth and salinity stress response. Various plant materials were irradiated with increasing gamma doses (5–200 Gray, Gy), and subsequently exposed to salinity stress. Changes in growth, biochemical parameters, gene regulation, and fitness were compared in the different treatments. Low-dose gamma radiation (5–10 Gy) enhanced growth in non-saline and mild salinity conditions, increasing rosette area by 40% in soaked seeds. The 5 Gy treatment also enhanced root growth under severe salinity stress. Conversely, doses exceeding 40 Gy were generally detrimental. Radio-primed plants under salinity stress showed rapid upregulation of LOX2, GLYI7, NHX2, and SOS1. Fitness analysis revealed that the 5 Gy-treated plants produced more seeds per silique under saline conditions. These results confirm that low-dose gamma radiation enhances salinity tolerance in A. thaliana, aligning with the hormesis hypothesis by promoting growth and activating stress-response genes without compromising plant fitness.

{"title":"Enhancing Growth and Salinity Stress Tolerance in Arabidopsis with Low-Dose Gamma Radiation Priming through a Hormesis Approach","authors":"Daniel Villegas , Constanza Sepúlveda-Hernández , María Jesús Salamé , María Josefina Poupin","doi":"10.1016/j.stress.2025.100834","DOIUrl":"10.1016/j.stress.2025.100834","url":null,"abstract":"<div><div>Hormesis describes a biphasic response where low-dose exposure triggers positive physiological effects, while higher doses become detrimental. Priming, based on the concept of hormesis, suggests that low doses of a stressor induce beneficial adaptive responses, improving resilience to subsequent, more intense stressors in plants. Ionizing radiation is an interesting method for inducing priming (radio-priming) due to its potential to trigger molecular, biochemical, and physiological responses. Yet, the effects of varying radiation doses, applied at different developmental stages or to distinct plant materials, remain poorly understood, as do the long-term impacts on plant performance. This study evaluated the short- and long-term effects of gamma radiation on <em>Arabidopsis thaliana</em> growth and salinity stress response. Various plant materials were irradiated with increasing gamma doses (5–200 Gray, Gy), and subsequently exposed to salinity stress. Changes in growth, biochemical parameters, gene regulation, and fitness were compared in the different treatments. Low-dose gamma radiation (5–10 Gy) enhanced growth in non-saline and mild salinity conditions, increasing rosette area by 40% in soaked seeds. The 5 Gy treatment also enhanced root growth under severe salinity stress. Conversely, doses exceeding 40 Gy were generally detrimental. Radio-primed plants under salinity stress showed rapid upregulation of <em>LOX2, GLYI7, NHX2</em>, and <em>SOS1</em>. Fitness analysis revealed that the 5 Gy-treated plants produced more seeds per silique under saline conditions. These results confirm that low-dose gamma radiation enhances salinity tolerance in <em>A. thaliana</em>, aligning with the hormesis hypothesis by promoting growth and activating stress-response genes without compromising plant fitness.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100834"},"PeriodicalIF":6.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0