Zi-Yang He , Hang-Wei Hu , Keren Wu , Li Bi , Shuo Na , Anthony Weatherley , Michael Nash , Ji-Zheng He
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引用次数: 0
Abstract
To improve approaches for studying soil tardigrades, we compared three tardigrade extraction methods, including centrifugal flotation, Baermann funnel, and Whitehead tray, and found that the Whitehead tray method outperformed the two others. We optimised the Whitehead tray extraction condition under various soil pre-treatment processes, sample sizes, light exposure, and extraction time. We found that extending light exposure of non-sieved soils facilitates the extraction of tardigrades. We sequenced the DNA extracted from isolated tardigrade individuals and environmental soil samples, and found that tardigrade DNA extracts yielded a 173-fold increase in the proportion of tardigrade sequences and a 12-fold improvement in the estimation of tardigrade diversity.
为了改进研究土壤中游尸的方法，我们比较了三种游尸提取方法，包括离心浮选法、贝尔曼漏斗法和白石托盘法，发现白石托盘法的效果优于其他两种方法。我们在不同的土壤预处理过程、样本大小、光照和提取时间下对白石托盘提取条件进行了优化。我们发现，延长未筛分土壤的光照时间有利于提取沙丁鱼。我们对从分离的沙丁鱼个体和环境土壤样本中提取的 DNA 进行了测序，发现沙丁鱼 DNA 提取物中沙丁鱼序列的比例增加了 173 倍，沙丁鱼多样性的估计值提高了 12 倍。
期刊介绍：
Soil Biology & Biochemistry publishes original research articles of international significance focusing on biological processes in soil and their applications to soil and environmental quality. Major topics include the ecology and biochemical processes of soil organisms, their effects on the environment, and interactions with plants. The journal also welcomes state-of-the-art reviews and discussions on contemporary research in soil biology and biochemistry.
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