Microbial Community and Functions Depending on Tillage and Straw Returning Management: Consequences for Soil Health and Ecosystem Services

Abstract

Tillage and straw returning are promising practices to improve soil quality, especially because of the unclear controlling effects on microbial communities and functions. A 5-year field experiment in a winter wheat-summer maize cropping system was implemented in the North China Plain to address this research gap. Management practices were deep tillage, rotary tillage, and no-tillage, each with either wheat only or both wheat and maize straw returning. Shotgun metagenomic sequencing was performed to investigate the microbial community, diversity, co-occurrence network, and function in the topsoil (0–20 cm). The associations between soil physiochemical properties and microbial community characteristics were also evaluated. Rotary tillage with only wheat straw returning (RTS) significantly increased Shannon's diversity index by 0.24%–1.71% compared to other treatments. No-tillage with only wheat straw returning (NTS) showed the most stable microbial network with the highest betweenness centrality (199.09), average path distance (2.31), and modularity (0.50). NTS had the highest relative abundance of microbial carbon (C), nitrogen (N) metabolisms, and C fixation pathways. Among deep tillage practices, both wheat and maize straw returning (DTD) were more beneficial to the stability of microbial networks than only wheat straw returning (DTS), yet without improvement in microbial diversity and function. In conclusion, microbial community and function are practical predictors of variations in soil nutrient availability, and pH value in response to different tillage and residue management practices. In particular, our study provides a basis for the development of a sustainable crop residue management system.