{"title":"Aptasensing of rivaroxaban in human plasma using KCC-1-NH-CS2 modified conductive nano-ink: A new biosensor","authors":"Rokhsareh Ebrahimi , Mohammad Hasanzadeh , Nasrin Shadjou , Azadeh Nilghazi","doi":"10.1016/j.microc.2024.111744","DOIUrl":null,"url":null,"abstract":"<div><div>An innovative paper-based electrochemical aptasensor has been developed for the detection of Rivaroxaban (RIV) in human plasma samples. The sensor is fabricated using a novel prob by encapsulating aptamer-aminated on dendritic fibrous nano-silica (KCC-1-NH-CS<sub>2</sub>) particles. The prob is then immobilized on the functionalized silver nano-ink electrode.. The resulting redox reaction on the prob was subsequently evaluated using square wave voltammetry, differential pulse voltammetry, and chronoamperometry. Under optimized condition and using a label-free strategy, the aptasensor exhibited suitable sensitivity and specificity for detecting RIV with a linear range of 10 to 1000 nM and a lower limit of quantification (LLOQ) of 10 nM. The engineered aptasensing platform shows great potential for the fabrication of other cost-effective, sensitive, and portable biosensors for the pharmaceutical and biomedical analysis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111744"},"PeriodicalIF":4.9000,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0026265X24018563","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
An innovative paper-based electrochemical aptasensor has been developed for the detection of Rivaroxaban (RIV) in human plasma samples. The sensor is fabricated using a novel prob by encapsulating aptamer-aminated on dendritic fibrous nano-silica (KCC-1-NH-CS2) particles. The prob is then immobilized on the functionalized silver nano-ink electrode.. The resulting redox reaction on the prob was subsequently evaluated using square wave voltammetry, differential pulse voltammetry, and chronoamperometry. Under optimized condition and using a label-free strategy, the aptasensor exhibited suitable sensitivity and specificity for detecting RIV with a linear range of 10 to 1000 nM and a lower limit of quantification (LLOQ) of 10 nM. The engineered aptasensing platform shows great potential for the fabrication of other cost-effective, sensitive, and portable biosensors for the pharmaceutical and biomedical analysis.
期刊介绍：
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.
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