Molecular and metabolic insights into the mechanism of exogenous methyl jasmonate in enhancing the postharvest resistance of kiwifruit to Botrytis cinerea

IF 6.4 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2024-09-25 DOI:10.1016/j.postharvbio.2024.113226

Jiaqi Yang , Yijia Ma , Tianjing Zeng, Zhexin Li, Yuan Sui, Wenlin Zhang, Hongpan Zhong, Xu Wang

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Abstract

Gray mold, caused by Botrytis cinerea infection, significantly impacts postharvest kiwifruit, leading to spoilage and food safety issues. Meanwhile, methyl jasmonate (MeJA) induces defense responses in plants. This study aimed to identify the optimal MeJA concentration to induce disease resistance in kiwifruits. Notably, various plant defense enzymes were detected in MeJA-treated kiwifruit. Moreover, 0.01 mol/L MeJA was found to enhance B. cinerea resistance in kiwifruit by increasing antioxidant enzyme activity. Widely targeted metabolomics analysis revealed 62–64 differentially expressed metabolites (DEMs) between the different treatment groups. Additionally, RNA-seq analysis revealed 930–2900 differentially expressed genes (DEGs), between these groups. Subsequently, combined DEG and DEM analysis highlighted phenylpropanoid biosynthesis and plant hormone signaling as key transduction pathway associated with MeJA-induced resistance. Overall, these findings identified the mechanism of MeJA-induced resistance in kiwifruit, providing a theoretical basis for the safe and effective control of postharvest diseases in kiwifruit.

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外源茉莉酸甲酯增强猕猴桃采后对灰葡萄孢菌抗性的分子和代谢机理探究

由灰霉病菌（Botrytis cinerea）感染引起的灰霉病严重影响采后猕猴桃，导致变质和食品安全问题。同时，茉莉酸甲酯（MeJA）可诱导植物产生防御反应。本研究旨在确定诱导猕猴桃抗病的最佳 MeJA 浓度。值得注意的是，在经 MeJA 处理的猕猴桃中检测到了多种植物防御酶。此外，研究还发现 0.01 mol/L MeJA 能通过提高抗氧化酶的活性来增强猕猴桃的抗病性。广泛的靶向代谢组学分析显示，不同处理组之间存在 62-64 个差异表达代谢物（DEMs）。此外，RNA-seq 分析还发现这些组间存在 930-2900 个差异表达基因（DEG）。随后，结合 DEG 和 DEM 分析，发现苯丙酮生物合成和植物激素信号转导是与 MeJA 诱导的抗性相关的关键传导途径。总之，这些发现确定了 MeJA 诱导猕猴桃抗性的机制，为安全有效地控制猕猴桃采后病害提供了理论依据。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.