A novel ratiometric colorimetric sensor for detecting hypochlorite and ascorbic acid based on cascade reaction

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2024-09-23 DOI:10.1016/j.ab.2024.115678

Miaowen Sun, Yanzhu Liu, Lingge Shi, Haomiao Dou, Feiyan Ma, Guangda Xu, Longshan Zhao

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Abstract

Hypochlorite and ascorbic acid (AA), play an indispensable role in numerous physiological activities. Herein, a ratiometric colorimetric sensing strategy for the determination of hypochlorite and AA was developed via the catalytic oxidation and reduction of 3,3′,5,5′-tetramethylbenzidine (TMB). Interestingly, in the presence of Fe₃O₄-MOF-5(Fe) and hypochlorite, TMB complexes in acidic environments were oxidized to blue oxidized TMB and further diazotized to produce yellow-green diazotized TMB, resulting in the hypochlorite concentration-dependent ratiometric variation for the absorbance at 652 and 450 nm (A₄₅₀/A₆₅₂). Moreover, the diazotized TMB was restored to colorless TMB due to the reducibility of AA, and the detection limit of hypochlorite and AA were 0.027 and 0.677 μM, respectively. The ratiometric colorimetric sensing platform offered higher sensitivity and better selectivity because of the specific hypochlorite-induced reaction and the excellent peroxidase-like activity of Fe₃O₄-MOF-5(Fe). The proposed novel strategy provided the guidance to develop sensors for successive detection of hypochlorite and AA in complicated samples.

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基于级联反应检测次氯酸盐和抗坏血酸的新型比率比色传感器

次氯酸盐和抗坏血酸（AA）在多种生理活动中发挥着不可或缺的作用。本文通过催化 3,3′,5,5′-四甲基联苯胺（TMB）的氧化和还原，开发了一种测定次氯酸盐和抗坏血酸的比色法传感策略。有趣的是，在 Fe3O4-MOF-5(Fe)和次氯酸盐存在的情况下，TMB 复合物在酸性环境中被氧化成蓝色氧化 TMB，并进一步重氮化生成黄绿色重氮化 TMB，从而导致 652 纳米和 450 纳米吸光度（A450/A652）随次氯酸盐浓度的比率变化。此外，由于 AA 的还原性，重氮化 TMB 恢复为无色 TMB，次氯酸盐和 AA 的检测限分别为 0.027 和 0.677 μM。由于次氯酸盐诱导反应的特异性和 Fe3O4-MOF-5(Fe) 卓越的过氧化物酶样活性，比色法传感平台具有更高的灵敏度和更好的选择性。所提出的新策略为开发用于连续检测复杂样品中次氯酸盐和 AA 的传感器提供了指导。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.