Distribution characteristics of Purkinje fibres in the canine left ventricle

Yunhao Li, Daoyang Zhang, Yunfan Meng, Jie Zhang, Qi Zhang, Ping Zhang, Yujie Zhang, Mingyu Sun, Zulu Wang, Ming Liang
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Abstract

Purkinje-related ventricular arrhythmias have been increasingly reported, and with the development of catheter ablation techniques, intervention for Purkinje-related arrhythmias has been shown to be effective. The characteristics of Purkinje fibres orientation in the 12 canine left ventricles were observed at a gross level by staining the endocardium with Lugol's solution. Purkinje fibres were observed microscopically by HE, Masson's, PAS glycogen, and Cx40 immunohistochemical staining. Staining was successful, and the transverse orientation characteristics of Purkinje fibres were observed by Lugol's staining, and the longitudinal distribution was observed microscopically. The distribution of Purkinje fibres in the canine left ventricle is ‘graded’, ‘layered’, and ‘networked’, which can guide catheter ablation of Purkinje-related ventricular arrhythmia.

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犬左心室浦肯野纤维的分布特征
与浦肯野相关的室性心律失常的报道越来越多,随着导管消融技术的发展,对浦肯野相关心律失常的干预已被证明是有效的。通过用 Lugol's 溶液对心内膜进行染色,在大体上观察了 12 只犬左心室中 Purkinje 纤维的取向特征。显微镜下通过 HE、Masson's、PAS 糖原和 Cx40 免疫组化染色观察浦肯野纤维。染色成功,通过卢戈氏染色观察到了浦肯野纤维的横向定向特征,并在显微镜下观察到了纵向分布。犬左心室浦肯野纤维的分布具有 "分级"、"分层 "和 "网络化 "的特点,可为浦肯野相关室性心律失常的导管消融提供指导。
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11.50
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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