Vascular network-mediated systemic spread of Pseudomonas syringae pv. actinidiae causes the bacterial canker of kiwifruit

IF 5.7 1区 农林科学 Q1 HORTICULTURE Horticultural Plant Journal Pub Date : 2024-09-14 DOI:10.1016/j.hpj.2024.05.007
Runze Tian, Yujie Tian, Qianqian Dang, Hongchang Zhang, Lili Huang
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Abstract

Pseudomonas syringae pv. actinidiae (Psa) causes destructive kiwifruit bacterial canker by invading vascular tissues across multiple plant organs. However, the cellular mechanism underlying its systemic transmission and cell-to-cell movement within these specialized vascular conduits remains unclear. In this study, a Psa-GFP strain and various microscopic techniques were used to investigate the interaction between kiwifruit and Psa. Our results reveal that Psa strategically exploits host vascular conduits for systemic movement, with the xylem vessel being the predominant avenue. In the phloem, Psa exhibits adaptive alteration in bacterial shape to traverse sieve pores, facilitating its systemic spread along sieve tubes and inducing phloem necrosis. Within the xylem, Psa breaches pit membranes to migrate between adjacent vessels. Furthermore, phloem fibers act as an initial barrier at the early stages of infection, delaying Psa's entry into vascular tissues during its journey to the xylem. Additionally, at the junctions of stem–stem or stem-leaf, branch trace or leaf trace mediates the bacterial organ-to-organ translocation, thus facilitating the systemic progression of disease. In conclusion, our findings shed light on the cellular mechanism employed by Psa to exploit the woody plant's vascular network for infection, thereby enhancing a better understanding of the biology of this poorly defined bacterium. These insights carry implications for the pathogenesis of Psa and other vascular pathogens, offering theoretical guidance for effective control strategies.
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由维管束网络介导的猕猴桃细菌性腐烂病(Pseudomonas syringae pv. actinidiae)的系统性传播
Pseudomonas syringae pv. actinidiae(Psa)通过侵入多个植物器官的维管组织,引起破坏性的猕猴桃细菌性腐烂病。然而,其在这些专门的维管导管中的系统传播和细胞间移动的细胞机制仍不清楚。本研究利用 Psa-GFP 菌株和各种显微镜技术研究了猕猴桃与 Psa 之间的相互作用。 我们的研究结果表明,Psa 有策略地利用寄主的维管导管进行系统运动,其中木质部血管是最主要的途径。在韧皮部,Psa 会适应性地改变细菌形状以穿过筛孔,从而促进其沿着筛管进行系统性传播并诱导韧皮部坏死。在木质部中,Psa 冲破坑膜,在相邻血管之间迁移。此外,在感染的早期阶段,韧皮部纤维起着初始屏障的作用,在 Psa 进入木质部的过程中,可延缓其进入血管组织。此外,在茎-茎或茎-叶的交界处,枝痕或叶痕介导了细菌在器官间的转移,从而促进了病害的系统进展。总之,我们的发现揭示了 Psa 利用木本植物的维管网络进行感染的细胞机制,从而加深了对这种定义不清的细菌的生物学特性的了解。这些发现对 Psa 及其他维管束病原体的致病机理具有重要意义,为有效的控制策略提供了理论指导。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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