Ultrasound-assisted extraction of polyphenols from Phyllanthi Fructus: Comprehensive insights from extraction optimization and antioxidant activity

IF 8.7 1区化学 Q1 ACOUSTICS Ultrasonics Sonochemistry Pub Date : 2024-09-25 DOI:10.1016/j.ultsonch.2024.107083

Haixia Che , Rui Zhang , Xianyao Wang , Hong Yu , Xuepeng Shi , Jie Yi , Jiawen Li , Qi Qi , Ruizhi Dong , Qian Li

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Abstract

Phyllanthi Fructus (PF) is a valuable botanical resource with a long history of traditional use, known for potent antioxidant and anti-inflammatory effects attributed to its rich contents of bioactive compounds, particularly polyphenols. However, current extraction techniques limit the utilization of polyphenols from PF. This study aimed to achieve the maximum polyphenol yield and improve the antioxidant activity of PF extracts to promise PF’s prospects for modern healthcare.

Firstly, ultrasonic-assisted extraction (UAE) was employed to extract the polyphenols in PF and a combination of Plackett-Burman designs (PBD) and response surface methodology (RSM) was applied to optimize UAE’s conditions. Next, cellular superoxide dismutase (SOD) and malondialdehyde (MDA) were used to assess the antioxidant activity of extracted polyphenols. Ultra-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF MS) was utilized to characterize polyphenol components in the PF extracts. Finally, network pharmacology and molecular docking analysis were performed to screen the potential target proteins of polyphenols from PF.

As a result, the optimized polyphenol yield was 213.49 mg/g, and the antioxidant activities, measured by ability of DPPH scavenging, ABTS+ scavenging, and FRAP were 76.95 %, 2.24 mmol/g, 2.34 mmol/g, respectively. PF extracts also showed good antioxidant capabilities at cellular level. 26 polyphenol components were identified in the PF extracts. Among these, ellagic acid, myricetin, and eriodictyol may exert antioxidant effects by interacting with AKT serine/threonine kinase 1 (AKT1).

In conclusion, our study provides valuable insights into the optimizing PF extraction and underscores its potential applications in enhancing natural polyphenols extraction using UAE with a combination of PBD and RSM. These findings offer a promising avenue for the development and utilization of PF, and could serve as a reference for similar extraction processes in the future.

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超声波辅助萃取叶黄素中的多酚：从萃取优化和抗氧化活性中获得全面启示

叶下珠（Phyllanthi Fructus，PF）是一种宝贵的植物资源，具有悠久的传统使用历史，因其富含生物活性化合物（尤其是多酚）而具有强大的抗氧化和消炎作用。然而，目前的提取技术限制了对 PF 中多酚的利用。首先，采用超声波辅助萃取（UAE）来提取 PF 中的多酚，并结合普拉克特-伯曼设计（PBD）和响应面方法（RSM）来优化 UAE 的条件。然后，使用细胞超氧化物歧化酶（SOD）和丙二醛（MDA）来评估提取多酚的抗氧化活性。超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF MS）用于鉴定 PF 提取物中的多酚成分。结果表明，优化后的多酚产量为 213.49 mg/g，DPPH 清除能力、ABTS+ 清除能力和 FRAP 抗氧化活性分别为 76.95 %、2.24 mmol/g、2.34 mmol/g。PF 提取物在细胞水平上也表现出良好的抗氧化能力。在 PF 提取物中发现了 26 种多酚成分。总之，我们的研究为优化 PF 提取提供了有价值的见解，并强调了其在使用结合 PBD 和 RSM 的 UAE 提高天然多酚提取方面的潜在应用。这些研究结果为开发和利用 PF 提供了一条前景广阔的途径，并可作为未来类似提取工艺的参考。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.