The protective effect of two highly branched polysaccharides from corn silk fermented by Lactobacillus plantarum against acute liver injury

Abstract

To valorize the natural resource of corn silk as hepatoprotective food supplement, two fractions of polysaccharides (F-CSP-W/S) have been separated and purified from corn silk fermented by Lactobacillus plantarum. F-CSP-W was mainly composed of Glc, Gal, Ara, Xyl, Man (66.62: 11.83: 11.59: 7.89:1.48), traces of GlcN and GlcA; whereas F-CSP-S was mainly composed of Glc, Xyl, Gal, Ara, GlcA, GalA, Rha, Man (39.80: 18.26: 17.04: 11.87: 3.33: 3.18: 3.04: 2.77) and traces of GlcN. The detailed chemical structure analysis showed F-CSP-W/S were highly branched polysaccharides, which possessed the backbone of α-1 → 4-Glcp branched at α-1 → 3-Galp, with the solvated domains of arabinoxylan mainly consisted of β-1 → 4-Xyl, α-Ara and 4-O-Me-α-t-Glcp UA as side chains. F-CSP-S had a higher branch degree with pectin like side chain that contained partial methyl-esterificated α-1 → 4-GalUA and 1 → 2-Rhap covalently bound to arabinoxylan. Meanwhile, F-CSP-S displayed potent anti-oxidant activity in vitro. Both F-CSP-W and F-CSP-S ameliorated CCl₄-induced mouse acute liver injury through directly hepatoprotective effect as verified by the organ index, liver function, serum enzymatic activities and histological changes of liver.