Xiaoxin Zhao, Shuoqi Huang, Qi Yao, Rui He, Hao Wang, Zhaodan Xu, Wang Xing, Dali Liu
{"title":"ABA-regulated MAPK signaling pathway promotes hormesis in sugar beet under cadmium exposure","authors":"Xiaoxin Zhao, Shuoqi Huang, Qi Yao, Rui He, Hao Wang, Zhaodan Xu, Wang Xing, Dali Liu","doi":"10.1016/j.jhazmat.2024.135968","DOIUrl":null,"url":null,"abstract":"Sugar beet (<em>Beta vulgaris</em> L.) shows potential as an energy crop for cadmium (Cd) phytoremediation. To elucidate its <em>in vivo</em> response strategy to Cd exposure, seedlings were treated with 1, 3, and 5<!-- --> <!-- -->mmol/L CdCl<sub>2</sub> (Cd-1, Cd-3, and Cd-5) for 6<!-- --> <!-- -->h, using 0<!-- --> <!-- -->mmol/L CdCl<sub>2</sub> (Cd-0) as the control. The results showed that Cd-3 promoted a unique “hormesis” effect, leading to enhanced growth performance, increased levels of chlorophyll, soluble protein, and SOD activity, and reduced MDA content in sugar beet, compared to Cd-1, Cd-5, and even Cd-0. GO and KEGG enrichments and PPI network of transcriptomic analysis revealed that the differentially expressed genes (DEGs) were primarily involved in lipid metabolism, cellular protein catabolism, and photosynthesis. Notably, the MAPK signaling pathway was significantly enriched only under Cd-3, with the up-regulation of ABA-related core gene <em>BvPYL9</em> and an increase in ABA content after 6<!-- --> <!-- -->h of Cd exposure. Furthermore, overexpression of <em>BvPYL9</em> in <em>Arabidopsis thaliana</em> (OE-1 and OE-2) resulted in enhanced growth (fresh weight, dry weight, root length), as well as higher ABA and soluble protein contents. Cd-induced transcriptional responses of <em>BvPYL9</em> were also evident in OE-1 and OE-2, especially at 10 µmol/L, indicated by qRT-PCR. These findings suggest that ABA-mediated MAPK signaling pathway is activated in response to Cd toxicity, with <em>BvPYL9</em> being a key factor in the cascade effects for the Cd-induced hormesis in sugar beet.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2024.135968","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Sugar beet (Beta vulgaris L.) shows potential as an energy crop for cadmium (Cd) phytoremediation. To elucidate its in vivo response strategy to Cd exposure, seedlings were treated with 1, 3, and 5 mmol/L CdCl2 (Cd-1, Cd-3, and Cd-5) for 6 h, using 0 mmol/L CdCl2 (Cd-0) as the control. The results showed that Cd-3 promoted a unique “hormesis” effect, leading to enhanced growth performance, increased levels of chlorophyll, soluble protein, and SOD activity, and reduced MDA content in sugar beet, compared to Cd-1, Cd-5, and even Cd-0. GO and KEGG enrichments and PPI network of transcriptomic analysis revealed that the differentially expressed genes (DEGs) were primarily involved in lipid metabolism, cellular protein catabolism, and photosynthesis. Notably, the MAPK signaling pathway was significantly enriched only under Cd-3, with the up-regulation of ABA-related core gene BvPYL9 and an increase in ABA content after 6 h of Cd exposure. Furthermore, overexpression of BvPYL9 in Arabidopsis thaliana (OE-1 and OE-2) resulted in enhanced growth (fresh weight, dry weight, root length), as well as higher ABA and soluble protein contents. Cd-induced transcriptional responses of BvPYL9 were also evident in OE-1 and OE-2, especially at 10 µmol/L, indicated by qRT-PCR. These findings suggest that ABA-mediated MAPK signaling pathway is activated in response to Cd toxicity, with BvPYL9 being a key factor in the cascade effects for the Cd-induced hormesis in sugar beet.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.