{"title":"Revealing the bioavailability and phytotoxicity of different particle size microplastics on diethyl phthalate (DEP) in rye (Secale cereale L.)","authors":"Jinke Hu, Guozhang Bao, Xinyu Pan, Huixin Wang, Ningning Xing","doi":"10.1016/j.jhazmat.2024.135979","DOIUrl":null,"url":null,"abstract":"Understanding how widely distributed microplastics (MPs) and diethyl phthalate (DEP) interact with crops remains limited, despite their significant implications for human exposure. We used physiology, transcriptomics, adsorption kinetics, and computational chemistry to assess rye’s molecular response to two sizes of MPs (200<!-- --> <!-- -->nm and 5 μm) and DEP, both individually and in combination. Findings systematically highlight potential ecological risks from MPs and DEP, with ecotoxicity ranking as follows: CK (Control Check) < LMPs < SMPs < DEP < LMPs+DEP < SMPs+DEP. Fluorescence and scanning electron microscopy revealed SMP’s translocation ability in rye and its potential to disrupt leaf cells. DEP increased the negative charge on MPs, which enhanced their uptake by rye. DEP adsorption by MPs in hydroponics reduced DEP bioavailability in rye (18.17-46.91%). Molecular docking studies showed DEP interacted with chlorophyll, superoxide dismutase, and glutathione S-transferases proteins’ active sites. Transcriptomic analysis identified significant up-regulation of genes linked to mitogen-activated protein kinase signaling, phytohormones, and antioxidant systems in rye exposed to MPs and DEP, correlating with physiological changes. These findings deepen the understanding of how MPs can accumulate and translocate within rye, and their adsorption to DEP raises crop safety issues of greater environmental risk.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2024.135979","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Understanding how widely distributed microplastics (MPs) and diethyl phthalate (DEP) interact with crops remains limited, despite their significant implications for human exposure. We used physiology, transcriptomics, adsorption kinetics, and computational chemistry to assess rye’s molecular response to two sizes of MPs (200 nm and 5 μm) and DEP, both individually and in combination. Findings systematically highlight potential ecological risks from MPs and DEP, with ecotoxicity ranking as follows: CK (Control Check) < LMPs < SMPs < DEP < LMPs+DEP < SMPs+DEP. Fluorescence and scanning electron microscopy revealed SMP’s translocation ability in rye and its potential to disrupt leaf cells. DEP increased the negative charge on MPs, which enhanced their uptake by rye. DEP adsorption by MPs in hydroponics reduced DEP bioavailability in rye (18.17-46.91%). Molecular docking studies showed DEP interacted with chlorophyll, superoxide dismutase, and glutathione S-transferases proteins’ active sites. Transcriptomic analysis identified significant up-regulation of genes linked to mitogen-activated protein kinase signaling, phytohormones, and antioxidant systems in rye exposed to MPs and DEP, correlating with physiological changes. These findings deepen the understanding of how MPs can accumulate and translocate within rye, and their adsorption to DEP raises crop safety issues of greater environmental risk.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.