High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation.

IF 4.5 2区 医学 Q2 IMMUNOLOGY Journal of Microbiology Immunology and Infection Pub Date : 2024-09-21 DOI:10.1016/j.jmii.2024.09.005
Yu-Tzu Lin, Ngoc-Niem Bui, Yu-Syuan Cheng, Cheng-Wen Lin, Chun-Li Lee, Tai-Fen Lee, Po-Ren Hsueh
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Abstract

Background: α-Hemolysin, encoded by hla, is a major virulence factor of Staphylococcus aureus. Sequence type (ST) 45 is a globally spread clone with increasing clinical prevalence in Taiwan. Our previous study showed that among the CC45 isolates, the spa type t1081 isolates presented greater hemolytic activity.

Materials and methods: The hemolytic activity of 67 CC45 isolates (44 t1081 and 23 non-t1081) from clinical blood cultures was assessed using rabbit red blood cells. The sequences of hla and its upstream regulatory regions and RNAIII were compared between the two groups. The expression of hla and its regulators RNAIII, mgrA, and saeR was analyzed via qRT‒PCR, while Hla protein levels were measured via Western blotting.

Results: Compared with non-t1081 isolates, t1081 isolates presented increased hemolytic activity. No significant differences in hla sequences, upstream regulatory regions, or gene expression levels were detected between the two groups. The expression of the transcriptional regulators mgrA and saeR was also similar between the two groups. Western blotting revealed increased Hla protein in the t1081 isolates. However, neither the sequence or expression of RNAIII, a regulator of hla at both the transcriptional and posttranscriptional levels, differed between the groups.

Conclusion: Our study revealed that, compared with other CC45 isolates, the t1081/ST45 isolates presented greater hemolytic activity. This heightened activity was due mainly to increased Hla protein levels. Moreover, the higher translation levels may be independent of the known regulator RNAIII, indicating a potential RNAIII-independent mechanism for Hla regulation.

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金黄色葡萄球菌 t1081/ST45 的高溶血活性是由于 hla 蛋白生成增加和潜在的 RNAIII 非依赖性调控所致。
背景:由 hla 编码的 α 溶血素是金黄色葡萄球菌的主要毒力因子。序列类型(ST)45 是一种全球传播的克隆,在台湾的临床流行率越来越高。我们之前的研究表明,在 CC45 分离物中,t1081 型 spa 分离物具有更强的溶血活性:用兔红细胞评估了临床血液培养物中 67 个 CC45 分离物(44 个 t1081 型和 23 个非 t1081 型)的溶血活性。比较了两组细胞中 hla 及其上游调控区和 RNAIII 的序列。通过 qRT-PCR 分析了 hla 及其调控因子 RNAIII、mgrA 和 saeR 的表达,并通过 Western 印迹检测了 Hla 蛋白水平:结果:与非 t1081 分离物相比,t1081 分离物的溶血活性增强。两组分离物的 hla 序列、上游调控区和基因表达水平均无明显差异。两组之间转录调控因子 mgrA 和 saeR 的表达也相似。Western 印迹显示,t1081 分离物中的 Hla 蛋白增加了。然而,RNAIII(hla 在转录和转录后水平的调控因子)的序列和表达在两组间均无差异:我们的研究表明,与其他 CC45 分离物相比,t1081/ST45 分离物具有更强的溶血活性。这种活性的增强主要是由于 Hla 蛋白水平的提高。此外,较高的翻译水平可能与已知的调节因子 RNAIII 无关,这表明 Hla 的调节机制可能与 RNAIII 无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Microbiology Immunology and Infection
Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES
CiteScore
15.90
自引率
5.40%
发文量
159
审稿时长
67 days
期刊介绍: Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.
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