Unusual photodynamic characteristics of the light-oxygen-voltage domain of phototropin linked to terrestrial adaptation of Klebsormidium nitens.

Sunita Sharma, Avinash Kumar Gautam, Rajani Singh, Samudrala Gourinath, Suneel Kateriya
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Abstract

Phototropin (Phot), a blue light-sensing LOV domain protein, mediates blue light responses and is evolutionarily conserved across the green lineage. Klebsormidium nitens, a green terrestrial alga, presents a valuable opportunity to study adaptive responses from aquatic to land habitat transitions. We determined the crystal structure of Klebsormidium nitens Phot LOV1 domain (KnLOV1) in the dark and engineered different mutations (R60K, Q122N, and D33N) to modulate the lifetime of the photorecovery cycle. We observed unusual, slow recovery kinetics in the wild-type KnLOV1 domain (τ = 41 ± 3 min) compared to different mutants (R60K: τ = 2.0 ± 0.1 min, Q122N: τ = 1.7 ± 0.1 min, D33N: τ = 9.6 ± 0.1 min). Crystal structures of wild-type KnLOV1 and mutants revealed subtle but critical changes near the protein chromophore that is responsible for modulating protein dark recovery time. Our findings shed light on the unique structural and biochemical characteristics of the newly studied KnLOV1 and its evolutionary importance for phototropin-mediated physiology.

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与硝酸克雷伯虫陆地适应有关的光蛋白光氧电压结构域的非同寻常的光动力特性。
光感蛋白(Phototropin,Phot)是一种蓝光传感 LOV 结构域蛋白,介导蓝光反应,在整个绿色藻系中具有进化保守性。硝化克雷伯藻是一种绿色陆生藻类,它为研究从水生到陆生生境转换的适应性反应提供了一个宝贵的机会。我们测定了硝虫光合 LOV1 结构域(KnLOV1)在黑暗中的晶体结构,并设计了不同的突变(R60K、Q122N 和 D33N)来调节光复周期的寿命。与不同的突变体(R60K:τ = 2.0 ± 0.1 分钟;Q122N:τ = 1.7 ± 0.1 分钟;D33N:τ = 9.6 ± 0.1 分钟)相比,我们观察到野生型 KnLOV1 结构域的恢复动力学异常缓慢(τ = 41 ± 3 分钟)。野生型 KnLOV1 和突变体的晶体结构揭示了负责调节蛋白质暗恢复时间的蛋白质发色团附近微妙但关键的变化。我们的发现揭示了新研究的 KnLOV1 的独特结构和生化特征,以及它对光促蛋白介导的生理学进化的重要性。
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