Madison B Adolph, Garrett M Warren, Frank B Couch, Briana H Greer, Brandt F Eichman, David Cortez
{"title":"WITHDRAWN: Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF.","authors":"Madison B Adolph, Garrett M Warren, Frank B Couch, Briana H Greer, Brandt F Eichman, David Cortez","doi":"10.1101/2024.09.17.613558","DOIUrl":null,"url":null,"abstract":"<p><p>The authors have withdrawn this manuscript because they identified problems with how some figure panels were processed. Those experiments will be repeated before deposition of a new manuscript. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding authors.</p>","PeriodicalId":519960,"journal":{"name":"bioRxiv : the preprint server for biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11429910/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"bioRxiv : the preprint server for biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/2024.09.17.613558","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The authors have withdrawn this manuscript because they identified problems with how some figure panels were processed. Those experiments will be repeated before deposition of a new manuscript. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding authors.
在 DNA 复制过程中，复制体会遇到各种障碍，包括 DNA 损伤、转录-复制冲突以及其他复制压力源。必须有效克服这些障碍，才能完成 DNA 合成，并将基因组的不稳定性降至最低。耐受复制压力的途径之一是复制叉逆转，在这一过程中，亲代模板 DNA 链重新接合，并形成新生-新生 DNA 双链。有几种酶能促进复制叉逆转，包括依赖 ATP 的转位酶 SMARCAL1、ZRANB3 和 HLTF。这些酶如何在含有分叉分离病变的 DNA 上进行转位尚不清楚。在这里，我们研究了 SMARCAL1、ZRANB3 和 HLTF 对前导或滞后模板链上各种病变的耐受能力。我们证明，SMARCAL1 和 ZRANB3 会选择性地抑制前导模板链上的病变，而 HLTF 对任一模板链上的大块病变都不敏感。这些结果表明，在分叉逆转过程中，SMARCAL1 和 ZRANB3 与前导链接触，因此对该链上大块病变的抑制更敏感。相比之下，HLTF DNA 易位对 DNA 损伤不敏感。叉形反转酶之间的这些生化差异使人们对它们的DNA重塑机制有了更深入的了解，并表明它们可能在特异性病变环境中发挥作用。
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
