From ice to neurons: investigating the neuroprotective effects of Antarctic microalgae Chlorella variabilis and Chlorella pyrenoidosa extracts.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-26 DOI:10.1007/s13205-024-04094-4
Benan İnan, Betül Mutlu, Rabia Çakır, Didem Balkanlı
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Abstract

In this study, it was aimed to examine the neuroprotective effects of ethanolic extracts of Chlorella variabilis YTU.ANTARCTIC.001 and Chlorella pyrenoidosa OZCIMEN.001 microalgae that were isolated from Antarctica in a H2O2-induced oxidative stress model using SH-SY5Y cell line. In this context, first, Antarctic microalgae were cultivated and characterized. It was determined that C. pyrenoidosa and C. variabilis had specific growth rates of 0.093 and 0.097 day-1, respectively, and doubled their cell concentration in 7 days. With the antioxidant and phenolic content analysis, it was found that 1 mg/mL C. pyrenoidosa and C. variabilis ethanolic extracts had 33-37% radical scavenging activity and 102-107 mg GAE/mg extract phenolic content, respectively. Then, the cytotoxic effects of the microalgae extracts on SH-SY5Y cells were assessed across a concentration range of 6.25-125 µg/mL. The results indicated a concentration-dependent effect on cell viability, with no observed cytotoxicity within the tested range. Notably, the highest neuroprotective activity was recorded with C. variabilis extract at a concentration of 75 µg/mL, which maintained cell viability at 73.7% ± 0.3. These findings showed the significant neuroprotective potential of C. pyrenoidosa and C. variabilis ethanolic extracts, attributed to their substantial antioxidant properties and non-cytotoxic nature at effective concentrations. The promising neuroprotective efficacy of these extracts highlights their potential for therapeutic applications in neurodegenerative disease prevention and treatment.

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从冰到神经元:研究南极微藻类小球藻和小球藻萃取物的神经保护作用。
本研究旨在利用 SH-SY5Y 细胞系,在 H2O2 诱导的氧化应激模型中研究从南极洲分离的变种小球藻 YTU.ANTARCTIC.001 和拟小球藻 OZCIMEN.001 的乙醇提取物对神经的保护作用。在此背景下,首先对南极微藻进行了培养和特征描述。结果表明,C. pyrenoidosa 和 C. variabilis 的特定生长率分别为 0.093 和 0.097 day-1,并在 7 天内使其细胞浓度增加一倍。通过对抗氧化剂和酚含量的分析发现,1 mg/mL的焦褐藻和变种褐藻乙醇提取物分别具有33-37%的自由基清除活性和102-107 mg GAE/mg提取物酚含量。然后,在 6.25-125 µg/mL 的浓度范围内评估了微藻提取物对 SH-SY5Y 细胞的细胞毒性作用。结果表明,微藻提取物对细胞活力的影响与浓度有关,在测试范围内未观察到细胞毒性。值得注意的是,变异草提取物的神经保护活性最高,浓度为 75 µg/mL 时,细胞存活率保持在 73.7% ± 0.3。这些研究结果表明,C. pyrenoidosa和C. variabilis乙醇提取物具有明显的神经保护潜力,这要归功于它们在有效浓度下的抗氧化性和无细胞毒性。这些提取物具有良好的神经保护功效,突出了它们在预防和治疗神经退行性疾病方面的治疗应用潜力。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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