首页 > 最新文献

3 Biotech最新文献

英文 中文
Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications. 生物表面活性剂的前沿视角:对抗菌和生物医学应用的影响。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1007/s13205-024-04146-9
Kashish Gupta, Varun Kumar Sharma

Biosurfactants, naturally produced by plants and microorganisms, closely mimic synthetic surfactants in physiochemical properties, making them valuable alternatives in various applications. They serve as antimicrobial agents and play a crucial role in immune regulations. These compounds find wide use in industries like food processing, biodegradation, pharmaceuticals, and naturally present in the skin, brain, lungs, and gut, maintaining membrane permeability for organ health. This review outlines the basic characteristics and classes of biosurfactants (glycolipids, lipopeptides, phospholipids, and glycoproteins) and explores their biomedical importance, emphasizing their anti-adhesive, antimicrobial, and immune-modulating properties. This review aimed to provide outline the fundamental characteristics of biosurfactants and deliver a brief overview of their different classes, including glycolipids, lipopeptides, phospholipids, and glycoproteins. Furthermore, this review also explore their biomedical significance, highlighting their anti-adhesive, antimicrobial, and immune-modulating properties.

由植物和微生物天然产生的生物表面活性剂在理化性质上与合成表面活性剂非常相似,因此在各种应用中都是有价值的替代品。它们是抗菌剂,在免疫调节中发挥着重要作用。这些化合物广泛应用于食品加工、生物降解、制药等行业,并天然存在于皮肤、大脑、肺部和肠道中,保持膜的渗透性,促进器官健康。这篇综述概述了生物表面活性剂(糖脂、脂肽、磷脂和糖蛋白)的基本特征和类别,并探讨了它们在生物医学方面的重要性,强调了它们的抗粘附、抗菌和免疫调节特性。本综述旨在概述生物表面活性剂的基本特征,并简要介绍其不同类别,包括糖脂、脂肽、磷脂和糖蛋白。此外,这篇综述还探讨了生物表面活性剂的生物医学意义,强调了它们的抗粘性、抗菌性和免疫调节特性。
{"title":"Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications.","authors":"Kashish Gupta, Varun Kumar Sharma","doi":"10.1007/s13205-024-04146-9","DOIUrl":"10.1007/s13205-024-04146-9","url":null,"abstract":"<p><p>Biosurfactants, naturally produced by plants and microorganisms, closely mimic synthetic surfactants in physiochemical properties, making them valuable alternatives in various applications. They serve as antimicrobial agents and play a crucial role in immune regulations. These compounds find wide use in industries like food processing, biodegradation, pharmaceuticals, and naturally present in the skin, brain, lungs, and gut, maintaining membrane permeability for organ health. This review outlines the basic characteristics and classes of biosurfactants (glycolipids, lipopeptides, phospholipids, and glycoproteins) and explores their biomedical importance, emphasizing their anti-adhesive, antimicrobial, and immune-modulating properties. This review aimed to provide outline the fundamental characteristics of biosurfactants and deliver a brief overview of their different classes, including glycolipids, lipopeptides, phospholipids, and glycoproteins. Furthermore, this review also explore their biomedical significance, highlighting their anti-adhesive, antimicrobial, and immune-modulating properties.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"297"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultivation of microalgae Chlorella vulgaris, Monoraphidium sp and Scenedesmus obliquus in wastewater from the household appliance industry for bioremediation and biofuel production. 在家用电器工业废水中培养微藻类小球藻、单胞藻和双胞藻,用于生物修复和生物燃料生产。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1007/s13205-024-04142-z
Kelly Lima de Oliveira, José Lucas da Silva Oliveira, Egídia Andrade Moraes, Kelma Maria Dos Santos Pires Cavalcante, Mona Lisa Moura de Oliveira, Carlúcio Roberto Alves

Microalgae Chlorella vulgaris, Scenedesmus obliquus, and Monoraphidium sp were cultivated in effluent from the household appliance industry as an alternative medium for bioremediation due to the high variability of chemical and biological substances in wastewater. The experiments were carried out using biological effluent (BE), chemical effluent (CE), and a combination of the two (MIX). The results showed a maximum biomass yield of 1056 mg/L (± 0.216) in the BE cultivation of the microalga Scenedesmus obliquus, 969 mg/L (± 0.20) in the BE of the microalga Monoraphidium sp. and 468 mg/L (± 0.46) in the CE of Chlorella vulgaris. In addition, they showed N O 3 - removal (100%) in the CE and MIX for cultivation with Chlorella vulgaris and 100% BE and 75% MIX with Monoraphidium sp. For the P O 3 4 - (75.3%, 99% e 97.9%) in the cultures with C. vulgaris BE, CE, and MIX respectively, with Monoraphidium sp. 58% in BE and 42% in CE and MIX. With S. obliquus, 100% removal was observed in all 3 treatments. Metal removal was also observed. The C. vulgaris culture showed lipid contents of 16%, 12%, and 17% for BE, CE, and MIX, respectively. For Monoraphidium sp., 14.5% for BE, 16% for CE, and 14% for MIX. In the culture of S. obliquus, 17%, 15.5%, and 16.5% for BE, CE, and MIX, respectively.

由于废水中的化学和生物物质变化很大,因此在家电行业的废水中培养了微藻类小球藻(Chlorella vulgaris)、钝顶藻(Scenedesmus obliquus)和藻单胞菌(Monoraphidium sp),作为生物修复的替代介质。实验使用了生物废水(BE)、化学废水(CE)和两者的组合(MIX)。结果表明,在 BE 中培养微藻 Scenedesmus obliquus 的最大生物量产量为 1056 mg/L(± 0.216),在 BE 中培养微藻 Monoraphidium sp.的最大生物量产量为 969 mg/L(± 0.20),在 CE 中培养 Chlorella vulgaris 的最大生物量产量为 468 mg/L(± 0.46)。此外,在培养绿藻的 CE 和 MIX 中,N O 3 - 的去除率为 100%;在培养单藻类的 BE 和 MIX 中,N O 3 - 的去除率分别为 100%和 75%;在培养绿藻的 BE、CE 和 MIX 中,P O 3 4 - 的去除率分别为 75.3%、99% 和 97.9%;在培养单藻类的 BE 中,去除率为 58%;在 CE 和 MIX 中,去除率为 42%。对于 S. obliquus,在所有 3 种处理中均观察到 100%的去除率。还观察到了金属去除率。在 BE、CE 和 MIX 中,C. vulgaris 培养物的脂质含量分别为 16%、12% 和 17%。而对于 Monoraphidium sp.,BE 为 14.5%,CE 为 16%,MIX 为 14%。在 S. obliquus 的培养物中,BE、CE 和 MIX 的脂质含量分别为 17%、15.5% 和 16.5%。
{"title":"Cultivation of microalgae <i>Chlorella vulgaris</i>, <i>Monoraphidium</i> sp and <i>Scenedesmus obliquus</i> in wastewater from the household appliance industry for bioremediation and biofuel production.","authors":"Kelly Lima de Oliveira, José Lucas da Silva Oliveira, Egídia Andrade Moraes, Kelma Maria Dos Santos Pires Cavalcante, Mona Lisa Moura de Oliveira, Carlúcio Roberto Alves","doi":"10.1007/s13205-024-04142-z","DOIUrl":"https://doi.org/10.1007/s13205-024-04142-z","url":null,"abstract":"<p><p>Microalgae <i>Chlorella vulgaris, Scenedesmus obliquus, and Monoraphidium</i> sp were cultivated in effluent from the household appliance industry as an alternative medium for bioremediation due to the high variability of chemical and biological substances in wastewater. The experiments were carried out using biological effluent (BE), chemical effluent (CE), and a combination of the two (MIX). The results showed a maximum biomass yield of 1056 mg/L (± 0.216) in the BE cultivation of the microalga <i>Scenedesmus obliquus,</i> 969 mg/L (± 0.20) in the BE of the microalga <i>Monoraphidium</i> sp. and 468 mg/L (± 0.46) in the CE of <i>Chlorella vulgaris.</i> In addition, they showed <math><mrow><mi>N</mi> <msubsup><mi>O</mi> <mrow><mn>3</mn></mrow> <mo>-</mo></msubsup> </mrow> </math> removal (100%) in the CE and MIX for cultivation with <i>Chlorella vulgaris</i> and 100% BE and 75% MIX with <i>Monoraphidium</i> sp<i>.</i> For the <math><mrow><mi>P</mi> <msubsup><mi>O</mi> <mrow><mn>3</mn></mrow> <mrow><mn>4</mn> <mo>-</mo></mrow> </msubsup> </mrow> </math> (75.3%, 99% e 97.9%) in the cultures with <i>C. vulgaris</i> BE, CE, and MIX respectively, with <i>Monoraphidium</i> sp. 58% in BE and 42% in CE and MIX. With <i>S. obliquus</i>, 100% removal was observed in all 3 treatments. Metal removal was also observed. The <i>C. vulgaris</i> culture showed lipid contents of 16%, 12%, and 17% for BE, CE, and MIX, respectively. For <i>Monoraphidium</i> sp., 14.5% for BE, 16% for CE, and 14% for MIX. In the culture of <i>S. obliquus,</i> 17%, 15.5%, and 16.5% for BE, CE, and MIX, respectively.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"294"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryopreservation of two-celled pollen: a model system for studying the cellular mechanisms of cryoinjury and recovery. 双细胞花粉的冷冻保存:研究冷冻损伤和恢复的细胞机制的模型系统。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.1007/s13205-024-04140-1
Ravi Gowthami, P E Rajasekharan, Subhash Chander, Muthusamy Shankar, Vartika Srivastava, Anuradha Agrawal

Cryopreservation serves as an invaluable technique for safeguarding the genetic diversity of plants and various organisms, while also facilitating fundamental biological research. Despite notable advancements in this field, the cryopreservation of certain cell types and tissues remains challenging, particularly those that exhibit sensitivity to low temperatures. Two-celled pollen is a promising model system for the study of cryopreservation. By exploring the cryopreservation of two-celled pollen, deeper insights can be gained into the cellular and molecular mechanisms of cryoinjury and recovery. This knowledge can be used to develop new and improved cryopreservation protocols for a wider range of cell types and tissues. It is relatively simple, consisting of only two cells, and it is relatively easy to cryopreserve and culture. In addition to its potential for improving cryopreservation technologies, the study of two-celled pollen cryopreservation can also shed light on fundamental biological processes such as cell division, development, and stress tolerance. By unlocking the mysteries of two-celled pollen cryopreservation, we can gain a deeper understanding of nature's inner workings. This article reviews examples of studies that have successfully used two-celled pollen cryopreservation, highlighting key findings and discoveries enabled by this technique as case studies.

低温冷冻技术是保护植物和各种生物遗传多样性的一项宝贵技术,同时也为基础生物学研究提供了便利。尽管在这一领域取得了显著进展,但某些细胞类型和组织的冷冻保存仍然具有挑战性,尤其是那些对低温敏感的细胞和组织。双细胞花粉是一种很有前景的低温保存研究模型系统。通过探索双细胞花粉的低温保存,可以深入了解低温损伤和恢复的细胞和分子机制。这些知识可用于为更广泛的细胞类型和组织开发新的和改进的低温保存方案。它的结构相对简单,只有两个细胞,冷冻保存和培养也相对容易。除了具有改进低温保存技术的潜力外,对双胞花粉低温保存的研究还能揭示基本的生物过程,如细胞分裂、发育和应激耐受性。通过揭开双细胞花粉低温保存的神秘面纱,我们可以更深入地了解大自然的内部运作。本文回顾了成功使用双细胞花粉冷冻保存技术的研究实例,并以案例研究的形式重点介绍了这项技术带来的主要发现和发现。
{"title":"Cryopreservation of two-celled pollen: a model system for studying the cellular mechanisms of cryoinjury and recovery.","authors":"Ravi Gowthami, P E Rajasekharan, Subhash Chander, Muthusamy Shankar, Vartika Srivastava, Anuradha Agrawal","doi":"10.1007/s13205-024-04140-1","DOIUrl":"https://doi.org/10.1007/s13205-024-04140-1","url":null,"abstract":"<p><p>Cryopreservation serves as an invaluable technique for safeguarding the genetic diversity of plants and various organisms, while also facilitating fundamental biological research. Despite notable advancements in this field, the cryopreservation of certain cell types and tissues remains challenging, particularly those that exhibit sensitivity to low temperatures. Two-celled pollen is a promising model system for the study of cryopreservation. By exploring the cryopreservation of two-celled pollen, deeper insights can be gained into the cellular and molecular mechanisms of cryoinjury and recovery. This knowledge can be used to develop new and improved cryopreservation protocols for a wider range of cell types and tissues. It is relatively simple, consisting of only two cells, and it is relatively easy to cryopreserve and culture. In addition to its potential for improving cryopreservation technologies, the study of two-celled pollen cryopreservation can also shed light on fundamental biological processes such as cell division, development, and stress tolerance. By unlocking the mysteries of two-celled pollen cryopreservation, we can gain a deeper understanding of nature's inner workings. This article reviews examples of studies that have successfully used two-celled pollen cryopreservation, highlighting key findings and discoveries enabled by this technique as case studies.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"304"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum. 利用腊菊水提取物绿色合成的氧化锌纳米颗粒的抗糖尿病、抗炎、抗氧化和细胞毒性潜力。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04125-0
Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju

The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of Helichrysum cymosum shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (p < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.

目前的研究涉及利用腊菊嫩枝的水提取物合成氧化锌纳米颗粒(ZnO-NPs),并随后通过不同的分析方法进行表征,如紫外可见光谱、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)、透射电子显微镜(TEM)和 zeta 电位。然后,通过一系列分析,包括细胞毒性、抗氧化、抗炎和酶抑制抗糖尿病作用,测试了 ZnO-NPs 对 C3A 肝细胞和 L6 肌细胞的生物效应。紫外可见光谱分析显示,该化合物在 360 波长处有最大吸收光谱;TEM 分析显示,该化合物呈球形和六边形结构,平均尺寸为 28.05-58.3 nm;XRD 显示,该化合物呈结晶六边形结构。zeta电位评估表明,ZnO-NPs在- 20 mV下相对稳定,傅立叶变换红外光谱分析确定了一些与酚类、羧酸和酰胺相关的重要官能团,它们是还原和稳定ZnO-NPs的主要成分。合成的 ZnO-NPs 在较高浓度(125 微克/毫升和 250 微克/毫升)时对细胞系有细胞毒性作用,这使得炎症和抗氧化检测结果的解释变得复杂。然而,在较高浓度下(125 微克/毫升和 250 微克/毫升),锰氧化物对细胞株的细胞毒性作用明显(p
{"title":"Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of <i>Helichrysum cymosum</i>.","authors":"Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju","doi":"10.1007/s13205-024-04125-0","DOIUrl":"10.1007/s13205-024-04125-0","url":null,"abstract":"<p><p>The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of <i>Helichrysum cymosum</i> shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (<i>p</i> < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"291"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dissecting the genetic diversity of cultivated tomato (Solanum lycopersicum) germplasm resources: a comparison of ddRADseq genotyping and microsatellite analysis via capillary electrophoresis and high-resolution melting. 剖析栽培番茄(Solanum lycopersicum)种质资源的遗传多样性:通过毛细管电泳和高分辨率熔融对 ddRADseq 基因分型和微卫星分析进行比较。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1007/s13205-024-04141-0
Pasquale Tripodi, Rosa D'Alessandro, Annalisa Cocozza, Gabriele Campanelli

Understanding the genetic diversity of crops is of fundamental importance for the efficient use and improvement of germplasm resources. Different molecular genotyping systems have been implemented for population structure and phylogenetic relationships analyses, among which, microsatellites (SSRs) and single nucleotide polymorphisms (SNPs) markers have been the most widely used. This study reports the efficacy of SNPs detected via double-digest restriction-site-associated DNA sequencing (ddRADseq) and SSRs analyzed via capillary electrophoresis (CE) and high-resolution melting (HRM) in tomato. In total, 21,020 high-quality SNPs, 20 CE-SSRs, and 17 HRM-SSR markers were assayed in a panel of 72 accessions that included a diversified set of landraces, long-shelf-life cultivars and heirlooms with different origins and fruit typology. The results showed how the population structure analysis was consistent using the three genotyping methods, although SNPs were more efficient in distinguishing cultivar types and in measuring the degree of accessions' similarity. Compared to CE-SSR, the analysis of microsatellites via HRM yielded a slightly higher number of alleles (98 vs. 96). HRM-SSR demonstrated a distinction between European and non-European germplasm, better resolving the collection's diversity and being more consistent with SNP data. Phylogenetic trees drawn with independent marker data, detected specific groups of accessions showing robust clusters, highlighting how heirlooms were less heterogeneous than landraces. In addition, the fixation index (F ST ) revealed a high genetic differentiation between heirlooms and long-shelf-life cultivars, with SNP and SSR-HRM data emphasizing the distinction between cherry and plum types and CE-SSR data between cherry and oxheart types. In all instances, a greater molecular variance was found within the different considered biological statuses, provenances, and typologies rather than among them. This work presents the first attempt to compare the three tomato genotyping techniques in tomato. Findings highlighted how the markers used are complementary for genetic diversity analysis, with SNPs providing better insight and HRM-SSR as a viable alternative to capillary electrophoresis to dissect the genetic structure.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04141-0.

了解作物的遗传多样性对于有效利用和改良种质资源至关重要。目前已有不同的分子基因分型系统用于种群结构和系统发育关系分析,其中微卫星(SSR)和单核苷酸多态性(SNPs)标记应用最为广泛。本研究报告了番茄中通过双酶切限制性位点相关 DNA 测序(ddRADseq)检测的 SNPs 以及通过毛细管电泳(CE)和高分辨率熔融(HRM)分析的 SSRs 的功效。在一个由 72 个品种组成的小组中,共检测了 21,020 个高质量 SNPs、20 个 CE-SSR 和 17 个 HRM-SSR 标记,这些品种包括不同来源和果实类型的多样化陆生品种、长货架期栽培品种和传家品种。结果表明,尽管 SNP 在区分栽培品种类型和衡量加入品系的相似程度方面更有效,但使用这三种基因分型方法进行的种群结构分析是一致的。与 CE-SSR 相比,通过 HRM 分析微卫星得到的等位基因数量略高(98 对 96)。HRM-SSR 显示了欧洲种质与非欧洲种质之间的区别,更好地解析了收集的多样性,并与 SNP 数据更加一致。利用独立标记数据绘制的系统发育树检测到了特定的入选种群,显示出强大的聚类,突出了传家宝的异质性低于陆生品系。此外,固定指数(F ST)显示了传家宝和长货架期栽培品种之间的高度遗传分化,SNP 和 SSR-HRM 数据强调了樱桃和李子类型之间的区别,而 CE-SSR 数据则强调了樱桃和牛心类型之间的区别。在所有情况下,不同生物状态、产地和类型之间的分子差异更大。这项研究首次尝试对三种番茄基因分型技术进行比较。研究结果凸显了所使用的标记在遗传多样性分析中的互补性,SNPs 提供了更好的洞察力,HRM-SSR 是毛细管电泳剖析遗传结构的可行替代方法:在线版本包含补充材料,可查阅 10.1007/s13205-024-04141-0。
{"title":"Dissecting the genetic diversity of cultivated tomato (<i>Solanum lycopersicum</i>) germplasm resources: a comparison of ddRADseq genotyping and microsatellite analysis via capillary electrophoresis and high-resolution melting.","authors":"Pasquale Tripodi, Rosa D'Alessandro, Annalisa Cocozza, Gabriele Campanelli","doi":"10.1007/s13205-024-04141-0","DOIUrl":"https://doi.org/10.1007/s13205-024-04141-0","url":null,"abstract":"<p><p>Understanding the genetic diversity of crops is of fundamental importance for the efficient use and improvement of germplasm resources. Different molecular genotyping systems have been implemented for population structure and phylogenetic relationships analyses, among which, microsatellites (SSRs) and single nucleotide polymorphisms (SNPs) markers have been the most widely used. This study reports the efficacy of SNPs detected via double-digest restriction-site-associated DNA sequencing (ddRADseq) and SSRs analyzed via capillary electrophoresis (CE) and high-resolution melting (HRM) in tomato. In total, 21,020 high-quality SNPs, 20 CE-SSRs, and 17 HRM-SSR markers were assayed in a panel of 72 accessions that included a diversified set of landraces, long-shelf-life cultivars and heirlooms with different origins and fruit typology. The results showed how the population structure analysis was consistent using the three genotyping methods, although SNPs were more efficient in distinguishing cultivar types and in measuring the degree of accessions' similarity. Compared to CE-SSR, the analysis of microsatellites via HRM yielded a slightly higher number of alleles (98 vs<i>.</i> 96). HRM-SSR demonstrated a distinction between European and non-European germplasm, better resolving the collection's diversity and being more consistent with SNP data. Phylogenetic trees drawn with independent marker data, detected specific groups of accessions showing robust clusters, highlighting how heirlooms were less heterogeneous than landraces. In addition, the fixation index (<i>F</i> <sub><i>ST</i></sub> ) revealed a high genetic differentiation between heirlooms and long-shelf-life cultivars, with SNP and SSR-HRM data emphasizing the distinction between cherry and plum types and CE-SSR data between cherry and oxheart types. In all instances, a greater molecular variance was found within the different considered biological statuses, provenances, and typologies rather than among them. This work presents the first attempt to compare the three tomato genotyping techniques in tomato. Findings highlighted how the markers used are complementary for genetic diversity analysis, with SNPs providing better insight and HRM-SSR as a viable alternative to capillary electrophoresis to dissect the genetic structure.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04141-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"296"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Micropropagation and in vitro rejuvenation of Eucalyptus cloeziana F. Muell. 桉树的微繁殖和离体再生
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-06 DOI: 10.1007/s13205-024-04131-2
Leandro Silva de Oliveira, Douglas Machado Leite, Fabíola Magalhães Mendes, Letícia Vaz Molinari, Gilvano Ebling Brondani, Antônio Natal Gonçalves, Marcilio de Almeida

Micropropagation is an important tool for the propagation for recalcitrant species, like Eucalyptus cloeziana. This is a first reported about a complete study of the E. cloeziana, it includes the vegetative rescue protocol of mature trees, its micropropagation up to the implementation of a mini-garden and clonal micro-garden and evaluation of adventitious rooting of their propagules. The in vitro rejuvenation of E. cloeziana genotypes (03, 05, 06, 13 and 15), over 21 successive subcultures, was evaluated by comparing minicutting and microcutting techniques and the efficiency of this biotechnological tool for cloning of the mature trees. The results provide evidence of the in vitro rejuvenation occurring in the mature E. cloeziana trees during micropropagation, leading to increases in the multiplication ratios of the microstumps of three genotypes (03, 05 and 15). The survival and callogenesis percentages of the mini and microcuttings of E. cloeziana were higher than 80% upon exiting of the greenhouse (30-days old) in the three cutting times. The results suggesting that factors beyond tissue maturity influence the adventitious rooting of the E. cloeziana because minicuttings and microcuttings of the 05 and 13 elite genotypes exhibited rooting percentages lower than 20%. These findings provided a theoretical basis for realizing the micropropagation of the epicormic shoots of the mature trees and, subsequently, its in vitro rejuvenation by axillary bud proliferation.

微繁殖是繁殖桉树等顽固树种的重要工具。本文首次报道了对无花果桉树的完整研究,包括成熟树木的无性繁殖拯救方案、微繁殖直至小型花园和克隆微花园的实施,以及对其繁殖体不定根的评估。通过比较微型栽培和微切技术,评估了 E. cloeziana 基因型(03、05、06、13 和 15)在连续 21 次亚培养过程中的离体再生情况,以及这种生物技术手段在克隆成熟树木方面的效率。结果证明,在微繁殖过程中,成熟的 E. cloeziana 树体发生了离体再生,导致三个基因型(03、05 和 15)的微桩繁殖率增加。在三个扦插时间中,小插穗和微插穗在离开温室(30 天)时的存活率和胼胝发生率均高于 80%。由于 05 和 13 个精英基因型的迷你插条和微插条的生根率均低于 20%,这些结果表明组织成熟度以外的因素会影响 E. cloeziana 的不定根。这些发现为实现成熟树木外植体芽的微繁殖,以及随后通过腋芽增殖使其在体外返老还童提供了理论依据。
{"title":"Micropropagation and in vitro rejuvenation of <i>Eucalyptus cloeziana</i> F. Muell.","authors":"Leandro Silva de Oliveira, Douglas Machado Leite, Fabíola Magalhães Mendes, Letícia Vaz Molinari, Gilvano Ebling Brondani, Antônio Natal Gonçalves, Marcilio de Almeida","doi":"10.1007/s13205-024-04131-2","DOIUrl":"https://doi.org/10.1007/s13205-024-04131-2","url":null,"abstract":"<p><p>Micropropagation is an important tool for the propagation for recalcitrant species, like <i>Eucalyptus cloeziana.</i> This is a first reported about a complete study of the <i>E. cloeziana</i>, it includes the vegetative rescue protocol of mature trees, its micropropagation up to the implementation of a mini-garden and clonal micro-garden and evaluation of adventitious rooting of their propagules. The in vitro rejuvenation of <i>E. cloeziana</i> genotypes (03, 05, 06, 13 and 15), over 21 successive subcultures, was evaluated by comparing minicutting and microcutting techniques and the efficiency of this biotechnological tool for cloning of the mature trees. The results provide evidence of the in vitro rejuvenation occurring in the mature <i>E. cloeziana</i> trees during micropropagation, leading to increases in the multiplication ratios of the microstumps of three genotypes (03, 05 and 15). The survival and callogenesis percentages of the mini and microcuttings of <i>E. cloeziana</i> were higher than 80% upon exiting of the greenhouse (30-days old) in the three cutting times. The results suggesting that factors beyond tissue maturity influence the adventitious rooting of the <i>E. cloeziana</i> because minicuttings and microcuttings of the 05 and 13 elite genotypes exhibited rooting percentages lower than 20%. These findings provided a theoretical basis for realizing the micropropagation of the epicormic shoots of the mature trees and, subsequently, its in vitro rejuvenation by axillary bud proliferation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"292"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanomaterial-enabled drug transport systems: a comprehensive exploration of current developments and future avenues in therapeutic delivery. 纳米材料药物传输系统:全面探索治疗传输的当前发展和未来途径。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04135-y
Shatabdi Basu, Pragnya Biswas, Mariya Anto, Nandini Singh, Koel Mukherjee

Over the years, nanotechnology has gained popularity as a viable solution to address gene and drug delivery challenges over conventional methods. Extensive research has been conducted on nanosystems that consist of organic/inorganic materials, drugs, and its biocompatibility become the primary goal of improving drug delivery. Various surface modification methods help focus targeted and controlled drug release, further enabling multidrug delivery also. This newer technology ensures the stability of drugs that can unravel the mechanisms involved in cellular processes of disease development and its management. Tailored medication delivery provides benefits such as therapy, controlled release, and reduced adverse effects, which are especially important for controlling illnesses like cancer. However, multifunctional nanocarriers that possess high viscoelasticity, extended circulation half-life, biocompatibility, and biodegradability face some challenges and limitations too in human bodies. To produce a consistent therapeutic platform based on complex three-dimensional nanoparticles, careful design and engineering, thorough orthogonal analysis methods, and reproducible scale-up and manufacturing processes will be required in the future. Safety and effectiveness of nano-based drug delivery should be thoroughly investigated in preclinical and clinical trials, especially when considering biodistribution, targeting specific areas, and potential immunological toxicities. Overall, the current review article explores the advancements in nanotechnology, specific to nanomaterial-enabled drug delivery systems, carrier fabrication techniques and modifications, disease management, clinical research, applications, limitations, and future challenges. The work portrays how nanomedicine distribution affects healthcare with an emphasis on the developments in drug delivery techniques.

多年来,与传统方法相比,纳米技术作为解决基因和药物递送难题的一种可行方案,已逐渐受到人们的青睐。人们对由有机/无机材料、药物及其生物相容性组成的纳米系统进行了广泛的研究,并将其作为改善药物输送的首要目标。各种表面改性方法有助于有针对性地控制药物释放,进一步实现多药给药。这种更新的技术可确保药物的稳定性,从而揭示疾病发生和治疗的细胞过程所涉及的机制。量身定制的给药方式具有治疗、控制释放和减少不良反应等优点,这对于控制癌症等疾病尤为重要。然而,具有高粘弹性、延长循环半衰期、生物相容性和生物降解性的多功能纳米载体在人体中也面临着一些挑战和限制。要生产出基于复杂三维纳米颗粒的稳定治疗平台,未来需要精心设计和工程设计、全面的正交分析方法以及可重复的放大和制造工艺。应在临床前和临床试验中对纳米给药的安全性和有效性进行彻底研究,尤其是在考虑生物分布、靶向特定区域和潜在免疫毒性时。总之,本综述文章探讨了纳米技术的发展,特别是纳米材料给药系统、载体制造技术和改性、疾病管理、临床研究、应用、局限性和未来挑战。文章以给药技术的发展为重点,描绘了纳米医学的分布如何影响医疗保健。
{"title":"Nanomaterial-enabled drug transport systems: a comprehensive exploration of current developments and future avenues in therapeutic delivery.","authors":"Shatabdi Basu, Pragnya Biswas, Mariya Anto, Nandini Singh, Koel Mukherjee","doi":"10.1007/s13205-024-04135-y","DOIUrl":"10.1007/s13205-024-04135-y","url":null,"abstract":"<p><p>Over the years, nanotechnology has gained popularity as a viable solution to address gene and drug delivery challenges over conventional methods. Extensive research has been conducted on nanosystems that consist of organic/inorganic materials, drugs, and its biocompatibility become the primary goal of improving drug delivery. Various surface modification methods help focus targeted and controlled drug release, further enabling multidrug delivery also. This newer technology ensures the stability of drugs that can unravel the mechanisms involved in cellular processes of disease development and its management. Tailored medication delivery provides benefits such as therapy, controlled release, and reduced adverse effects, which are especially important for controlling illnesses like cancer. However, multifunctional nanocarriers that possess high viscoelasticity, extended circulation half-life, biocompatibility, and biodegradability face some challenges and limitations too in human bodies. To produce a consistent therapeutic platform based on complex three-dimensional nanoparticles, careful design and engineering, thorough orthogonal analysis methods, and reproducible scale-up and manufacturing processes will be required in the future. Safety and effectiveness of nano-based drug delivery should be thoroughly investigated in preclinical and clinical trials, especially when considering biodistribution, targeting specific areas, and potential immunological toxicities. Overall, the current review article explores the advancements in nanotechnology, specific to nanomaterial-enabled drug delivery systems, carrier fabrication techniques and modifications, disease management, clinical research, applications, limitations, and future challenges. The work portrays how nanomedicine distribution affects healthcare with an emphasis on the developments in drug delivery techniques.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"289"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant resources for immunonutrients and immunomodulators to combat infectious respiratory viral diseases: a review. 抗击传染性呼吸道病毒疾病的免疫营养素和免疫调节剂植物资源:综述。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-16 DOI: 10.1007/s13205-024-04143-y
Sobha Kota, Anand Kumar Nelapati, Vayunandana Rao Govada

Boosting the immune system has become a crucial aspect in the global battle against the COVID-19 pandemic and other similar infections to protect oneself against symptoms, especially in the prevention of viral infections of the lower respiratory tract. The importance of conducting more studies to create successful herbal formulations as infection prevention measures is emphasized in this review, which looks at the function of immune-boosting nutrients, medicinal plants, and herbal treatments. We reviewed and analyzed 207 studies published from 1946 to the present using reputable databases like Google Scholar, PubMed, and NCBI. The review examined 115 plant species in total and identified 12 key nutrients, including vitamins A, D, C, omega-3 fatty acids, iron, and zinc, while noting that four plant families, Rosaceae, Asteraceae, Amaryllidaceae, and Acanthaceae, show potential against respiratory infections like influenza, RSV, and SARS-CoV. To lower the risk of infection, it is recommended to consume nutritious meals that have immune-modulating qualities. Information on the bioactive components of medicinal herbs, spices, and plants that have been effective in treating respiratory viral infections and related conditions is compiled in this review, which highlights phytoactive substances with antibacterial and antiviral activity as effective modulators to lower the risk of infections. Furthermore, it is highlighted that ancient knowledge systems, like Ayurveda and Naturopathy, should be integrated to help develop new herbal formulations. To improve immunity and lessen vulnerability to serious respiratory infections, the results highlight the need for including immune-modulating foods and plant-based medicines into everyday routines.

在全球抗击 COVID-19 大流行病和其他类似感染的斗争中,增强免疫系统已成为保护自身免受症状困扰的一个重要方面,尤其是在预防下呼吸道病毒感染方面。本综述探讨了提高免疫力的营养素、药用植物和草药疗法的功能,强调了开展更多研究以成功研制草药配方作为预防感染措施的重要性。我们使用 Google Scholar、PubMed 和 NCBI 等著名数据库,对 1946 年至今发表的 207 项研究进行了回顾和分析。综述共研究了 115 种植物,并确定了 12 种关键营养素,包括维生素 A、D、C、欧米茄-3 脂肪酸、铁和锌,同时指出蔷薇科、菊科、金莲花科和刺桐科这四个植物科对流感、RSV 和 SARS-CoV 等呼吸道感染具有潜在的抵抗力。为降低感染风险,建议食用具有免疫调节功能的营养膳食。本综述汇编了在治疗呼吸道病毒感染和相关疾病方面有效的药草、香料和植物的生物活性成分信息,重点介绍了具有抗菌和抗病毒活性的植物活性物质,它们是降低感染风险的有效调节剂。此外,综述还强调,应结合阿育吠陀和自然疗法等古老知识体系,帮助开发新的草药配方。为了提高免疫力,降低易患严重呼吸道感染的风险,研究结果强调有必要将免疫调节食品和植物性药物纳入日常生活中。
{"title":"Plant resources for immunonutrients and immunomodulators to combat infectious respiratory viral diseases: a review.","authors":"Sobha Kota, Anand Kumar Nelapati, Vayunandana Rao Govada","doi":"10.1007/s13205-024-04143-y","DOIUrl":"10.1007/s13205-024-04143-y","url":null,"abstract":"<p><p>Boosting the immune system has become a crucial aspect in the global battle against the COVID-19 pandemic and other similar infections to protect oneself against symptoms, especially in the prevention of viral infections of the lower respiratory tract. The importance of conducting more studies to create successful herbal formulations as infection prevention measures is emphasized in this review, which looks at the function of immune-boosting nutrients, medicinal plants, and herbal treatments. We reviewed and analyzed 207 studies published from 1946 to the present using reputable databases like Google Scholar, PubMed, and NCBI. The review examined 115 plant species in total and identified 12 key nutrients, including vitamins A, D, C, omega-3 fatty acids, iron, and zinc, while noting that four plant families, Rosaceae, Asteraceae, Amaryllidaceae, and Acanthaceae, show potential against respiratory infections like influenza, RSV, and SARS-CoV. To lower the risk of infection, it is recommended to consume nutritious meals that have immune-modulating qualities. Information on the bioactive components of medicinal herbs, spices, and plants that have been effective in treating respiratory viral infections and related conditions is compiled in this review, which highlights phytoactive substances with antibacterial and antiviral activity as effective modulators to lower the risk of infections. Furthermore, it is highlighted that ancient knowledge systems, like Ayurveda and Naturopathy, should be integrated to help develop new herbal formulations. To improve immunity and lessen vulnerability to serious respiratory infections, the results highlight the need for including immune-modulating foods and plant-based medicines into everyday routines.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"302"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metagenomics approaches in the discovery and development of new bioactive compound of 8-demethoxy-10-deoxysteffimycin from mangrove sediments. 利用元基因组学方法从红树林沉积物中发现和开发新的生物活性化合物 8-去甲氧基-10-脱氧半胱氨酸。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-17 DOI: 10.1007/s13205-024-04137-w
P Sujith, J Rajesh Singh, S Jayalakshmi, Karthikeyan Kandaswamy, Mohammed Rafi Shaik, Shaik Althaf Hussain, Zulhisyam Abdul Kari, Ajay Guru

A metagenomic library consisting of 15,000 clones was constructed from the mangrove sediment. An antimicrobially active clone from the metagenomic library PS49 was identified by function- based screening. This paper presents the results of the biochemical characterization and metagenomic library screening of the marine-derived antibiotic, 8-demethoxy-10-deoxysteffimycin. Plasmid libraries were constructed, and clones were produced using a metagenomic approach. Out of 15,000 clones, 81 clones were screened for antimicrobial activity, and five potential clones were selected. The activity of one clone was characterized and named as PS49. The bioactive compounds from the selected clone were checked for antimicrobial, antioxidant, and anticancer activities. The clone PS49 was tested against various pathogens including bacteria and fungi and it showed inhibitory effects against all the tested pathogens. The antimicrobially active fractions were then crystallized and subjected to spectroscopic analysis such as FTIR, NMR and LC-MS analysis. The substance from clone PS49 has finally been recognized, and the compound from clone PS49 has been identified as 8-demethoxy-10-deoxysteffimycin. The substances isolated from the PS49 clone exhibited strong anticancer activity against skin cancer-cell lines SK-MEL2. The compounds showed a reduction in cell viability with an increase in the compound concentration. The compounds obtained from clone PS49 showed an IC50 value of 85 µg/ml.

从红树林沉积物中构建了一个由 15,000 个克隆组成的元基因组文库。通过基于功能的筛选,从元基因组文库 PS49 中鉴定出了一个具有抗菌活性的克隆。本文介绍了海洋衍生抗生素 8-去甲氧基-10-脱氧半胱氨酸的生化特征和元基因组文库筛选结果。本文构建了质粒文库,并使用元基因组方法产生了克隆。在 15,000 个克隆中,对 81 个克隆进行了抗菌活性筛选,选出了 5 个潜在克隆。对其中一个克隆的活性进行了鉴定,并将其命名为 PS49。对所选克隆中的生物活性化合物进行了抗菌、抗氧化和抗癌活性检测。对克隆 PS49 进行了针对各种病原体(包括细菌和真菌)的测试,结果表明它对所有受测病原体都有抑制作用。随后,抗菌活性馏分被结晶化,并进行了傅立叶变换红外光谱、核磁共振和液相色谱-质谱分析等光谱分析。最终确认了克隆 PS49 中的物质,并确定克隆 PS49 中的化合物为 8-去甲氧基-10-脱氧半胱氨酸。从 PS49 克隆中分离出的物质对皮肤癌细胞株 SK-MEL2 具有很强的抗癌活性。这些化合物显示,随着化合物浓度的增加,细胞存活率降低。从克隆 PS49 中获得的化合物的 IC50 值为 85 µg/ml。
{"title":"Metagenomics approaches in the discovery and development of new bioactive compound of 8-demethoxy-10-deoxysteffimycin from mangrove sediments.","authors":"P Sujith, J Rajesh Singh, S Jayalakshmi, Karthikeyan Kandaswamy, Mohammed Rafi Shaik, Shaik Althaf Hussain, Zulhisyam Abdul Kari, Ajay Guru","doi":"10.1007/s13205-024-04137-w","DOIUrl":"10.1007/s13205-024-04137-w","url":null,"abstract":"<p><p>A metagenomic library consisting of 15,000 clones was constructed from the mangrove sediment. An antimicrobially active clone from the metagenomic library PS49 was identified by function- based screening. This paper presents the results of the biochemical characterization and metagenomic library screening of the marine-derived antibiotic, 8-demethoxy-10-deoxysteffimycin. Plasmid libraries were constructed, and clones were produced using a metagenomic approach. Out of 15,000 clones, 81 clones were screened for antimicrobial activity, and five potential clones were selected. The activity of one clone was characterized and named as PS49. The bioactive compounds from the selected clone were checked for antimicrobial, antioxidant, and anticancer activities. The clone PS49 was tested against various pathogens including bacteria and fungi and it showed inhibitory effects against all the tested pathogens. The antimicrobially active fractions were then crystallized and subjected to spectroscopic analysis such as FTIR, NMR and LC-MS analysis. The substance from clone PS49 has finally been recognized, and the compound from clone PS49 has been identified as 8-demethoxy-10-deoxysteffimycin. The substances isolated from the PS49 clone exhibited strong anticancer activity against skin cancer-cell lines SK-MEL2. The compounds showed a reduction in cell viability with an increase in the compound concentration. The compounds obtained from clone PS49 showed an IC<sub>50</sub> value of 85 µg/ml.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"303"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Xihuang Pill in inducing pyroptosis in glioma cells through modulation of miR-21-5p. 通过调节 miR-21-5p 评估西黄丸诱导胶质瘤细胞热休克的作用
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1007/s13205-024-04148-7
Ning Tang, Nuojin Geng, Xinhua Zhu

This study aims to elucidate the mechanism by which Xihuang Pill induces pyroptosis in glioma cells via the regulation of miR-21-5p. Human glioma cell lines U-87 and LN-229 were used as experimental models to assess the effects of Xihuang Pill on glioma pyroptosis. Cells were incubated with Xihuang Pill extract at concentrations of 7.5, 15, and 30 µg/mL for 24 h, alongside transfection with miR-21-5p mimic, an overexpression vector for STAT3, or incubation with 50 µg/mL of the STAT3 activator Colivelin for 4 h. Cell viability was measured using the CCK-8 assay, apoptosis was detected by flow cytometry, and expression levels of p-STAT3/STAT3 and pyroptosis-related proteins were determined by Western Blot. Additionally, cleaved caspase-1 was assessed by immunofluorescence, miR-21-5p expression by qRT-PCR, and STAT3 binding to the miR-21-5p promoter region by ChIP and dual-luciferase reporter assays. Results showed that Xihuang Pill significantly reduced cell viability, increased apoptosis, and upregulated the expression of pyroptosis-related proteins such as NLRP3, IL-1β, cleaved caspase-1, and GSDMD-N, while reducing p-STAT3/STAT3 and miR-21-5p levels (P < 0.05). Xihuang Pill inhibited STAT3 activation, which modulated miR-21-5p expression by binding to its promoter region. Co-transfection with miR-21-5p mimic reversed the effect of Xihuang Pill on glioma pyroptosis (P < 0.05). In conclusion, Xihuang Pill promotes glioma cell pyroptosis through the STAT3/miR-21-5p pathway.

本研究旨在阐明西黄丸通过调控miR-21-5p诱导胶质瘤细胞热休克的机制。以人胶质瘤细胞株U-87和LN-229为实验模型,评估西黄丸对胶质瘤热休克的影响。用浓度为7.5、15和30微克/毫升的西黄丸提取物培养细胞24小时,同时转染miR-21-5p模拟物、STAT3过表达载体或用50微克/毫升的STAT3激活剂Colivelin培养4小时。细胞活力用 CCK-8 法测定,细胞凋亡用流式细胞术检测,p-STAT3/STAT3 和热凋亡相关蛋白的表达水平用 Western 印迹法测定。此外,免疫荧光法检测了裂解的caspase-1,qRT-PCR法检测了miR-21-5p的表达,ChIP和双荧光素酶报告实验检测了STAT3与miR-21-5p启动子区域的结合。结果表明,西黄丸能明显降低细胞活力,增加细胞凋亡,上调NLRP3、IL-1β、裂解的caspase-1和GSDMD-N等细胞凋亡相关蛋白的表达,同时降低p-STAT3/STAT3和miR-21-5p水平(P<0.05)。
{"title":"Evaluation of Xihuang Pill in inducing pyroptosis in glioma cells through modulation of miR-21-5p.","authors":"Ning Tang, Nuojin Geng, Xinhua Zhu","doi":"10.1007/s13205-024-04148-7","DOIUrl":"https://doi.org/10.1007/s13205-024-04148-7","url":null,"abstract":"<p><p>This study aims to elucidate the mechanism by which Xihuang Pill induces pyroptosis in glioma cells via the regulation of miR-21-5p. Human glioma cell lines U-87 and LN-229 were used as experimental models to assess the effects of Xihuang Pill on glioma pyroptosis. Cells were incubated with Xihuang Pill extract at concentrations of 7.5, 15, and 30 µg/mL for 24 h, alongside transfection with miR-21-5p mimic, an overexpression vector for STAT3, or incubation with 50 µg/mL of the STAT3 activator Colivelin for 4 h. Cell viability was measured using the CCK-8 assay, apoptosis was detected by flow cytometry, and expression levels of p-STAT3/STAT3 and pyroptosis-related proteins were determined by Western Blot. Additionally, cleaved caspase-1 was assessed by immunofluorescence, miR-21-5p expression by qRT-PCR, and STAT3 binding to the miR-21-5p promoter region by ChIP and dual-luciferase reporter assays. Results showed that Xihuang Pill significantly reduced cell viability, increased apoptosis, and upregulated the expression of pyroptosis-related proteins such as NLRP3, IL-1β, cleaved caspase-1, and GSDMD-N, while reducing p-STAT3/STAT3 and miR-21-5p levels (P < 0.05). Xihuang Pill inhibited STAT3 activation, which modulated miR-21-5p expression by binding to its promoter region. Co-transfection with miR-21-5p mimic reversed the effect of Xihuang Pill on glioma pyroptosis (P < 0.05). In conclusion, Xihuang Pill promotes glioma cell pyroptosis through the STAT3/miR-21-5p pathway.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"295"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
3 Biotech
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1