Faster liquid chromatography-tandem mass spectrometry method for analysis of isomeric urinary mercapturic acid metabolites of crotonaldehyde, methacrolein, and methyl vinyl ketone

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2024-09-19 DOI:10.1016/j.jchromb.2024.124324

Menglan Chen, Steven G. Carmella, Yingchun Zhao, Stephen S. Hecht

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Abstract

We report a significantly more rapid method for quantitation of the urinary mercapturic acids of the isomeric toxicants crotonaldehyde, methacrolein, and methyl vinyl ketone. The major innovation of this novel method is detection by liquid chromatography-negative atmospheric pressure chemical ionization-tandem mass spectrometry-selected reaction monitoring as opposed to detection by negative electrospray ionization in our previously reported method. The new method also uses an improved Raptor Biphenyl HPLC column. The total chromatographic analysis time was reduced to about 8 min compared to 35 min in our previously published method. Accuracy, precision, and ruggedness of the new method were established, and its suitability for the analysis of urine samples from 2500 cigarette smokers and non-smokers was confirmed. The improved method is practical for quantitation of these important toxicants in clinical studies requiring analysis of thousands of urine samples.

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更快的液相色谱-串联质谱法分析巴豆醛、甲基丙烯醛和甲基乙烯酮的异构体尿巯基酸代谢物。

我们报告了一种更快速的方法，用于定量检测尿液中巴豆醛、甲基丙烯醛和甲基乙烯酮异构毒物的巯基酸。这种新方法的主要创新点是通过液相色谱-负压化学电离-串联质谱-选择反应监测进行检测，而不是我们以前报告的通过负电喷雾电离进行检测。新方法还使用了改进的 Raptor 联苯高效液相色谱柱。色谱分析的总时间缩短至 8 分钟左右，而我们之前公布的方法需要 35 分钟。新方法的准确度、精密度和耐用性均得到了证实，并确认其适用于分析 2500 名吸烟者和非吸烟者的尿样。改进后的方法适用于需要分析数千份尿样的临床研究中这些重要毒物的定量分析。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.