Polymorphisms in the genes encoding RLR and TLR3 and CMV DNAemia in subjects coinfected with human immunodeficiency virus and cytomegalovirus

IF 2.5 4区 医学 Q3 VIROLOGY Archives of Virology Pub Date : 2024-09-27 DOI:10.1007/s00705-024-06114-3
Agnieszka Jabłońska, Elżbieta Jabłonowska, Mirosława Studzińska, Juliusz Kamerys, Edyta Paradowska
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Abstract

Cytomegalovirus (CMV) is a pathogen that is common worldwide and is often present in individuals infected with human immunodeficiency virus (HIV). Pattern recognition receptors (PRRs) are host sensors that activate the immune response against infectious agents. However, it is unclear whether PRR single-nucleotide polymorphisms (SNPs) are associated with the occurrence of CMV DNAemia in subjects coinfected with HIV and CMV. HIV/CMV-coinfected patients with and without CMV DNAemia were recruited for this study. The DDX58 rs10813831 and IFIH1 (rs3747517 and rs1990760) polymorphisms were genotyped using the TaqMan Allelic Discrimination Assay, whereas the DDX58 rs12006123 and TLR3 (rs3775291 and rs3775296) SNPs were analyzed using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. A mutation present in at least one allele of the DDX58 rs12006123 SNP occurred at least two times more frequently in HIV/CMV-coinfected patients with CMV DNAemia than in coinfected subjects without CMV DNAemia (OR, 2.50; 95% CI, 1.33–4.68; p = 0.004, in the dominant model). A higher level of CMV DNAemia was observed in subjects who had the heterozygous (GA) or homozygous recessive (AA) genotype for the DDX58 rs12006123 SNP compared with those who had the wild-type (GG) genotype (p = 0.0003). Moreover, in subjects with a mutation detected in at least one allele of the DDX58 rs12006123 SNP, a lower serum IFN-β concentration was found compared with those who had a wild-type (GG) genotype for this polymorphism (p = 0.024). The DDX58 rs12006123 SNP is associated with CMV DNAemia in HIV/CMV-coinfected patients.

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人类免疫缺陷病毒和巨细胞病毒双重感染者中编码 RLR 和 TLR3 基因的多态性与 CMV DNA 血症。
巨细胞病毒(CMV)是一种全球常见的病原体,感染了人类免疫缺陷病毒(HIV)的人体内经常会出现这种病毒。模式识别受体(PRRs)是宿主的感应器,可激活针对传染性病原体的免疫反应。然而,目前还不清楚 PRR 单核苷酸多态性(SNPs)是否与合并感染 HIV 和 CMV 的受试者发生 CMV DNA 血症有关。本研究招募了患有和未患有 CMV DNA 血症的 HIV/CMV 共同感染患者。使用 TaqMan Allelic Discrimination Assay 对 DDX58 rs10813831 和 IFIH1(rs3747517 和 rs1990760)多态性进行了基因分型,而 DDX58 rs12006123 和 TLR3(rs3775291 和 rs3775296)SNP 则使用聚合酶链反应限制性片段长度多态性(PCR-RFLP)检测法进行了分析。在患有 CMV DNA 血症的 HIV/CMV 共同感染患者中,DDX58 rs12006123 SNP 的至少一个等位基因出现突变的频率是没有 CMV DNA 血症的共同感染者的至少两倍(OR,2.50;95% CI,1.33-4.68;p = 0.004,显性模型)。与野生型(GG)基因型的受试者相比,DDX58 rs12006123 SNP 基因型为杂合型(GA)或同源隐性(AA)的受试者的 CMV DNA 血症水平更高(p = 0.0003)。此外,在 DDX58 rs12006123 SNP 的至少一个等位基因中检测到突变的受试者中,发现与该多态性的野生型(GG)基因型的受试者相比,血清 IFN-β 浓度较低(p = 0.024)。DDX58 rs12006123 SNP与HIV/CMV合并感染患者的CMV DNA血症有关。
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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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