Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI:10.1080/17576180.2024.2404383

Mariana Smidt, Marcos Frank Bastiani, Roberta Zilles Hahn, Lilian de Lima Feltraco Lizot, Amanda Pacheco Bondan, Giovana Piva Peteffi, Náthaly Cbf Dos Santos, José Luiz da Costa, Rafael Lanaro, Rafael Linden

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Abstract

Aim: Identifying drugs of abuse and their metabolites in plasma is vital in both forensic and clinical toxicology. While the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method offers an efficient approach to sample preparation, its application is complex due to the wide-ranging properties of target analytes and the challenges posed by biological matrix interferences. This study aims to develop a microQuEChERS approach for the quantification of 14 drugs of abuse and metabolites utilizing minimal sample and solvent volumes.Methods: The microQuEChERS method involved using 10 μl plasma samples, 25 mg of a salt mixture and 150 μl of acetonitrile. Extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), with a 7.5 min run. The assay was validated according to bioanalytical guidelines.Results: The accuracy was 96.8-112.4%. The within-assay precision was within 2.0-8.9% and the between-assay precision was within 3.2-8.2%. Matrix effects were found to range from -5.7 to 13.5%. The extraction yield was higher than 74.7%.Conclusion: This study described a microQuEChERS sample preparation approach for determining drugs of abuse and metabolites using plasma microsamples and LC-MS/MS. The approach is efficient, environmentally friendly and suitable for scenarios with limited amounts of biological samples.

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微量 QuEChERS 萃取后通过 LC-MS/MS 测定血浆微量样本中的滥用药物和代谢物。

目的：鉴别血浆中的滥用药物及其代谢物对法医和临床毒理学都至关重要。QuEChERS（快速、简便、廉价、有效、坚固和安全）方法提供了一种高效的样品制备方法，但由于目标分析物的各种特性以及生物基质干扰带来的挑战，该方法的应用非常复杂。本研究旨在开发一种微型 QuEChERS 方法，利用最小的样品和溶剂用量对 14 种滥用药物和代谢物进行定量分析：微量 QuEChERS 方法包括使用 10 μl 血浆样品、25 毫克盐混合物和 150 μl 乙腈。提取物采用液相色谱-串联质谱法（LC-MS/MS）进行分析，运行时间为 7.5 分钟。根据生物分析指南对该检测方法进行了验证：结果：准确度为 96.8%-112.4%。测定内精密度为 2.0-8.9%，测定间精密度为 3.2-8.2%。基质效应为-5.7%至13.5%。萃取率高于 74.7%：本研究介绍了一种利用血浆微量样品和 LC-MS/MS 测定药物滥用和代谢物的 microQuEChERS 样品制备方法。该方法高效、环保，适用于生物样本量有限的情况。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.