Simultaneous high-throughput particle-bacteria separation and solution exchange via in-plane and out-of-plane parallelization of microfluidic centrifuges.

IF 2.6 4区 工程技术 Q2 BIOCHEMICAL RESEARCH METHODS Biomicrofluidics Pub Date : 2024-09-23 eCollection Date: 2024-09-01 DOI:10.1063/5.0215930
Nima Norouzy, Alireza Zabihihesari, Pouya Rezai
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Abstract

Inertial microfluidic devices have gained attention for point-of-need (PoN) sample preparation. Yet, devices capable of simultaneous particle-bacteria solution exchange and separation are low in throughput, hindering their applicability to PoN settings. This paper introduces a microfluidic centrifuge for high-throughput solution exchange and separation of microparticles, addressing the need for processing large sample volumes at elevated flow rates. The device integrates Dean flow recirculation and inertial focusing of microparticles within 24 curved microchannels assembled in a three-layer configuration via in-plane and out-of-plane parallelization. We studied solution exchange and particle migration using singleplex and duplex samples across devices with varying curve numbers (2-curve, 8-curve, and 24-curve). Processing 5 and 10 μm microparticles at flow rates up to 16.8 ml/min achieved a solution exchange efficiency of 96.69%. In singleplex solutions, 10 and 5 μm particles selectively migrated to inner and outer outlets, demonstrating separation efficiencies of 99.7% and 90.3%, respectively. With duplex samples, sample purity was measured to be 93.4% and 98.6% for 10 and 5 μm particles collected from the inner and the outer outlets, respectively. Application of our device in biological assays was shown by performing duplex experiments where 10 μm particles were isolated from Salmonella bacterial suspension with purity of 97.8% while increasing the state-of-the-art particle solution exchange and separation throughput by 16 folds. This parallelization enabled desirable combinations of high throughput, low-cost, and scalability, without compromising efficiency and purity, paving the way for sample preparation at the PoN in the future.

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通过微流控离心机的平面内和平面外并行化,同时进行高通量颗粒-细菌分离和溶液交换。
惯性微流控装置在需求点(PoN)样品制备方面受到了广泛关注。然而,能够同时进行颗粒-细菌溶液交换和分离的设备吞吐量较低,阻碍了它们在 PoN 环境中的应用。本文介绍了一种用于高通量溶液交换和微颗粒分离的微流体离心机,以满足以较高流速处理大量样品的需求。该装置通过平面内和平面外并行化,在 24 个组装成三层结构的弯曲微通道内集成了微颗粒的迪安流再循环和惯性聚焦。我们在具有不同曲线数(2 曲线、8 曲线和 24 曲线)的装置中使用单重和双重样品研究了溶液交换和微粒迁移。以最高 16.8 毫升/分钟的流速处理 5 微米和 10 微米微粒,溶液交换效率达到 96.69%。在单倍溶液中,10 微米和 5 微米微粒选择性地迁移到内出口和外出口,分离效率分别为 99.7% 和 90.3%。对于双联样品,从内出口和外出口收集到的 10 微米和 5 微米颗粒的样品纯度分别为 93.4% 和 98.6%。我们的设备在生物检测中的应用体现在双工实验中,从沙门氏菌细菌悬浮液中分离出的 10 μm 颗粒纯度高达 97.8%,同时将最先进的颗粒溶液交换和分离吞吐量提高了 16 倍。这种并行化实现了高通量、低成本和可扩展性的理想组合,同时不影响效率和纯度,为未来在 PoN 进行样品制备铺平了道路。
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来源期刊
Biomicrofluidics
Biomicrofluidics 生物-纳米科技
CiteScore
5.80
自引率
3.10%
发文量
68
审稿时长
1.3 months
期刊介绍: Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...
期刊最新文献
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